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AB233244

Anti-Hepatitis E Virus antibody [5F3]

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(4 Publications)

Mouse Monoclonal Hepatitis E Virus antibody. Suitable for ELISA, ICC/IF, WB and reacts with Hepatitis E virus type 3 samples. Cited in 4 publications.

View Alternative Names

HEV

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis E Virus antibody [5F3] (AB233244)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

HEV-3-infected cells were fixed with acetone-methanol (1 : 1; 20 °C) and blocked with 1% FCS, then incubated with ab233244 at 1/50 dilution for 1 h at 37°C. FITC-conjugated anti-mouse IgG at 1/100 dilution was used as the secondary antibody. Cell nuclei counterstained with DAPI (Blue).

Western blot - Anti-Hepatitis E Virus antibody [5F3] (AB233244)
  • WB

Supplier Data

Western blot - Anti-Hepatitis E Virus antibody [5F3] (AB233244)

M - PageRuler Prestained Protein Ladder

All lanes:

Western blot - Anti-Hepatitis E Virus antibody [5F3] (ab233244) at 1/1000 dilution

Lane 1:

Rat HEV capsid protein at 0.5 µg

Lane 2:

HEV genotype 3 at 0.5 µg

Lane 3:

Human parvovirus 4 VP2 protein at 0.5 µg

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5F3

Isotype

IgG2b

Carrier free

No

Reacts with

Hepatitis E virus type 3

Applications

ELISA, WB, ICC/IF

applications

Specificity

This antibody recognizes the capsid protein of the HEV genotype 3. Does not react with rat HEV capsid protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Hepatitis E virus type 3": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20 - 1/50", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepatitis E virus (HEV) is a non-enveloped RNA virus with a genome that encodes three open reading frames. The mass of the virus particle has not been explicitly determined due to its virus nature but it comprises several proteins including the capsid protein which is essential for virion assembly. HEV predominantly expresses in the liver as this organ serves as the primary site for its replication. Commonly researchers refer to hepatitis E virus by its abbreviation HEV in scientific literature.
Biological function summary

HEV impacts liver function through its replication within hepatocytes contributing to liver inflammation and damage. The virus does not form a complex with other viral components but relies on host cell machinery for its replication cycle. Hepatitis E antibodies including anti-HEV and HEV antibodies such as 5f3 can target the virus for immune recognition and clearance with the immune response playing an important role in controlling and eliminating the infection.

Pathways

The virus integrates into the immune signaling pathways associated with viral recognition and response. HEV interacts with innate immune pathways to subvert the host's defense mechanisms. The interferon pathway is particularly significant in moderating the viral life cycle and proteins such as interferon-stimulated genes (ISGs) become upregulated in response to HEV presence. Additionally interactions with proteins within the apoptotic pathway may contribute to virus-induced cellular responses influencing both cell survival and antiviral defense.

HEV is primarily linked to hepatitis E an acute liver disease with symptoms such as jaundice fatigue and nausea. This condition can become more severe in pregnant women leading to maternal mortality. HEV infection can also escalate into chronic hepatitis particularly in immunocompromised individuals. In chronic cases the virus associates with proteins involved in the liver fibrosis process potentially contributing to long-term liver damage and cirrhosis. Current research continues to explore the relationship between hepatitis E antibodies and the modulation of disease outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Hepatitis E Virus
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 56:1365-1372 PubMed38877781

2024

Hepatitis E virus infection upregulates ING5 expression and .

Applications

Unspecified application

Species

Unspecified reactive species

Wanqiu Zhao,Yueping Xia,Tengyuan Li,Huichan Liu,Guo Zhong,Dongxue Chen,Wenhai Yu,Yunlong Li,Fen Huang

Viruses 13: PubMed33924409

2021

Molecular Characterization and Seroprevalence of Hepatitis E Virus in Inflammatory Bowel Disease Patients and Solid Organ Transplant Recipients.

Applications

ICC/IF

Species

Human

Juozas Grigas,Maria Montoya,Evelina Simkute,Marius Buitkus,Ruta Zagrabskaite,Arnoldas Pautienius,Dainius Razukevicius,Laimas Virginijus Jonaitis,Gediminas Kiudelis,Jurgita Skieceviciene,Ruta Vaiciuniene,Asta Stankuviene,Inga Arune Bumblyte,Juozas Kupcinskas,Arunas Stankevicius

BMC veterinary research 16:95 PubMed32199460

2020

Hepatitis E genotype 3 virus isolate from wild boar is capable of replication in non-human primate and swine kidney cells and mouse neuroblastoma cells.

Applications

ICC/IF

Species

Unspecified reactive species

Juozas Grigas,Evelina Simkute,Martynas Simanavicius,Arnoldas Pautienius,Zaneta Streimikyte-Mockeliune,Dainius Razukevicius,Arunas Stankevicius

Applied microbiology and biotechnology 102:185-198 PubMed29143081

2017

Generation in yeast and antigenic characterization of hepatitis E virus capsid protein virus-like particles.

Applications

Unspecified application

Species

Unspecified reactive species

Martynas Simanavicius,Paulius Lukas Tamosiunas,Rasa Petraityte-Burneikiene,Reimar Johne,Rainer G Ulrich,Aurelija Zvirbliene,Indre Kucinskaite-Kodze
View all publications
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