AB9193

Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

Name: Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab9193) | Abcam
Brand: Abcam Limited
SKU: AB9193
Price: 300 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(41 Publications)

Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab9193) is a horse polyclonal antibody detecting Hepatitis B Virus Surface Antigen (Ad/Ay) in ELISA. Suitable for Hepatitis B virus.

- Over 30 publications
- Trusted since 2002

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S, Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S, Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

1 Images

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (AB9193)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (AB9193)

Indirect ELISA analysis of ab9193. Primary antibody incubated for 60 minutes. The Secondary antibody used was a HRP conjugated Goat anti-Horse IgG Fc incubated for 30 minutes. Antigen (Hepatitis B) incubated for 60 minutes (1/750 dilution). Plates were blocked using 1% BSA. Plates developed using TMB substrate. Plates were read using a microplate reader with absorbance set at 450 nm.

Key facts

Host species

Horse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Hepatitis B virus

Applications

I-ELISA

applications

Specificity

This antibody is specific for Hepatitis B surface antigen

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (ab9193) is a horse polyclonal antibody and is validated for use in ELISA in Hepatitis B virus samples.

Trusted by the scientific community
Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) (ab9193) was first used in a scientific publication in 2002 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This antibody was immunoaffinity purified from non-recombinant hepatitis antigens.

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen (HBsAg) also known as Hepatitis B surface antigen AD/AY serves as a significant marker for the Hepatitis B virus (HBV). This glycoprotein displays a molecular mass of approximately 24 kDa and consists of small medium and large protein forms. HBsAg is typically expressed in the infected liver cells and secreted into the bloodstream. This expression allows its use as a diagnostic marker for HBV infection through assays like HBsAg ELISA which detects the presence of the virus.

Biological function summary

The surface antigen plays an important role in the virus's attachment and entry into hepatocytes. HBsAg forms part of the viral envelope structure which facilitates the virus's ability to infect host cells. The presence of HBsAg is linked to an individual’s immune response specifically the production of hepatitis antibodies. Its interaction with the host’s immune system and antibodies like hep B antibodies are essential for understanding both viral pathogenesis and immunity to HBV infections.

Pathways

The Hepatitis B virus surface antigen plays critical parts in the infectious cycle and immune response pathways. One important pathway includes the endocytosis pathway where HBsAg is involved in the virus's entry into liver cells. The surface antigen also interacts with the human immune system influencing the expression of Hepatitis B antibodies. Connected proteins include those involved in the immune signaling where alterations affect the body's ability to mount an effective response against HBV.

The Hepatitis B Virus Surface Antigen is directly associated with Hepatitis B infection and its progression. Chronic HBV infection may lead to liver-related diseases such as cirrhosis and hepatocellular carcinoma. HBsAg presence serves as an indicator for diagnosing these liver diseases. The antigen's interaction with other viral proteins such as the core antigen and E antigen are important in the disease's progression and overall clinical outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

See full target information S

Additional targets

S,S

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1013391 PubMed40763308

2025

HBV capsid assembly modulators differentially modulate the assembly of wild-type and drug-resistant core protein chimeric nucleocapsids and empty capsids.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Hemraj Rimal,Jun Lyu,Liangxian Shen,Qiong Zhao,Jinhong Chang,Ju-Tao Guo

Hepatology communications 9: PubMed40079732

2025

Amphisome plays a role in HBV production and release through the endosomal and autophagic pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Li,Thekla Kemper,Ruth Broering,Yong Lin,Xueyu Wang,Mengji Lu

ACS omega 10:484-497 PubMed39829464

2025

Inhibition of Hepatitis B Virus Replication by a Novel GalNAc-siRNA In Vivo and In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Zhang,Yanqin Ma,Yan He,Dong Wang,Kun Yue,Xiaomei Zhang,Huaien Song

Acta biochimica et biophysica Sinica 57:486-496 PubMed39722572

2024

The host gene regulates HBV replication via HBV PRE-induced nuclear export.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Wang,Jing Li,Wentao Xie,Zhongliang Shen,Jingwen Wu,Richeng Mao,Mengji Lu,Jiming Zhang

Journal of medical virology 96:e29614 PubMed38647071

2024

Multi-omics panoramic analysis of HBV integration, transcriptional regulation, and epigenetic modifications in PLC/PRF/5 cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Guiwen Guan,Abudurexiti Abulaiti,Chenxiao Qu,Chia-Chen Chen,Zhiqiang Gu,Jing Yang,Ting Zhang,Xiaojie Chen,Zhao Zhou,Fengmin Lu,Xiangmei Chen

Journal of virology 98:e0150223 PubMed38315015

2024

Class A capsid assembly modulator apoptotic elimination of hepatocytes with high HBV core antigen level is dependent on core protein translation.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Martin Berke,Ying Tan,Sarah Sauviller,Dai-Tze Wu,Ke Zhang,Nádia Conceição-Neto,Alfonso Blázquez Moreno,Desheng Kong,George Kukolj,Chris Li,Ren Zhu,Isabel Nájera,Frederik Pauwels

PloS one 18:e0293167 PubMed37910550

2023

Pangenomic antiviral effect of REP 2139 in CRISPR/Cas9 engineered cell lines expressing hepatitis B virus surface antigen.

Applications

Unspecified application

Species

Unspecified reactive species

Léna Angelo,Andrew Vaillant,Matthieu Blanchet,Patrick Labonté

Journal of virology 97:e0128723 PubMed37800948

2023

Oxidative stress sensor Keap1 recognizes HBx protein to activate the Nrf2/ARE signaling pathway, thereby inhibiting hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Adi Ariffianto,Lin Deng,Takayuki Abe,Chieko Matsui,Masahiko Ito,Akihide Ryo,Hussein Hassan Aly,Koichi Watashi,Tetsuro Suzuki,Masashi Mizokami,Yoshiharu Matsuura,Ikuo Shoji

Virology journal 20:159 PubMed37468949

2023

Host sex disparity and viral genotype dependence of the glycosylation level of small Hepatitis B surface protein in patients with HBeAg-positive chronic Hepatitis B.

Applications

Unspecified application

Species

Unspecified reactive species

Guomin Ou,Chengyu Zhao,Juan Deng,Hui Zhuang,Kuanhui Xiang,Tong Li

Microbiology and immunology 67:281-292 PubMed36929353

2023

Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chie Aoki-Utsubo,Puguh Indrasetiawan,Kento Fukano,Masamichi Muramatsu,Nina Artanti,Muhammad Hanafi,Hak Hotta,Masanori Kameoka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (AB9193)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Alternative Products

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Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (41)

Product promise