AB8635

Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]

Name: Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) | Abcam
Brand: Abcam Limited
SKU: AB8635
Price: 545 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(8 Publications)

Mouse Monoclonal Hepatitis B Virus pre S2 Antigen antibody. Suitable for IP, ELISA, WB, IHC-FoFr, IHC-P, IM, IHC-Fr and reacts with Hepatitis B virus, Transfected cell lysate - Hepatitis B virus samples. Cited in 8 publications.

View Alternative Names

HBV pre S2 Antigen, HBV pre S2 protein, Hepatitis B Virus pre S2 protein, PreS2/S antigen

1 Images

AbReview23877****

Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (AB8635)

All lanes:

Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution

Lane 1:

Whole cell lysate prepared from HEK cells at 50 µg

Lanes 2 - 3:

Whole cell lysate prepared from HEK cells transfected with pCI M at 50 µg

Secondary

All lanes:

Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution

Observed band size: 33 kDa,36 kDa,39 kDa

true

Exposure time: 10min

Image courtesy of Alina Macovei by Abreview.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S26

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Hepatitis B virus

Applications

IM, IHC-Fr, ELISA, IHC-FoFr, WB, IP, IHC-P

applications

Epitope

aa positions 131-139 (adw nomenclature)

Specificity

This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IM" : {"fullname" : "Immunomicroscopy", "shortname":"IM"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "1/100", "IHCFoFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "IM-species-checked": "guaranteed", "IM-species-dilution-info": "", "IM-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" }, "Transfected cell lysate - Hepatitis B virus": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IM-species-checked": "notRecommended", "IM-species-dilution-info": "", "IM-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis B Virus pre S2 antigen also known simply as pre S2 is a component of the Hepatitis B surface antigen (HBsAg). It plays a role in the viral life cycle and assists in the infection process of liver cells. The pre S2 antigen has a molecular mass of approximately 31 kDa. It is expressed on the surface of infected liver cells and virions where it facilitates the entry of the virus into the host cells.

Biological function summary

The pre S2 antigen is an integral part of the Hepatitis B viral structure participating in the initial attachment and penetration into hepatocytes. The pre S2 region of HBsAg is known for containing receptor-binding domains that interact with cellular surface molecules aiding in viral adherence and entry. These interactions are important for establishing infection making the pre S2 region a critical determinant in viral infectivity and host specificity.

Pathways

The pre S2 antigen plays a role in viral entry pathways involving liver-specific processes. During the Hepatitis B virus life cycle the pre S2 antigen interacts with the sodium taurocholate co-transporting polypeptide (NTCP) a transporter on hepatocytes which is essential for the virus's entry and subsequent replication within liver cells. This interaction highlights the importance of the antigen in the viral infection pathway and its involvement in the viral-host dynamics.

The pre S2 antigen is directly linked to Hepatitis B infection a major cause of liver disease including cirrhosis and hepatocellular carcinoma. Chronic infection with Hepatitis B and the presence of pre S2 can lead to serious liver damage over time. During the progression of these diseases elevated expression of pre S2 antigen is observed further influencing disease severity. The antigen’s role in facilitating viral entry and replication makes it a target for therapeutic interventions aimed at alleviating Hepatitis B-related liver disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Hepatitis B Virus pre S2 Antigen

style

left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013391 PubMed40763308

2025

HBV capsid assembly modulators differentially modulate the assembly of wild-type and drug-resistant core protein chimeric nucleocapsids and empty capsids.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Hemraj Rimal,Jun Lyu,Liangxian Shen,Qiong Zhao,Jinhong Chang,Ju-Tao Guo

Nature communications 14:8440 PubMed38114531

2023

NDP52 mediates an antiviral response to hepatitis B virus infection through Rab9-dependent lysosomal degradation pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuzhi Cui,Tian Xia,Jianjin Zhao,Xiaoyu Ren,Tingtao Wu,Mireille Kameni,Xiaoju Guo,Li He,Jingao Guo,Aléria Duperray-Susini,Florence Levillayer,Jean-Marc Collard,Jin Zhong,Lifeng Pan,Frédéric Tangy,Pierre-Olivier Vidalain,Dongming Zhou,Yaming Jiu,Mathias Faure,Yu Wei

Journal of immunology (Baltimore, Md. : 1950) 208:672-684 PubMed35022275

2022

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Li,Xiaoqiong Duan,Chuanlong Zhu,Xiao Liu,Andre J Jeyarajan,Min Xu,Zeng Tu,Qiuju Sheng,Dong Chen,Chuanwu Zhu,Tuo Shao,Zhimeng Cheng,Shadi Salloum,Esperance A Schaefer,Annie J Kruger,Jacinta A Holmes,Raymond T Chung,Wenyu Lin

Human vaccines & immunotherapeutics 16:756-778 PubMed31687879

2019

A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Allan Ma,Bruce Motyka,Klaus Gutfreund,Yuenian Eric Shi,Rajan George

PloS one 11:e0159089 PubMed27434097

2016

Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-κB Pathways in an ER Stress-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yang-Xia Li,Yan-Li Ren,Hai-Jing Fu,Ling Zou,Ying Yang,Zhi Chen

ACS nano 6:7634-43 PubMed22934963

2012

Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Shehata Draz,Binbin Amanda Fang,Lanjuan Li,Zhi Chen,Yingjie Wang,Yuhong Xu,Jun Yang,Kevin Killeen,Fanqing Frank Chen

Gastroenterology 136:168-176.e2 PubMed18996386

2008

In vitro characterization of viral fitness of therapy-resistant hepatitis B variants.

Applications

ICC/IF

Species

Unspecified reactive species

Stéphanie Villet,Gaëtan Billioud,Christian Pichoud,Julie Lucifora,Olivier Hantz,Camille Sureau,Paul Dény,Fabien Zoulim

Journal of virology 77:9511-21 PubMed12915565

2003

Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus.

Applications

Unspecified application

Species

Unspecified reactive species

Dieter Glebe,Mehriar Aliakbari,Peter Krass,Eva V Knoop,Klaus P Valerius,Wolfram H Gerlich

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]

Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (AB8635)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Epitope

Specificity

Reactivity data

Properties and storage information

Form

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise