Rabbit Recombinant Monoclonal Hepcidin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | |
---|---|
Human | Tested |
Rat | Tested |
Recombinant fragment - Human | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
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Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed:22682227, PubMed:29237594, PubMed:32814342). Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11034317, PubMed:11113131).
HEPC, LEAP1, UNQ487/PRO1003, UNQ487/PRO1003, HEPC, LEAP1, HAMP, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, LEAP-1, PLTR
Rabbit Recombinant Monoclonal Hepcidin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 3 publications.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR18074
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.
Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.
Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.
Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Blocking/Dilution buffer: 5% NFDM/TBST.
In house recombinant Human Hepcidin is a His-tagged fragment protein (aa25-84).
All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution
All lanes: Human Hepcidin fragment protein at 0.01 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 9 kDa
Observed band size: 17 kDa
Exposure time: 1s
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST.
All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1: Human plasma at 20 µg
Lane 2: Human serum at 20 µg
Lane 3: Human liver lysate at 20 µg
All lanes: Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Predicted band size: 9 kDa
Observed band size: 3 kDa
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 1% NFDM/TBST
All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution
Lane 1: Serum from rat treated with LPS at 30 µg
Lane 2: Serum from rat treated with LPS at 10 µg
All lanes: Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution
Exposure time: 3min
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