AB30760

Anti-Hepcidin-25 antibody

Name: Anti-Hepcidin-25 antibody (ab30760) | Abcam
Brand: Abcam Limited
SKU: AB30760
Price: 180 USD
Availability: InStock
Rating: 4 (9 reviews)

BOND RX™ Validated
What is this?

(9 Reviews)

(54 Publications)

Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody detecting Hepcidin-25 in Western Blot, IHC-P. Suitable for Human, Rat.

- Over 40 publications
- Trusted since 2007

View Alternative Names

HEPC, LEAP1, UNQ487/PRO1003, HAMP, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, LEAP-1, PLTR

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

IHC image of Hepcidin-25 staining in Human normal liver formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

IHC image of Hepcidin-25 staining in Human Hepatocellular Carcinoma (HCC) formalin fixed paraffin embedded tissue section*.

Performed on a Leica BOND^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20mins. The section was then incubated with ab30760 at 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Collaborator

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

Hepcidin-25 expression in rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760). Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.

This image is courtesy of Ruma Raha-Chowdhury (Cambridge University)

Project

Western blot - Anti-Hepcidin-25 antibody (AB30760)

ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.

All lanes:

Western blot - Anti-Hepcidin-25 antibody (ab30760) at 1 µg/mL

All lanes:

HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 9 kDa

Observed band size: 32 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all species. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10 - 1/100", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/10 - 1/100", "WB-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Hepcidin-25 antibody (ab30760) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Rat samples.

What is the molecular weight of Hepcidin-25?
Anti-Hepcidin-25 (ab30760) specifically detects a band for Hepcidin-25 (UniProt: P81172) at a molecular weight of 3kDa.

Trusted by the scientific community
Anti-Hepcidin-25 (ab30760) was first used in a scientific publication in 2007 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Hepcidin-25 (ab30760) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.

Biological function summary

Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.

Pathways

Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.

Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed : 22682227, PubMed : 29237594, PubMed : 32814342). Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed : 22306005).. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed : 11034317, PubMed : 11113131).

See full target information HAMP

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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

Journal of dental sciences 20:1562-1570 PubMed40654472

2025

Hepcidin promotes oral ulcer healing via amphiregulin production in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Naoto Taguchi,Suzuro Hitomi,Hitoshi Sato,Yoshinori Hayashi,Ikuko Shibuta,Koichi Iwata,Tatsuo Shirota,Masamichi Shinoda

International journal of molecular sciences 26: PubMed39941155

2025

Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Xie,Linyue Chang,Xinyue Zhu,Fengbei Gong,Linlin Che,Rujun Zhang,Lixin Wang,Chenyuan Gong,Cheng Fang,Chao Yao,Dan Hu,Weimin Zhao,Yufu Zhou,Shiguo Zhu

Journal of clinical biochemistry and nutrition 75:190-196 PubMed39583972

2024

Effect of cerium oxide on iron metabolism in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Kanome,Shunki Ohtomo,Masaharu Nakatsu,Masahiro Kohno,Koji Fukui

FASEB bioAdvances 6:263-275 PubMed39114446

2024

Social defeat stress impairs systemic iron metabolism by activating the hepcidin-ferroportin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Emiko Kasahara,Ayumi Nakamura,Kenki Morimoto,Shiho Ito,Mika Hori,Atsuo Sekiyama

Nature communications 15:6718 PubMed39112467

2024

Skin hepcidin initiates psoriasiform skin inflammation via Fe-driven hyperproliferation and neutrophil recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Elise Abboud,Doha Chrayteh,Nadia Boussetta,Héloise Dalle,Mariangela Malerba,Ting-Di Wu,Morgane Le Gall,Olivier Reelfs,Charareh Pourzand,Mark Mellett,Florence Assan,Hervé Bachelez,Joël Poupon,Selim Aractingi,Sophie Vaulont,Pierre Sohier,Bénédicte Oules,Zoubida Karim,Carole Peyssonnaux

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:4572-4582 PubMed38899570

2024

tPA supplementation preserves neurovascular and cognitive function in Tg2576 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Uekawa,Antoine Anfray,Sung Ji Ahn,Nicole Casey,James Seo,Ping Zhou,Costantino Iadecola,Laibaik Park

Aging 16:8524-8540 PubMed38787358

2024

HAMP predicts a pivotal role in modulating the malignant behaviors of non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifeng Li,Jinglei Liu,Ping Wang,Boyu Zhang,Guanghui He,Liwei Yang

Scientific reports 14:3986 PubMed38368463

2024

Bone marrow stromal cell-derived hepcidin has antimicrobial and immunomodulatory activities.

Applications

Unspecified application

Species

Unspecified reactive species

Miklós Krepuska,Balázs Mayer,Lynn Vitale-Cross,Vamsee D Myneni,Michael K Boyajian,Krisztián Németh,Ildikó Szalayova,Ted Cho,Ian McClain-Caldwell,Aaron D Gingerich,Huiling Han,Mark Westerman,Balázs Rada,Éva Mezey

Cell death & disease 15:49 PubMed38218852

2024

Transmembrane serine protease 6, a novel target for inhibition of neuronal tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Zuo,Jiawei Bai,Huiyuan Bai,Siyu Tian,Hongtao Sun,Zhenhua Shi,Peng Yu,Guofen Gao,Yuan Li,Yan-Zhong Chang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22985 PubMed37249350

2023

A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Huanhuan Lv,Yijia Wang,Chenxiao Zhen,Junyu Liu,Xin Chen,Gejing Zhang,Wei Yao,Huijie Guo,Yunpeng Wei,Shenghang Wang,Jiancheng Yang,Peng Shang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepcidin-25 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin-25 antibody (AB30760)

Western blot - Anti-Hepcidin-25 antibody (AB30760)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (54)

Product promise