AB187778

Anti-Hepcidin-25 antibody [EPR18074]

Name: Anti-Hepcidin-25 antibody [EPR18074] (ab187778) | Abcam
Brand: Abcam Limited
SKU: AB187778
Price: 290 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal Hepcidin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 3 publications.

View Alternative Names

HEPC, LEAP1, UNQ487/PRO1003, HAMP, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, LEAP-1, PLTR

3 Images

Supplier Data

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 1% NFDM/TBST.

All lanes:

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human serum at 20 µg

Lane 3:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

Predicted band size: 9 kDa

Observed band size: 3 kDa

false

Supplier Data

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Blocking/Dilution buffer : 5% NFDM/TBST.

In house recombinant Human Hepcidin is a His-tagged fragment protein (aa25-84).

All lanes:

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution

All lanes:

Human Hepcidin fragment protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 9 kDa

Observed band size: 17 kDa

false

Exposure time: 1s

Lab

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Blocking buffer : 5% NFDM/TBST. Dilution buffer : 1% NFDM/TBST

All lanes:

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

Lane 1:

Serum from rat treated with LPS at 30 µg

Lane 2:

Serum from rat treated with LPS at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

false

Exposure time: 3min

Carrier free

Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18074

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.

Biological function summary

Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.

Pathways

Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.

Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed : 22682227, PubMed : 29237594, PubMed : 32814342). Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed : 22306005).. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed : 11034317, PubMed : 11113131).

See full target information HAMP

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International immunopharmacology 69:337-346 PubMed30776642

2019

Anti-hyperalgesia effect of nanchelating based nano particle, RAc1, can be mediated via liver hepcidin expression modulation during persistent inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Vida Nazemian,Somayeh Kalanaky,Homa Manaheji,Etrat Houshmandi,Mola Mohammadi,Jalal Zaringhalam,Somayeh Mirjafai

Experimental and therapeutic medicine 16:1963-1967 PubMed30186425

2018

Reduced hepcidin level features osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Liu,Caihua Liu,Weifeng Zhong,Min Song,Shouqin Du,Jianli Su

Biochimica et biophysica acta. Molecular basis of 1863:1568-1574 PubMed28254494

2017

Sodium restriction modulates innate immunity and prevents cardiac remodeling in a rat model of metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Bernard Jover,Christelle Reynes,Caroline Rugale,Cyril Reboul,Laura Jeanson,Michel Tournier,Anne Dominique Lajoix,Caroline Desmetz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hepcidin-25 antibody [EPR18074]

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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