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Mouse Recombinant Monoclonal POLG antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Hepatitis C virus, Transfected cell line - Hepatitis C virus samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521), expandable thumbnail
  • Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (AB322521), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Hepatitis C virus
Predicted
Predicted
Transfected cell line - Hepatitis C virus
Not recommended
Tested
Transfected cell lysate - Hepatitis C virus
Tested
Not recommended

Tested
Tested

Species
Transfected cell lysate - Hepatitis C virus
Dilution info
1/1000
Notes

-

Predicted
Predicted

Species
Hepatitis C virus
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Hepatitis C virus
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Hepatitis C virus
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Hepatitis C virus
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Hepatitis C virus
Dilution info
-
Notes

-

Target data

Alternative names

HCV NS5B, P68, RNA dependent RNA polymerase, RNA directed RNA polymerase

Mouse Recombinant Monoclonal POLG antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate - Hepatitis C virus, Transfected cell line - Hepatitis C virus samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
1A4
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Hepatitis C Virus region NS5B with ab322521 at 1/2000 (0.47 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-His-tag®(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

  • Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521), expandable thumbnail

    Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-Hepatitis C Virus region NS5B antibody [1A4] (ab322521) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Hepatitis C virus genotype 2a (isolate JFH-1) (HCV) region NS5B expression vector containing a myc-his-tag, whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Observed band size: 65 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com