JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308336

Anti-Hepatitis D virus Large delta protein antibody [HL1051]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Hepatitis D Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Hepatitis D virus samples. Immunogen corresponding to Synthetic Peptide within Hepatitis delta virus (ISOLATE D380) Large delta antigen.

View Alternative Names

Large delta antigen, L-HDAg, p27

2 Images
Western blot - Anti-Hepatitis D virus Large delta protein antibody [HL1051] (AB308336)
  • WB

Supplier Data

Western blot - Anti-Hepatitis D virus Large delta protein antibody [HL1051] (AB308336)

Non-transfected (–) and transfected (+) Huh-7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis D virus Large delta protein antibody [HL1051] (ab308336) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Lanes 1 - 3:

Western blot - Anti-Hepatitis D virus Large delta protein antibody [HL1051] (ab308336) at 1/5000 dilution

Lanes 1 and 3:

Non-transfected (–) Huh-7 whole cell extracts at 30 µg

Lane 2:

Transfected (+) Huh-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta protein antibody [HL1051] (AB308336)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta protein antibody [HL1051] (AB308336)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Hepatitis D virus Large delta protein using ab308336 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1051

Isotype

IgG

Carrier free

Yes

Reacts with

Hepatitis D virus

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Hepatitis delta virus (ISOLATE D380) Large delta antigen. The exact immunogen used to generate this antibody is proprietary information.

P29996

style
left-column

Complementary Products

Proteins & Peptides

AB49050

Recombinant Hepatitis D Virus protein

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis D virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Hepatitis D virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis D Virus (HDV) also known as delta virus is a small circular RNA virus. It is unique among viruses because it requires the presence of Hepatitis B Virus (HBV) for its replication and infection cycle. The delta protein encoded by the HDV RNA genome has an important role in its replication. The delta protein typically has a mass of approximately 24 kDa and is expressed specifically in the liver cells of infected individuals. Due to its dependence on HBV HDV is classified within the family of Deltaviridae.
Biological function summary

The delta protein interacts with host cellular machinery to facilitate HDV replication. It is part of a ribonucleoprotein complex that consists of the viral RNA and the delta protein itself. The complex enables the replication of the viral genome and packaging of the mature virus. The HDV RNA acts as both a template for replication and a ribozyme allowing it to self-cleave and process into the mature genomic RNA. The delta protein in its large form is essential for encapsidation with the HBV surface antigens completing the assembly of infectious virus particles.

Pathways

Hepatitis D virus follows a unique co-infective pathway relying heavily on the HBV lifecycle. The interaction between the HDV delta protein and HBV surface antigens particularly the large HBV surface antigen plays an important role in the assembly and secretion of new virions. HDV actively manipulates the host cellular pathways to facilitate its replication and requires the host's RNA polymerase II for synthesizing its RNA genome. The HBV-associated pathogenesis further accentuates liver damage caused by co-infection.

HDV infection significantly contributes to severe liver diseases including fulminant hepatitis and liver cirrhosis and it increases the risk of hepatocellular carcinoma in affected patients. The combined infection of HDV and HBV leads to a more aggressive disease course and the presence of HBV proteins especially the surface antigen is necessary for HDV propagation. Research into hepatitis D antibodies targets these viral proteins to develop novel therapeutic strategies and prevent liver disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.
See full target information Large delta antigen
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com