AB859

Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted

Name: Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859) | Abcam
Brand: Abcam Limited
SKU: AB859
Price: 430 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal HBSAG antibody. Suitable for IHC-P and reacts with Hepatitis B virus samples. Cited in 4 publications.

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (AB859)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (AB859)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hepatitis B infected liver tissue labeling Hepatitis B Virus Surface Antigen with ab859.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E7

Isotype

IgG1

Carrier free

Reacts with

Hepatitis B virus

Applications

IHC-P

applications

Specificity

This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/50", "IHCP-species-notes": "<p>Pretreat tissue with citrate buffer pH 6. Incubate with primary antibody for 30 minutes at room temperature.</p>" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen commonly known as HBsAg is an important structural protein of the Hepatitis B Virus (HBV). This antigen is also referred to as Hep B surf Ab. Designed with a mass of approximately 24 kDa HBsAg is expressed extensively in the liver tissues of individuals infected with Hepatitis B. It represents the major component of the virus's envelope and plays a role in the formation of subviral particles. Several commercial antibodies such as Hepatitis antibodies and HBV antibodies target HBsAg for analytical and diagnostic purposes in laboratories.

Biological function summary

The Hepatitis B Virus Surface Antigen interacts with the host's immune system and assists in viral replication. HBsAg is not part of a larger protein complex. Rather it circulates as free particles known as 3e-7 and 3e-7 Hepatitis B antigens which are abundant in the blood serum during active infection. These particles act as decoys distracting the host's immune defense and facilitating the persistence of the infection. The production and clearance of HBsAg serve as important indicators of HBV infection status and disease progression.

Pathways

Hepatitis B Virus Surface Antigen plays an integral role in the HBV life cycle particularly within the viral entry and replication pathways. It shares a functional relationship with the HB5 protein which is important for viral particle assembly and release. The interactions between HBsAg and host proteins influence viral propagation and subsequently the overall pathway of HBV infection within hepatocytes. Understanding these interactions can aid in the development of therapeutic interventions to disrupt the viral lifecycle.

Hepatitis B Virus Surface Antigen is directly associated with chronic Hepatitis B infection and hepatocellular carcinoma. Chronic Hepatitis B characterized by the prolonged presence of HBsAg can advance to liver cirrhosis and cancer if untreated. The antigen's persistence is often monitored alongside liver enzymes and other markers such as Hepatitis B antibodies (Hep B antibodies) to assess disease progression and treatment efficacy. Effective management of HBsAg levels helps mitigate the risk and severity of these severe liver diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

See full target information S

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 98:e0150223 PubMed38315015

2024

Class A capsid assembly modulator apoptotic elimination of hepatocytes with high HBV core antigen level is dependent on core protein translation.

Applications

Unspecified application

Species

Unspecified reactive species

Jan Martin Berke,Ying Tan,Sarah Sauviller,Dai-Tze Wu,Ke Zhang,Nádia Conceição-Neto,Alfonso Blázquez Moreno,Desheng Kong,George Kukolj,Chris Li,Ren Zhu,Isabel Nájera,Frederik Pauwels

Vaccines 11: PubMed38140229

2023

Sustained Liver HBsAg Loss and Clonal T- and B-Cell Expansion upon Therapeutic DNA Vaccination Require Low HBsAg Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Nádia Conceição-Neto,Wim Pierson,Maurizio Vacca,Matthias Beyens,Ben De Clerck,Liese Aerts,Birgit Voeten,Dorien De Pooter,Lore Verschueren,Koen Dockx,Mathias Vandenberk,Ewoud De Troyer,Kato Verwilt,Carl Van Hove,Mieke Verslegers,Leslie Bosseler,Marjolein Crabbe,Vinod Krishna,Isabel Nájera,Ellen Van Gulck

European journal of gastroenterology & hepatology 33:76-82 PubMed32049678

2020

Expression of hepatitis B surface antigen in liver tissues can serve as a predictor of prognosis for hepatitis B virus-related hepatocellular carcinoma patients after liver resection.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming He,Jinbin Chen,Juncheng Wang,Li Xu,Zhongguo Zhou,Minshan Chen,Yaojun Zhang,Mude Shi

Stem cell research 9:35-48 PubMed22640926

2012

DLK1(PREF1) is a negative regulator of adipogenesis in CD105⁺/CD90⁺/CD34⁺/CD31⁻/FABP4⁻ adipose-derived stromal cells from subcutaneous abdominal fat pats of adult women.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Mitterberger,Stefan Lechner,Monika Mattesich,Andreas Kaiser,Daniela Probst,Nikolaus Wenger,Gerhard Pierer,Werner Zwerschke

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (AB859)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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