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Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody that is used to detect Hepcidin-25 in Western Blot, IHC-P. Suitable for Human, Rat samples.



- Over 40 publications

- Trusted since 2007


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760), expandable thumbnail
  • Western blot - Anti-Hepcidin-25 antibody (AB30760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form
Liquid
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Expected
Expected

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10.00000 - 1/100.00000
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Mouse
Dilution info
-
Notes

-

Associated Products

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Target data

Function

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed:22682227, PubMed:29237594, PubMed:32814342). Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005). Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11034317, PubMed:11113131).

Alternative names

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Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody that is used to detect Hepcidin-25 in Western Blot, IHC-P. Suitable for Human, Rat samples.



- Over 40 publications

- Trusted since 2007

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique
Affinity purification Immunogen
Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all species. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Rat samples.

What is the molecular weight of Hepcidin-25?


Anti-Hepcidin-25 (ab30760) specifically detects a band for Hepcidin-25 (UniProt: P81172) at a molecular weight of 3kDa.

Trusted by the scientific community


Anti-Hepcidin-25 (ab30760) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists


Anti-Hepcidin-25 (ab30760) has over 5 independent reviews from customers.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.

Biological function summary

Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.

Pathways

Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.

Associated diseases and disorders

Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)

    IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Hepcidin-25 antibody (ab30760), expandable thumbnail

    Western blot - Anti-Hepcidin-25 antibody (ab30760)

    ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.

    All lanes: Western blot - Anti-Hepcidin-25 antibody (ab30760) at 1 µg/mL

    All lanes: HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 9 kDa

    Observed band size: 32 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760), expandable thumbnail
    This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)

    Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (ab30760)

    Hepcidin-25 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Hepcidin-25 antibody

    IHC image of Hepcidin-25 staining in Human Hepatocellular Carcinoma (HCC) formalin fixed paraffin embedded tissue section*.

     Performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30760 at 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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