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AB322870

Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10]

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Mouse Recombinant Monoclonal HBSAG antibody. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Hepatitis B virus, Transfected cell lysate - Hepatitis B virus samples.

View Alternative Names

LHB, Large envelope protein, Large surface protein, Major surface antigen

2 Images
Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)

Indirect ELISA analysis of ab322870 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979).

Antigen concentration : 1000-0 ug/ml

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)
  • WB

Supplier Data

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (AB322870)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (ab322870) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Hepatitis B virus genotype C subtype ayr (isolate Human/Japan/Okamoto/-) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Hepatitis B virus genotype C subtype adr (isolate Japan/Nishioka/1983) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C10

Isotype

IgG1

Carrier free

No

Reacts with

Hepatitis B virus

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with overexpressed Hepatitis B Virus Surface Antigen from subtypes adw2, ayr and adr.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Hepatitis B virus": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2 µg/mL", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Hepatitis B virus": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Hepatitis B Virus Surface Antigen
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com