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Mouse Recombinant Monoclonal HBSAG antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus samples.

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Images

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (AB319998), expandable thumbnail
  • Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (AB319998), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBI-ELISA
Hepatitis B virus
Predicted
Predicted
Recombinant fragment - Hepatitis B virus
Not recommended
Tested
Transfected cell lysate - Hepatitis B virus
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Hepatitis B virus
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Hepatitis B virus
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Hepatitis B virus
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Hepatitis B virus
Dilution info
62.5 ng/mL
Notes

-

Predicted
Predicted

Species
Hepatitis B virus
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Hepatitis B virus
Dilution info
-
Notes

-

Target data

Alternative names

HBsAg

Mouse Recombinant Monoclonal HBSAG antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Hepatitis B virus, Recombinant fragment - Hepatitis B virus samples.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
2D11
Purification technique
Affinity purification Protein A
Specificity

This antibody recognizes both Hepatitis B Virus Surface Antigen from strains ayw and ayr, but do not cross-react with stains adw2 and adr.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (ab319998), expandable thumbnail

    Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (ab319998)

    Indirect ELISA analysis of ab319998 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution.

    Antigen: HBsAg ayw Subtype,HBsAg adw Subtype.

    Antigen concentration: 5000 ng/ml

  • Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (ab319998), expandable thumbnail

    Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (ab319998)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw and ayr) antibody [2D11] (ab319998) at 1/1000 dilution

    Lane 1: 293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a Hepatitis B virus genotype C subtype ayr (isolate Human/Japan/Okamoto) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a Hepatitis B virus genotype C subtype adr (isolate Japan/Nishioka/1983) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 40 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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