AB39716

Anti-Hepatitis B Virus X antigen antibody

Name: Anti-Hepatitis B Virus X antigen antibody (ab39716) | Abcam
Brand: Abcam Limited
SKU: AB39716
Price: 470 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(40 Publications)

Anti-Hepatitis B Virus X antigen antibody (ab39716) is a rabbit polyclonal antibody detecting Hepatitis B Virus X antigen in Western Blot, IP, ELISA. Suitable for Hepatitis B virus.

- Over 30 publications
- Trusted since 2006

View Alternative Names

HBV X antigen, HBx, HBxAg

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Hepatitis B virus

Applications

IP, ELISA, WB

applications

Specificity

The amino acid sequence of the recombinant human Hepatitis B Protein X is 100% homologous to the amino acid sequence of the natural Hepatitis B Protein X.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Hepatitis B Virus X antigen antibody (ab39716) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), ELISA in Hepatitis B virus samples.

What is the molecular weight of Hepatitis B Virus X antigen?
Anti-Hepatitis B Virus X antigen (ab39716) specifically detects a band for Hepatitis B Virus X antigen (UniProt: P12936) at a molecular weight of 17kDa.

Trusted by the scientific community
Anti-Hepatitis B Virus X antigen (ab39716) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Constituents: PBS, 0.58% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis B Virus X antigen also known as HBx protein or hepatitis X has a molecular mass of approximately 17 kDa. This antigen is expressed in liver cells or hepatocytes infected with the hepatitis B virus (HBV). HBx protein plays an important role in the replication and pathogenesis of HBV influencing the virus lifecycle and its ability to cause liver disease. Additionally anti-HBx antibodies such as 'anti HBx' and '3f6' are significant for research and diagnostic purposes due to their binding specificity.

Biological function summary

The function of HBx protein is to modulate various cellular processes to benefit the virus. HBx is not part of a larger protein complex but it interacts with host cell pathways to promote viral replication and survival. It influences transcriptional activation cell cycle progression and apoptosis. By hijacking the host cellular machinery HBx protein enhances viral replication and contributes to chronic infection.

Pathways

The activity of HBx protein impacts critical cellular signaling networks. HBx can activate the NF-kB signaling pathway which plays a role in immune response and inflammation. Additionally HBx influences the Wnt/Β-catenin signaling pathway affecting cell proliferation and differentiation. Through these pathways HBx interacts with multiple other proteins including c-Myc and p53 impacting cellular homeostasis and contributing to oncogenic potential.

HBx protein is associated with hepatitis B-related liver diseases and the development of hepatocellular carcinoma (HCC). HBx contributes significantly to the progression from chronic hepatitis B infection to liver cirrhosis and HCC. The protein affects tumor suppressors like p53 further enhancing its role in carcinogenesis. Understanding HBx’s mechanisms continues to be important for developing therapeutics and diagnostics aimed at treating HBV-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Hepatitis B Virus X antigen

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 22:189 PubMed40500754

2025

Lamin A/C promotes HBV transcription by modulating histone modification associated with cccDNA minichromosome in an HBx-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Linshan Jiang,Lanlang Peng,Hong Chen,Fangli Liao,Liping Yang,Yuanyuan Ye,Gaoli Zhang,Hua Sun,Qin Hu,Weixian Chen

Cancer medicine 14:e70916 PubMed40476478

2025

Hepatitis B Virus X Protein Upregulates SREBP2 to Modulate Autophagy in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyan Lin,Yongxu Lin,Yongzhu Huang,Mingrong Wang,Xiaoxia Xie,Weiqi Cai,Qilan Guo,Pingying Jiang,Yuanlin Qi,Dan Li

Journal of biomedical science 31:34 PubMed38561844

2024

Significance of hepatitis B virus capsid dephosphorylation via polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Chih-Hsu Chang,Chiaho Shih

Journal of virology 97:e0128723 PubMed37800948

2023

Oxidative stress sensor Keap1 recognizes HBx protein to activate the Nrf2/ARE signaling pathway, thereby inhibiting hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Adi Ariffianto,Lin Deng,Takayuki Abe,Chieko Matsui,Masahiko Ito,Akihide Ryo,Hussein Hassan Aly,Koichi Watashi,Tetsuro Suzuki,Masashi Mizokami,Yoshiharu Matsuura,Ikuo Shoji

BMC cancer 23:593 PubMed37370037

2023

Integrated analysis of intratumoral biomarker and tumor-associated macrophage to improve the prognosis prediction in cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Da Wang,Hao Xiang,Tian-Yu Hong,Abudurexiti Mierxiati,Fei-Hu Yan,Ling Zhang,Chao Wang

Frontiers in pharmacology 14:1159094 PubMed37361218

2023

Exploring the mechanism of JiGuCao capsule formula on treating hepatitis B virus infection via network pharmacology analysis and experiment verification.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Cao,Ningyi Zhang,Hening Chen,Wei Wang,Yijun Liang,Jiaxin Zhang,Ruijia Liu,Shuo Li,Yuhao Yao,Qian Jin,Ziwei Guo,Yue Chen,Yuanyuan Gong,Xiaoke Li,Xiaobin Zao,Yong'an Ye

World journal of gastroenterology 28:4812-4822 PubMed36156926

2022

Previous hepatitis B viral infection-an underestimated cause of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey Batskikh,Sergey Morozov,Alexey Dorofeev,Zanna Borunova,Dmitry Kostyushev,Sergey Brezgin,Anastasiya Kostyusheva,Vladimir Chulanov

Biochemistry and biophysics reports 32:101352 PubMed36160029

2022

HBx and YAP expression could promote tumor development and progression in HBV-related hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chiyumi Oda,Kenya Kamimura,Osamu Shibata,Shinichi Morita,Yuto Tanaka,Toru Setsu,Hiroyuki Abe,Takeshi Yokoo,Akira Sakamaki,Hiroteru Kamimura,Satoshi Kofuji,Toshifumi Wakai,Hiroshi Nishina,Shuji Terai

Viruses 14: PubMed36146694

2022

HBx 128-133 Deletion Affecting HBV Mother-to-Child Transmission Weakens HBV Replication via Reducing HBx Level and CP/ENII Transcriptional Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yarong Song,Ying Lu,Yi Li,Minmin Liu,Hui Zhuang,Jie Li,Jie Wang

Cellular and molecular gastroenterology and hepatology 14:1177-1198 PubMed35987451

2022

cccDNA Surrogate MC-HBV-Based Screen Identifies Cohesin Complex as a Novel HBV Restriction Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuanchang Wu,Liyuan Wang,Xin Wang,Yang Sun,Haoran Li,Zhaoying Zhang,Caiyue Ren,Xiaohui Zhang,Shuangjie Li,Jinghui Lu,Leiqi Xu,Xuetian Yue,Yue Hong,Qiang Li,Haizhen Zhu,Yaoqin Gong,Chengjiang Gao,Huili Hu,Lifen Gao,Xiaohong Liang,Chunhong Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus X antigen antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (40)

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