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AB324813

Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free

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Mouse Recombinant Monoclonal HBSAG antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Hepatitis B virus, Transfected cell lysate - Hepatitis B virus samples.

View Alternative Names

LHB, Large envelope protein, Large surface protein, Major surface antigen

2 Images
Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free (AB324813)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free (AB324813)

This data was developed using ab322870, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322870 at 2000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979).

Antigen concentration : 1000-0 ug/ml

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free (AB324813)
  • WB

Supplier Data

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] - BSA and Azide free (AB324813)

This data was developed using ab322870, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10] (<a href='/en-us/products/primary-antibodies/hepatitis-b-virus-surface-antigen-ayw-antibody-1c10-ab322870'>ab322870</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Hepatitis B virus genotype D subtype ayw (isolate France/Tiollais/1979) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Hepatitis B virus genotype A2 subtype adw2 (strain Rutter 1979) (HBV-A) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a Hepatitis B virus genotype C subtype ayr (isolate Human/Japan/Okamoto/-) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a Hepatitis B virus genotype C subtype adr (isolate Japan/Nishioka/1983) (HBV-C) expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

  • Unconjugated

    Anti-Hepatitis B Virus Surface Antigen (ayw) antibody [1C10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1C10

Isotype

IgG1

Carrier free

Yes

Reacts with

Hepatitis B virus

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody does not cross-react with overexpressed Hepatitis B Virus Surface Antigen from subtypes adw2, ayr and adr.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Hepatitis B virus": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Hepatitis B virus": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab324813 is the carrier-free version of ab322870.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Hepatitis B Virus Surface Antigen
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com