AB8333

Anti-Hepatitis B Virus Surface Antigen antibody [HB5]

Name: Anti-Hepatitis B Virus Surface Antigen antibody [HB5] (ab8333) | Abcam
Brand: Abcam Limited
SKU: AB8333
Price: 525 USD
Availability: InStock

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Mouse Monoclonal HBSAG antibody. Suitable for ICC, ELISA and reacts with Hepatitis B virus samples.

View Alternative Names

Large envelope protein, L glycoprotein, L-HBsAg, Large S protein, Large surface protein, Major surface antigen, LHB, S

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

HB5

Isotype

IgG2a

Carrier free

Reacts with

Hepatitis B virus

Applications

ELISA, ICC

applications

Specificity

Recognizes following Hepatitis B virus subtypes:ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq- Specificity of MAb HB5 was verified by ELISA on panel of International Workshop on HBsAg Subtypes (Paris, April 1975). This antibody does not cross-block with ab8256 and is therefore suitable for use with ab8256 as a matched pair.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

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Why is this recommended?

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis B Virus Surface Antigen commonly known as HBsAg is an important structural protein of the Hepatitis B Virus (HBV). This antigen is also referred to as Hep B surf Ab. Designed with a mass of approximately 24 kDa HBsAg is expressed extensively in the liver tissues of individuals infected with Hepatitis B. It represents the major component of the virus's envelope and plays a role in the formation of subviral particles. Several commercial antibodies such as Hepatitis antibodies and HBV antibodies target HBsAg for analytical and diagnostic purposes in laboratories.

Biological function summary

The Hepatitis B Virus Surface Antigen interacts with the host's immune system and assists in viral replication. HBsAg is not part of a larger protein complex. Rather it circulates as free particles known as 3e-7 and 3e-7 Hepatitis B antigens which are abundant in the blood serum during active infection. These particles act as decoys distracting the host's immune defense and facilitating the persistence of the infection. The production and clearance of HBsAg serve as important indicators of HBV infection status and disease progression.

Pathways

Hepatitis B Virus Surface Antigen plays an integral role in the HBV life cycle particularly within the viral entry and replication pathways. It shares a functional relationship with the HB5 protein which is important for viral particle assembly and release. The interactions between HBsAg and host proteins influence viral propagation and subsequently the overall pathway of HBV infection within hepatocytes. Understanding these interactions can aid in the development of therapeutic interventions to disrupt the viral lifecycle.

Hepatitis B Virus Surface Antigen is directly associated with chronic Hepatitis B infection and hepatocellular carcinoma. Chronic Hepatitis B characterized by the prolonged presence of HBsAg can advance to liver cirrhosis and cancer if untreated. The antigen's persistence is often monitored alongside liver enzymes and other markers such as Hepatitis B antibodies (Hep B antibodies) to assess disease progression and treatment efficacy. Effective management of HBsAg levels helps mitigate the risk and severity of these severe liver diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.. The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

See full target information S

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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