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Rabbit Recombinant Monoclonal HBSAG antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979, Transfected cell line - Hepatitis B virus adw2/Rutter/1979, Recombinant fragment - Hepatitis B virus adw2/Rutter/1979 samples.

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Images

Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (AB322461), expandable thumbnail
  • Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (AB322461), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (AB322461), expandable thumbnail
  • Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (AB322461), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (AB322461), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFI-ELISA
Hepatitis B virus adw2/Rutter/1979
Predicted
Predicted
Predicted
Predicted
Recombinant fragment - Hepatitis B virus adw2/Rutter/1979
Not recommended
Not recommended
Not recommended
Tested
Transfected cell line - Hepatitis B virus adw2/Rutter/1979
Not recommended
Tested
Tested
Not recommended
Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Hepatitis B virus adw2/Rutter/1979, Recombinant fragment - Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Hepatitis B virus adw2/Rutter/1979
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979, Recombinant fragment - Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Hepatitis B virus adw2/Rutter/1979
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979, Recombinant fragment - Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Hepatitis B virus adw2/Rutter/1979
Dilution info
62.5 ng/mL
Notes

-

Predicted
Predicted

Species
Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979, Transfected cell line - Hepatitis B virus adw2/Rutter/1979
Dilution info
-
Notes

-

Target data

Function

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

Alternative names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

Rabbit Recombinant Monoclonal HBSAG antibody. Suitable for WB, IHC-P, ICC/IF, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus adw2/Rutter/1979, Transfected cell line - Hepatitis B virus adw2/Rutter/1979, Recombinant fragment - Hepatitis B virus adw2/Rutter/1979 samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29887-532
Purification technique
Affinity purification Protein A
Specificity

This antibody reacts with Hepatitis B virus Large (L) envelope protein from genotype A2 but not genotype H, D or A1.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461), expandable thumbnail

    Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody reacts with Hepatitis B virus Large (L) envelope protein from genotype A2 but not genotype H, D or A1.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Hepatitis B virus genotype A2 Large (L) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a Hepatitis B virus genotype H Large (L) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a Hepatitis B virus genotype D Large (L) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a Hepatitis B virus genotype A1 Large (L) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 44 kDa, 36 kDa

    Exposure time: 48s

  • Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461), expandable thumbnail

    Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with overexpressed Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle (M) envelope protein and Small (S) envelope protein.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle (M) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Small (S) envelope protein expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 44 kDa, 36 kDa

    Exposure time: 180s

  • Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling HBV envelope L protein with ab322461 at 1/50 (10.12 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/ 1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/ 100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.

  • Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461), expandable thumbnail

    Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461)

    Indirect ELISA analysis of ab322461 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.

    Antigen: Large envelope protein.

    Antigen concentration: 1000 ng/ml

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis B Virus Surface Antigen antibody [EPR29887-532] (ab322461)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. (B) HEK-293T transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle (M) envelope protein expression vector containing a myc-His-tag®. (C) HEK-293T transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Small (S) envelope protein expression vector containing a myc-His-tag®. (D) HEK-293T transfected with an empty vector containing a His-tag®. tissue labeling HBV envelope L protein with ab322461 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in (A) HEK-293T transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Large (L) envelope protein expression vector containing a myc-His-tag®. No staining in (B) HEK-293T transfected with Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Middle (M) envelope protein expression vector containing a myc-His-tag®, (C) HEK-293T transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) Small (S) envelope protein expression vector containing a myc-His-tag® and (D) HEK-293T transfected with an empty vector containing a His-tag.

    The section was incubated with ab322461 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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