AB2741

Anti-Hepatitis B Virus X antigen antibody [X36C]

Name: Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741) | Abcam
Brand: Abcam Limited
SKU: AB2741

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(23 Publications)

Mouse Monoclonal Protein X antibody. Suitable for IP, ELISA, WB, IHC-P and reacts with Hepatitis B virus samples. Cited in 23 publications.

View Alternative Names

HBV X antigen, HBx, HBxAg

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

X36C

Isotype

IgG1

Carrier free

Reacts with

Hepatitis B virus

Applications

IHC-P, WB, ELISA, IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry paraffin embedded sections", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis B virus": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

undefined HPTLC

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis B Virus X antigen also known as HBx protein or hepatitis X has a molecular mass of approximately 17 kDa. This antigen is expressed in liver cells or hepatocytes infected with the hepatitis B virus (HBV). HBx protein plays an important role in the replication and pathogenesis of HBV influencing the virus lifecycle and its ability to cause liver disease. Additionally anti-HBx antibodies such as 'anti HBx' and '3f6' are significant for research and diagnostic purposes due to their binding specificity.

Biological function summary

The function of HBx protein is to modulate various cellular processes to benefit the virus. HBx is not part of a larger protein complex but it interacts with host cell pathways to promote viral replication and survival. It influences transcriptional activation cell cycle progression and apoptosis. By hijacking the host cellular machinery HBx protein enhances viral replication and contributes to chronic infection.

Pathways

The activity of HBx protein impacts critical cellular signaling networks. HBx can activate the NF-kB signaling pathway which plays a role in immune response and inflammation. Additionally HBx influences the Wnt/β-catenin signaling pathway affecting cell proliferation and differentiation. Through these pathways HBx interacts with multiple other proteins including c-Myc and p53 impacting cellular homeostasis and contributing to oncogenic potential.

HBx protein is associated with hepatitis B-related liver diseases and the development of hepatocellular carcinoma (HCC). HBx contributes significantly to the progression from chronic hepatitis B infection to liver cirrhosis and HCC. The protein affects tumor suppressors like p53 further enhancing its role in carcinogenesis. Understanding HBx’s mechanisms continues to be important for developing therapeutics and diagnostics aimed at treating HBV-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Hepatitis B Virus X antigen

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Cancer research 85:660-674 PubMed39652575

2024

HBV Remodels PP2A Complexes to Rewire Kinase Signaling in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Rigney E Turnham,Adriana Pitea,Gwendolyn M Jang,Zhong Xu,Huat Chye Lim,Alex L Choi,John Von Dollen,Rebecca S Levin,James T Webber,Elizabeth McCarthy,Junjie Hu,Xiaolei Li,Li Che,Ananya Singh,Alex Yoon,Gary K L Chan,Robin K Kelley,Danielle L Swaney,Wei Zhang,Sourav Bandyopadhyay,Fabian J Theis,Manon Eckhardt,Xin Chen,Kevan M Shokat,Trey Ideker,Nevan J Krogan,John D Gordan

Frontiers in pharmacology 15:1327149 PubMed38444939

2024

Exosomal miR-223-3p from bone marrow mesenchymal stem cells targets HDAC2 to downregulate STAT3 phosphorylation to alleviate HBx-induced ferroptosis in podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqi Chen,Xiaoqian Yang,Moxuan Feng,Yani Yu,Yongzheng Hu,Wei Jiang

Cellular and molecular gastroenterology and hepatology 17:219-235 PubMed37879404

2023

Oncostatin M Induces IFITM1 Expression to Inhibit Hepatitis B Virus Replication Via JAK-STAT Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchen Ye,Ya Fu,Caorui Lin,Ye Shen,Qingqing Yu,Xiaobao Yao,Qunfang Huang,Can Liu,Yongbin Zeng,Tianbin Chen,Songhang Wu,Zhen Xun,Qishui Ou

Journal of translational medicine 21:625 PubMed37715221

2023

Arginine methylation of HSPA8 by PRMT9 inhibits ferroptosis to accelerate hepatitis B virus-associated hepatocellular carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wensheng Deng,Jiaoyu Ai,Wanlin Zhang,Zhenyu Zhou,Muqi Li,Likun Yan,Lidong Zhang,Zongjing Huang,Ziyi Wu,Junhua Ai,Hai Jiang

JHEP reports : innovation in hepatology 5:100849 PubMed37701334

2023

JMJD2D stabilises and cooperates with HBx protein to promote HBV transcription and replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Kong,Zuofeng Liu,Ruyi Zhang,Fu'an Xie,Rubing Liang,Yong Zhang,Lingling Yu,Wensheng Yang,Xi Li,Qiang Chen,Bei Li,Yilin Hong,Ming Li,Xiaogang Xia,Lingwei Gu,Lijuan Fu,Xiaohua Li,Ye Shen,Ting Wu,Chundong Yu,Wengang Li

Virus research 329:199094 PubMed36933835

2023

A novel anti-HBV agent, E-CFCP, restores Hepatitis B virus (HBV)-induced senescence-associated cellular marker perturbation in human hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Takamatsu,Sanae Hayashi,Hiroki Kumamoto,Shuhei Imoto,Yasuhito Tanaka,Hiroaki Mitsuya,Nobuyo Higashi-Kuwata

Emerging microbes & infections 11:2486-2501 PubMed36102940

2022

Compartmentalisation of Hepatitis B virus X gene evolution in hepatocellular carcinoma microenvironment and the genotype-phenotype correlation of tumorigenicity in HBV-related patients with hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ya Fu,Fengling Fang,Hongyan Guo,Xialin Xiao,Yuhai Hu,Yongbin Zeng,Tianbin Chen,Songhang Wu,Ni Lin,Jinlan Huang,Ling Jiang,Qishui Ou,Can Liu

Infection and drug resistance 15:961-973 PubMed35299854

2022

Inhibition of Protease Activated Receptor 2 Attenuates HBx-Induced Inflammation and Mitochondria Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Yonggang Li,Shuhua Li,Hongwei Li,Ling Liu,Yao Xu

Journal of immunology (Baltimore, Md. : 1950) 208:672-684 PubMed35022275

2022

Hepatitis B and Hepatitis C Virus Infection Promote Liver Fibrogenesis through a TGF-β1-Induced OCT4/Nanog Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Li,Xiaoqiong Duan,Chuanlong Zhu,Xiao Liu,Andre J Jeyarajan,Min Xu,Zeng Tu,Qiuju Sheng,Dong Chen,Chuanwu Zhu,Tuo Shao,Zhimeng Cheng,Shadi Salloum,Esperance A Schaefer,Annie J Kruger,Jacinta A Holmes,Raymond T Chung,Wenyu Lin

Experimental and therapeutic medicine 22:1211 PubMed34584556

2021

HBxAg promotes HBV replication and EGFR activation in human placental trophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yayun Lin,Yan Liu,Dongping Xu,Fanfan Guo,Wentao Zhang,Yidan Zhang,Guiqin Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepatitis B Virus X antigen antibody [X36C]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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