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AB308391

Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055]

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Rabbit Recombinant Monoclonal Hepatitis D Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Hepatitis D virus samples.

View Alternative Names

Large delta antigen, L-HDAg, p27

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Hepatitis D virus Large delta + Small delta protein using ab308391 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391)
  • WB

Supplier Data

Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391)

Non-transfected (–) and transfected (+) Huh-7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis D virus Large data + Small delta protein antibody [HL1055] (ab308391) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391) at 1/5000 dilution

Lane 1:

Non-transfected (–) Huh-7 whole cell extracts at 30 µg

Lanes 2 - 3:

Transfected (+) Huh-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1055

Isotype

IgG

Carrier free

Yes

Reacts with

Hepatitis D virus

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis D virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Hepatitis D virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Hepatitis D Small delta antigen (SDAg) and Large delta antigen (LDAg) are essential proteins of the Hepatitis D virus (HDV) known for their involvement in the virus's replication and pathogenesis. The Small delta antigen weighs approximately 24 kDa and the Large delta antigen is about 195 kDa. These proteins are expressed in the cytoplasm and nucleus of infected liver cells where they perform distinct yet interconnected roles in the life cycle of HDV. SDAg facilitates viral replication while LDAg is important for virion assembly.
Biological function summary

The Hepatitis D antigens Small and Large are part of a dynamic complex that plays a significant role in HDV infection. The Small delta antigen enhances the replication of viral RNA by interacting with the cellular machinery effectively hijacking the host cell's resources. In contrast the Large delta antigen mediates encapsidation processes that lead to the formation of new virions. This functional dichotomy within the antigens highlights their unique contributions to HDV persistence in the host organism.

Pathways

Hepatitis D Small and Large delta antigens interface with cellular pathways that are critical for viral survival and propagation. The interferon signaling pathway is notably affected by these antigens as they manipulate host responses to favor HDV replication. Another impacted pathway includes the RNA interference pathway which is modulated by interactions between delta antigens and cellular proteins like Dicer. These interactions indicate the sophisticated strategies HDV employs to leverage host cellular pathways to its advantage.

The Hepatitis D Small and Large delta antigens are directly tied to Hepatitis D infection a severe form of viral hepatitis that can exacerbate the condition in individuals with concurrent Hepatitis B infection. The presence of LDAg correlates with disease progression and liver damage as it relates to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. These delta antigens also interact with the Hepatitis B surface antigen facilitating virion assembly and enabling the HDV to propagate alongside HBV indicating their contribution to co-infection dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.
See full target information Large delta antigen
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com