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Rabbit Recombinant Monoclonal Hepcidin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778), expandable thumbnail
  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778), expandable thumbnail
  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] (AB187778), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Rat
Tested
Recombinant fragment - Human
Tested

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed:22682227, PubMed:29237594, PubMed:32814342). Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed:22306005).Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed:11034317, PubMed:11113131).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Hepcidin antibody. Suitable for WB and reacts with Recombinant fragment - Human, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18074

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.

Biological function summary

Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.

Pathways

Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.

Associated diseases and disorders

Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778), expandable thumbnail

    Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    In house recombinant Human Hepcidin is a His-tagged fragment protein (aa25-84).

    All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/5000 dilution

    All lanes: Human Hepcidin fragment protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 9 kDa

    Observed band size: 17 kDa

    Exposure time: 1s

  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778), expandable thumbnail

    Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST.

    All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

    Lane 1: Human plasma at 20 µg

    Lane 2: Human serum at 20 µg

    Lane 3: Human liver lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

    Predicted band size: 9 kDa

    Observed band size: 3 kDa

  • Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778), expandable thumbnail

    Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778)

    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 1% NFDM/TBST

    All lanes: Western blot - Anti-Hepcidin-25 antibody [EPR18074] (ab187778) at 1/1000 dilution

    Lane 1: Serum from rat treated with LPS at 30 µg

    Lane 2: Serum from rat treated with LPS at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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