Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody that is used to detect Hepcidin-25 in Western Blot, IHC-P. Suitable for Human, Rat samples.
- Over 40 publications
- Trusted since 2007
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- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229852023A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Lv,Yijia Wang,Chenxiao Zhen,Junyu Liu,Xin Chen,Gejing Zhang,Wei Yao,Huijie Guo,Yunpeng Wei,Shenghang Wang,Jiancheng Yang,Peng ShangPubMed 37249350
- PloS one 18:e02846172023Hepcidin expression in the trigeminal ganglion and the oral mucosa in an oral ulcerative mucositis rat model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuzuro Hitomi,Tomotaka Nodai,Shoichiro Kokabu,Takemi Shikayama,Misa Sago-Ito,Chihiro Nakatomi,Kiyoshi Terawaki,Yuji Omiya,Masamichi Shinoda,Kentaro OnoPubMed 37079608
- Frontiers in endocrinology 13:10488182023PTH 1-34 promoted bone formation by regulating iron metabolism in unloading-induced bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingmin Che,Weihao Ren,Xin Chen,Fang Wang,Gejing Zhang,Peng ShangPubMed 36818465
- Frontiers in immunology 13:9855382023Low circulatory Fe and Se levels with a higher IL-6/IL-10 ratio provide nutritional immunity in tuberculosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSandeep R Kaushik,Sukanya Sahu,Hritusree Guha,Sourav Saha,Ranjit Das,Rukuwe-U Kupa,Wetetsho Kapfo,Trinayan Deka,Rumi Basumatary,Asunu Thong,Arunabha Dasgupta,Bidhan Goswami,Amit Kumar Pandey,Lahari Saikia,Vinotsole Khamo,Anjan Das,Ranjan Kumar NandaPubMed 36713405
- Mediators of inflammation 2022:69434382022Electroacupuncture Reduces Cerebral Hemorrhage Injury in Rats by Improving Cerebral Iron Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuxin Chen,Wenjing Song,Yihe Tang,Yizhou Tang,Yuying Kang,Luwen ZhuPubMed 36016663
- Hepatology communications 6:2914-29242022Loss of SLC46A1 decreases tumor iron content in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyao Wang,Huiwen Wu,Jianxin Yang,Min Li,Changquan Ling,Zelong Gao,Hongtao Lu,Hui Shen,Yuxiao TangPubMed 35811443
- BMC oral health 22:2632022Role of NOD2 and hepcidin in inflammatory periapical periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Hu,Marie Aimee Dusenge,Qin Ye,Ya-Qiong Zhao,Li Tan,Yao Feng,Jie Zhao,Zheng-Rong Gao,Shao-Hui Zhang,Yun Chen,Ying-Hui Zhou,Yue Guo,Yun-Zhi FengPubMed 35764993
- Frontiers in microbiology 13:8631332022Novel Secreted Peptides From var. With Immunomodulatory Effects That Enhance Fungal Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSameh S M Soliman,Eman M El-Labbad,Ameera Abu-Qiyas,Bahgat Fayed,Alshaimaa M Hamoda,Ahmed M Al-Rawi,Salam Dakalbab,Abdel-Nasser A El-Shorbagi,Mawieh Hamad,Ashraf S Ibrahim,Mohammad G MohammadPubMed 35387075
- International journal of molecular sciences 23:2022Interplay of Ferritin Accumulation and Ferroportin Loss in Ageing Brain: Implication for Protein Aggregation in Down Syndrome Dementia, Alzheimer's, and Parkinson's Diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnimesh Alexander Raha,Anwesha Biswas,James Henderson,Subhojit Chakraborty,Anthony Holland,Robert P Friedland,Elizabeta Mukaetova-Ladinska,Shahid Zaman,Ruma Raha-ChowdhuryPubMed 35162984
- Oxidative medicine and cellular longevity 2021:98007942021Hepcidin Promoted Ferroptosis through Iron Metabolism which Is Associated with DMT1 Signaling Activation in Early Brain Injury following Subarachnoid Hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxia Zhang,Robert Ostrowski,Dengzhi Jiang,Qing Zhao,Yidan Liang,Xudong Che,Jun Zhao,Xiang Xiang,Wang Qin,Zhaohui HePubMed 34987706
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/100.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed:22682227, PubMed:29237594, PubMed:32814342). Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005). Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11034317, PubMed:11113131).
Alternative names
HEPC, LEAP1, UNQ487/PRO1003, HAMP, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, LEAP-1, PLTR
Recommended products
Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody that is used to detect Hepcidin-25 in Western Blot, IHC-P. Suitable for Human, Rat samples.
- Over 40 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all species. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Rat samples.
What is the molecular weight of Hepcidin-25?
Anti-Hepcidin-25 (ab30760) specifically detects a band for Hepcidin-25 (UniProt: P81172) at a molecular weight of 3kDa.
Trusted by the scientific community
Anti-Hepcidin-25 (ab30760) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-Hepcidin-25 (ab30760) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.
- Biological function summary
Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.
- Pathways
Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.
- Associated diseases and disorders
Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-Hepcidin-25 antibody (ab30760)
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.
All lanes: Western blot - Anti-Hepcidin-25 antibody (ab30760) at 1 µg/mL
All lanes: HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 32 kDa
Exposure time: 3min
This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)
Hepcidin-25 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Hepcidin-25 antibody
IHC image of Hepcidin-25 staining in Human Hepatocellular Carcinoma (HCC) formalin fixed paraffin embedded tissue section*.
Performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30760 at 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
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