Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)

Rabbit Recombinant Monoclonal Protein X antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus, Transfected cell line - Hepatitis B virus, Recombinant fragment - Hepatitis B virus samples.

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Images

Western blot - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (AB309353), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	Flow Cyt (Intra)	ICC/IF	IHC-P	WB	I-ELISA
Hepatitis B virus	Predicted	Predicted	Predicted	Predicted	Predicted	Predicted
Recombinant fragment - Hepatitis B virus	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended	Tested
Transfected cell line - Hepatitis B virus	Not recommended	Tested	Tested	Tested	Not recommended	Not recommended
Transfected cell lysate - Hepatitis B virus	Tested	Not recommended	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Hepatitis B virus, Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Hepatitis B virus	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Hepatitis B virus	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell line - Hepatitis B virus	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Hepatitis B virus, Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Hepatitis B virus	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Hepatitis B virus	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Hepatitis B virus, Transfected cell line - Hepatitis B virus	Dilution info -	Notes -

Target data

See full target information X

Function

Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC). Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation. This host complex would otherwise bind to viral episomal DNA, and prevents its transcription. Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT.

Alternative names

Protein X, HBx, Peptide X, pX, X

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR27041-49
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Protein X also known as HBV protein X is a multifunctional protein with a molecular mass of approximately 17 kDa. It is an integral component of Hepatitis B virus (HBV) and expressed within infected host liver cells. Protein X influences viral replication by interacting with host cellular mechanisms specifically modulating transcriptional regulation and signaling pathways that can benefit viral persistence. Due to its versatile nature researchers study Protein X's diverse roles in the context of HBV infection.

Biological function summary

The function of Protein X extends beyond viral replication. It plays a significant role in manipulating the host's immune response often suppressing antiviral activities which can allow the virus to evade the immune system. The interactions with cellular proteins may not always be direct as Protein X can be part of a larger viral replication complex. These interactions impact the host cell’s regulatory networks complicating the response to infection and facilitating chronic hepatitis conditions.

Pathways

Protein X is involved in the regulation of cellular apoptosis and the modulation of the NF-kB signaling pathway. These pathways contribute to the survival and proliferation of infected cells and Protein X often interacts with host proteins like p53 and CREB to exert its effects. By affecting these pathways Protein X ensures the survival of the virus within the host promoting pathological conditions.

Associated diseases and disorders

Protein X's impact is most closely associated with chronic Hepatitis B and related liver diseases. The protein's involvement in hepatocarcinogenesis highlights its oncogenic potential since it can promote the development of hepatocellular carcinoma (HCC). Interactions with proteins such as p53 highlight its role in oncogenic processes disrupting cell cycle regulation and apoptosis which can lead to uncontrolled cell growth and cancer progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
Hepatitis B virus Protein X Western blot staining using rabbit Anti-Hepatitis B virus Protein X antibody
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

In Western blot, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.
All lanes: Western blot - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] (Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a myc-His-tag® whole cell lysate
Lane 2: 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containi a myc-His-tag® whole cell lysate
Lane 3: 293T transfected with Hepatitis B Virus X antigen (genotype B1) expression vector containi a myc-His-tag® whole cell lysate
Lane 4: 293T transfected with Hepatitis B Virus X antigen (genotype C) expression vector containi a myc-His-tag® whole cell lysate
Lane 5: 293T transfected with Hepatitis B Virus X antigen (genotype D) expression vector containi a myc-His-tag® whole cell lysate
Lane 6: 293T transfected with Hepatitis B Virus X antigen (genotype E) expression vector containi a myc-His-tag® whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 15 kDa, 17 kDa
Exposure time: 59s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human tissue labeling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/4000 (0.131 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with an HBxA2 expression vector containing a His tag, (B) HEK-293T transfected with an HBxB1 expression vector containing a His tag, (C) HEK-293T transfected with an HBxC expression vector containing a His tag, (D) HEK-293T transfected with an HBxD expression vector containing a His tag, and (E) HEK-293T transfected with an HBxE expression vector containing a His tag, no staining on (F) HEK-293T transfected with an empty vector containing a His tag. The section was incubated with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunoprecipitation - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.

Hepatitis B virus Protein X was immunoprecipitated from 0.35 mg 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc-His-tag® whole cell lysate with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

Lane 1: 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc-His-tag® whole cell lysate

Lane 2: Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 IP in 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc-His-tag® whole cell lysate

Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 in 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc-His-tag® whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] (Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352) at 1/30 dilution
All lanes: 293T transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc-His-tag® whole cell lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 17 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/4000 (0.131 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on rat liver. The section was incubated with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/4000 (0.131 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on mouse liver. The section was incubated with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/4000 (0.131 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/500 (1.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining both in 293T cells transfected with Hepatitis B Virus X antigen (genotype D) expression vector containing a myc tag and in 293T cells transfected with Hepatitis B Virus X antigen (genotype E) expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/500 (1.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining both in 293T cells transfected with Hepatitis B Virus X antigen (genotype B1) expression vector containing a myc tag and in 293T cells transfected with Hepatitis B Virus X antigen (genotype C) expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/500 (1.046 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing positive staining in 293T cells transfected with Hepatitis B Virus X antigen (genotype A2) expression vector containing a myc tag and no staining in 293T cells transfected with empty vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Myc-Tag Mouse mab (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
Flow Cytometry (Intracellular) - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with a empty vector / Hepatitis B virus Protein X (genotype A2)/ Hepatitis B virus Protein X (genotype B1) / Hepatitis B virus Protein X (genotype C) / Hepatitis B virus Protein X (genotype D) / Hepatitis B virus Protein X (genotype E) expression vector containing a His tag cells labelling Hepatitis B virus Protein X with Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352 at 1/500 dilution (0.1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Indirect ELISA - Anti-Hepatitis B virus Protein X antibody [EPR27041-49] - BSA and Azide free (ab309353)
This data was developed using Anti-Hepatitis B virus Protein X antibody [EPR27041-49] ab309352, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of abab309352 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Protein X.
Antigen concentration: 1000 ng/ml