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AB238974

Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal Hepcidin antibody. Carrier free. Suitable for WB and reacts with Human, Rat samples. Cited in 2 publications.

View Alternative Names

HEPC, LEAP1, UNQ487/PRO1003, HAMP, Hepcidin, Liver-expressed antimicrobial peptide 1, Putative liver tumor regressor, LEAP-1, PLTR

2 Images
Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (AB238974)
  • WB

Supplier Data

Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (AB238974)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 1% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187778).

All lanes:

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (<a href='/en-us/products/primary-antibodies/hepcidin-25-antibody-epr18074-ab187778'>ab187778</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human serum at 20 µg

Lane 3:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

Predicted band size: 9 kDa

Observed band size: 3 kDa

false

Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (AB238974)
  • WB

Lab

Western blot - Anti-Hepcidin-25 antibody [EPR18074] - BSA and Azide free (AB238974)

Blocking buffer : 5% NFDM/TBST. Dilution buffer : 1% NFDM/TBST

All lanes:

Western blot - Anti-Hepcidin-25 antibody [EPR18074] (<a href='/en-us/products/primary-antibodies/hepcidin-25-antibody-epr18074-ab187778'>ab187778</a>) at 1/1000 dilution

Lane 1:

Serum from rat treated with LPS at 30 µg

Lane 2:

Serum from rat treated with LPS at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP), preadsorbed at 1/50000 dilution

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18074

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab238974 is the carrier-free version of ab187778.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin-25 also known simply as hepcidin is a 25-amino acid peptide hormone with a molecular weight of approximately 2.8 kDa (kilodaltons). This peptide is primarily produced in the liver and circulates in the bloodstream. Mechanically hepcidin regulates iron homeostasis by binding to the iron export channel ferroportin on cell surfaces like enterocytes in the gut and macrophages. By binding ferroportin hepcidin induces its internalization and degradation reducing iron efflux into the plasma.
Biological function summary

Hepcidin serves as the master regulator of iron metabolism. Unlike many peptides it does not form part of a larger protein complex. Its activity ensures adequate iron levels for erythropoiesis while preventing toxic iron overload. It affects not only the liver but also distant tissues like the bone marrow and spleen. Hepcidin inhibits iron absorption in the intestine and controls iron release from macrophages and hepatic stores maintaining systemic iron concentration within a narrow range.

Pathways

Hepcidin operates within the complex network of iron metabolism and erythropoiesis pathways. It is particularly important in the liver hepcidin signaling pathway and the systemic iron regulation pathway. Hepcidin interacts closely with proteins like ferroportin its receptor and target as well as other iron transporters which ensures efficient iron allocation in the body. It also connects to other regulatory molecules like transferrin receptor and ferritin key players in iron transport and storage.

Hepcidin has significant implications for conditions like anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease hepcidin production increases abnormally trapping iron within cells and reducing its serum levels therefore decreasing hemoglobin synthesis despite adequate iron stores. In hereditary hemochromatosis genetic mutations lead to low hepcidin levels causing excess iron absorption and progressive iron overload in tissues. The connection of hepcidin to proteins like ferroportin and transferrin receptor helps elucidate its role in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma (PubMed : 22682227, PubMed : 29237594, PubMed : 32814342). Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes (PubMed : 22306005).. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa (PubMed : 11034317, PubMed : 11113131).
See full target information HAMP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular histology 56:302 PubMed40921925

2025

Tongxinluo alleviates myocardial ischemia-reperfusion injury by inhibiting the pyroptosis of endothelial cells via the NLRP3/Caspase-1/GSDMD signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Wu,Yun-Long Hou,Tong-Xing Wang,Li-Ping Chang,Hong-Ru Zhou,Ming-Ye Wang,Yi-Ling Wu

Frontiers in oncology 15:1579843 PubMed40661777

2025

Analysis of the molecular subtypes and prognostic models of anoikis-related genes in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Shen,Kang Hou,Jifeng Zhang,Xiaodong Li
View all publications
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Product promise

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