Skip to main content

Rabbit Recombinant Monoclonal Hepatitis D Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (AB308391), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Transfected cell line
Tested
Tested

Tested
Tested

Species
Transfected cell line
Dilution info
1/500
Notes

-

Tested
Tested

Species
Transfected cell line
Dilution info
1/5000
Notes

-

Target data

Function

Following virus entry into host cell, provides nuclear import of HDV RNPs thanks to its nuclear localization signal. Needs co-infection with hepatitis B virus to provide surface proteins, otherwise there is no packaging or budding. Packages the HDV ribonucleoprotein in hepatitis B virus empty particles. Interacts with both HDV genomic RNA and cytoplasmic tail of HBsAg. May inhibit viral RNA replication.

Additional Targets

Hepatitis D Small delta antigen

Alternative names

Large delta antigen, L-HDAg, p27

Rabbit Recombinant Monoclonal Hepatitis D Virus antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
HL1055
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Hepatitis D Small delta antigen (SDAg) and Large delta antigen (LDAg) are essential proteins of the Hepatitis D virus (HDV) known for their involvement in the virus's replication and pathogenesis. The Small delta antigen weighs approximately 24 kDa and the Large delta antigen is about 195 kDa. These proteins are expressed in the cytoplasm and nucleus of infected liver cells where they perform distinct yet interconnected roles in the life cycle of HDV. SDAg facilitates viral replication while LDAg is important for virion assembly.

Biological function summary

The Hepatitis D antigens Small and Large are part of a dynamic complex that plays a significant role in HDV infection. The Small delta antigen enhances the replication of viral RNA by interacting with the cellular machinery effectively hijacking the host cell's resources. In contrast the Large delta antigen mediates encapsidation processes that lead to the formation of new virions. This functional dichotomy within the antigens highlights their unique contributions to HDV persistence in the host organism.

Pathways

Hepatitis D Small and Large delta antigens interface with cellular pathways that are critical for viral survival and propagation. The interferon signaling pathway is notably affected by these antigens as they manipulate host responses to favor HDV replication. Another impacted pathway includes the RNA interference pathway which is modulated by interactions between delta antigens and cellular proteins like Dicer. These interactions indicate the sophisticated strategies HDV employs to leverage host cellular pathways to its advantage.

Associated diseases and disorders

The Hepatitis D Small and Large delta antigens are directly tied to Hepatitis D infection a severe form of viral hepatitis that can exacerbate the condition in individuals with concurrent Hepatitis B infection. The presence of LDAg correlates with disease progression and liver damage as it relates to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. These delta antigens also interact with the Hepatitis B surface antigen facilitating virion assembly and enabling the HDV to propagate alongside HBV indicating their contribution to co-infection dynamics.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391), expandable thumbnail

    Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391)

    Non-transfected (–) and transfected (+) Huh-7 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis D virus Large data + Small delta protein antibody [HL1055] (ab308391) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391) at 1/5000 dilution

    Lane 1: Non-transfected (–) Huh-7 whole cell extracts at 30 µg

    Lanes 2 - 3: Transfected (+) Huh-7 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis D virus Large delta + Small delta protein antibody [HL1055] (ab308391)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Hepatitis D virus Large delta + Small delta protein using ab308391 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com