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Mouse Monoclonal Hepatitis C Virus NS3 antibody. Suitable for ELISA, WB, IHC-P and reacts with Hepatitis C virus samples. Cited in 38 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (AB13830), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (AB13830), expandable thumbnail
  • Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (AB13830), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBIHC-P
Hepatitis C virus
Expected
Tested
Tested

Expected
Expected

Species

Hepatitis C virus

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Hepatitis C virus

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Hepatitis C virus

Dilution info

1/50

Notes

Tested using paraffin-embedded liver sections from HCV patients.

Associated Products

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Alternative names

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Mouse Monoclonal Hepatitis C Virus NS3 antibody. Suitable for ELISA, WB, IHC-P and reacts with Hepatitis C virus samples. Cited in 38 publications.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

H23

Purification technique

Affinity purification Protein G

Specificity

Hepatitis C Virus antigen NS3 helicase domain.
Reacts very well with JFH-1 strain (genotype 2a)

Epitope

Helicase domain

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

    ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

    The negative control for above picture with Huh7 cells.

  • Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830), expandable thumbnail
    Image from Kota S et al., PLoS One. 2012;7(2):e32207. Epub 2012 Feb 28.; Fig 3.; doi:10.1371/journal.pone.0032207; February 28, 2012, PLoS ONE 7(2): e32207.

    Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

    Representative Western Blot detecting Hepatitis C Virus NS3 with ab13830.

    SL209-biotin was immobilized on streptavidin agarose beads and incubated with lysates of Huh-7.5 cells infected with HCV J6/JFH-1. Retained proteins were examined by western blot.

    All lanes: Western blot - Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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