Goat Polyclonal POLG antibody. Suitable for ELISA, WB and reacts with Hepatitis C virus samples. Cited in 1 publication.
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- PloS one 3:e23142008High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Hepatitis C virus | Tested | Expected |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Hepatitis C virus | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Hepatitis C virus | Dilution info Use at an assay dependent concentration. | Notes - |
Alternative names
Core protein, E1 protein, E2 protein, Envelope glycoprotein E2, HCV E2, NS2 protein, NS3 protease/helicase, NS4A protein, NS4B protein, NS5A protein, NS5B RNA-dependent RNA polymerase, P7 protein, Polyprotein, gp68, gp70
Recommended products
Goat Polyclonal POLG antibody. Suitable for ELISA, WB and reacts with Hepatitis C virus samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Purification technique
- Ion exchange chromatography
- Concentration
- Purification notes
>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Hepatitis C virus (HCV) envelope protein E2 sometimes called HCV E2 glycoprotein plays a mechanical role in viral entry into host cells. This protein forms part of the viral envelope which is expressed on the surface of the hepatitis C virus. HCV E2 is about 70 kilodaltons in mass and interacts with specific host cell receptors to mediate viral attachment and entry. It is important in establishing infection as it facilitates the fusion of the viral and host cell membranes.
- Biological function summary
The hepatitis C virus E2 glycoprotein interacts with host cell receptors such as CD81 and scavenger receptor class B type 1 (SR-B1). These interactions are necessary for virus entry and infectivity. It does not function alone but as part of a larger complex working alongside the E1 protein to mediate these processes. By forming a heterodimer with E1 the E2 protein ensures proper folding structural stability and efficient functioning within the viral lifecycle.
- Pathways
The envelope E2 protein plays a critical role in the viral entry pathway. It is an essential component of the pathway that involves initial attachment to the host cell surface. This step is followed by engagement with co-receptors and endocytosis. Proteins associated with this process include CD81 which is pivotal in facilitating the entry of HCV into hepatocytes. E2's interaction with host factors like SR-B1 further exemplifies its central role in the viral lifecycle.
- Associated diseases and disorders
Hepatitis C virus E2 is linked to hepatitis C infection which can progress to chronic liver disease and cirrhosis. Its interaction with the CD81 receptor is important for the virus's ability to infect liver cells making it a target for therapies designed to block viral entry. Chronic infection with hepatitis C is also associated with hepatocellular carcinoma with E2 likely contributing to oncogenic processes through its interactions with host cellular pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Image from Takayama H et al., PLoS One. 2008 Jun 4;3(6):e2314. doi: 10.1371/journal.pone.0002314.; Fig 5.; June 4, 2008, PLoS ONE 3(6): e2314.ELISA - Anti-Hepatitis C Virus E2 antibody (ab20044)
ELISA analysis of Hepatitis C Virus E2 binding to CD81 receptor, using ab20044 to detect Hepatitis C Virus E2 binding.
A 96-well plate was coated with 100 ng of rho-1D4 antibody. Following overnight incubation at 4°C, the unbound antibody was washed away. The wells were blocked with blocking solution for 2 hours. 100 ng of purified CD81 was added to the wells along with E2 glycoprotein (0.4 to 20 nM) and incubated for 2 hours. ab20044 anti-Hepatitis C Virus E2 antibody (1/2000 dilution) and secondary antibody with alkaline phosphatase conjugate (1/1000 dilution) were added and incubated for 2 hours. Absorbance was measured at 495 nm by a plate-reader.
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