AB235

Anti-Hepatitis B Virus X antigen antibody [3F6-G10]

Name: Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235) | Abcam
Brand: Abcam Limited
SKU: AB235
Price: 505 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(14 Publications)

Mouse Monoclonal Protein X antibody. Suitable for ELISA, IHC-P, IHC-Fr and reacts with Hepatitis B virus samples. Cited in 14 publications.

View Alternative Names

HBV X antigen, HBx, HBxAg

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F6-G10

Isotype

IgG2a

Light chain type

unknown

Carrier free

Reacts with

Hepatitis B virus

Applications

IHC-P, IHC-Fr, ELISA

applications

Specificity

HB-X antigen (17 kD). We have received mixed reports regarding how ab235 works in Western blot, and we are not able to guarantee this antibody in WB.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis B Virus X antigen also known as HBx protein or hepatitis X has a molecular mass of approximately 17 kDa. This antigen is expressed in liver cells or hepatocytes infected with the hepatitis B virus (HBV). HBx protein plays an important role in the replication and pathogenesis of HBV influencing the virus lifecycle and its ability to cause liver disease. Additionally anti-HBx antibodies such as 'anti HBx' and '3f6' are significant for research and diagnostic purposes due to their binding specificity.

Biological function summary

The function of HBx protein is to modulate various cellular processes to benefit the virus. HBx is not part of a larger protein complex but it interacts with host cell pathways to promote viral replication and survival. It influences transcriptional activation cell cycle progression and apoptosis. By hijacking the host cellular machinery HBx protein enhances viral replication and contributes to chronic infection.

Pathways

The activity of HBx protein impacts critical cellular signaling networks. HBx can activate the NF-kB signaling pathway which plays a role in immune response and inflammation. Additionally HBx influences the Wnt/β-catenin signaling pathway affecting cell proliferation and differentiation. Through these pathways HBx interacts with multiple other proteins including c-Myc and p53 impacting cellular homeostasis and contributing to oncogenic potential.

HBx protein is associated with hepatitis B-related liver diseases and the development of hepatocellular carcinoma (HCC). HBx contributes significantly to the progression from chronic hepatitis B infection to liver cirrhosis and HCC. The protein affects tumor suppressors like p53 further enhancing its role in carcinogenesis. Understanding HBx’s mechanisms continues to be important for developing therapeutics and diagnostics aimed at treating HBV-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Hepatitis B Virus X antigen

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 12:98 PubMed35765066

2022

DNA methyltransferase 1 knockdown reverses PTEN and VDR by mediating demethylation of promoter and protects against renal injuries in hepatitis B virus-associated glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Haochen Guan,Nan Zhu,Gang Tang,Yi Du,Ling Wang,Weijie Yuan

Frontiers in oncology 11:625169 PubMed33767993

2021

NEDD4 Induces K48-Linked Degradative Ubiquitination of Hepatitis B Virus X Protein and Inhibits HBV-Associated HCC Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wan,Zhao Lei,Biao Tu,Tianyin Wang,Jiale Wang,Feizhou Huang

Inflammation research : official journal of the Eu 69:683-696 PubMed32347316

2020

Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hui Xie,Jian Ding,Xiao-Xia Xie,Xiao-Huang Yang,Xiao-Fan Wu,Zhi-Xin Chen,Qi-Lan Guo,Wen-Yu Gao,Xiao-Zhong Wang,Dan Li

Journal of experimental orthopaedics 7:10 PubMed32146609

2020

Enhancement of chondrogenic differentiation supplemented by a novel small compound for chondrocyte-based tissue engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Shuichi Hamamoto,Ryota Chijimatsu,Kazunori Shimomura,Masato Kobayashi,George Jacob,Fumiko Yano,Taku Saito,Ung-Il Chung,Sakae Tanaka,Norimasa Nakamura

JAMA network open 2:e195718 PubMed31199446

2019

Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types.

Applications

Unspecified application

Species

Unspecified reactive species

Ci Song,Jun Lv,Yao Liu,Jian Guo Chen,Zijun Ge,Jian Zhu,Juncheng Dai,Ling-Bin Du,Canqing Yu,Yu Guo,Zheng Bian,Ling Yang,Yiping Chen,Zhengming Chen,Jibin Liu,Jie Jiang,Liguo Zhu,Xiangjun Zhai,Yue Jiang,Hongxia Ma,Guangfu Jin,Hongbing Shen,Liming Li,Zhibin Hu

Molecular medicine reports 15:3285-3291 PubMed28339072

2017

Hepatitis B virus X protein increases microRNA‑21 expression and accelerates the development of hepatoma via the phosphatase and tensin homolog/phosphoinositide 3‑kinase/protein kinase B signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouhua Hou,Jun Quan

International journal of molecular medicine 38:513-20 PubMed27314843

2016

Hepatitis B virus X protein promotes renal epithelial-mesenchymal transition in human renal proximal tubule epithelial cells through the activation of NF-κB.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Li,Liping Hu,Fengxin Zhu,Zhangmei Zhou,Jianwei Tian,Jun Ai

Journal of experimental & clinical cancer research 35:87 PubMed27255760

2016

Hepatitis B virus whole-X and X protein play distinct roles in HBV-related hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zhang,Hongli Liu,Ruitian Yi,Taotao Yan,Yingli He,Yingren Zhao,Jinfeng Liu

PloS one 7:e41931 PubMed22848663

2012

Hepatitis B virus X protein upregulates mTOR signaling through IKKβ to increase cell proliferation and VEGF production in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Jui Yen,Yih-Jyh Lin,Chia-Sheng Yen,Hung-Wen Tsai,Ting-Fen Tsai,Kwang-Yu Chang,Wei-Chien Huang,Pin-Wen Lin,Chi-Wu Chiang,Ting-Tsung Chang

Oncology reports 28:127-32 PubMed22552576

2012

Apoptosis induced by hepatitis B virus X protein in a CCL13-HBx stable cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Chan-Yen Kuo,Ju-I Tsai,Tzu-Yu Chou,Man-Jung Hung,Cheng-Chung Wu,Shih-Lan Hsu,Guang-Yuh Hwang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus X antigen antibody [3F6-G10]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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