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AB317523

Anti-Hepcidin antibody [EPR28012-24] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Hepcidin antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

View Alternative Names

Hepc, Hamp, Hepcidin

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Sandwich ELISA - Anti-Hepcidin antibody [EPR28012-24] - BSA and Azide free (Detector) (AB317523)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Hepcidin antibody [EPR28012-24] - BSA and Azide free (Detector) (AB317523)

Sandwich ELISA of ab317521 with the capture antibody ab317522 dilution at 2 ug/mL and detector antibody ab317523 dilution at 0.5 ug/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28012-24

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has cross reactivity with full length HAMP.

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Reactivity data

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Product details

ab317523 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat Hepcidin. The recommended pair for sandwich ELISA is:

Capture: ab317522, Rat Hepcidin Capture Antibody (unconjugated)

Detector: ab317523, Rat Hepcidin Detector Antibody (unconjugated)

The reference range value is 15.63-500 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Hepcidin also known as Liver-Expressed Antimicrobial Peptide (LEAP) or HEPC is an important regulator of iron homeostasis functioning as a hormone. It consists of a small peptide typically around 25 amino acids in length with a molecular mass of approximately 2.8 kDa. Hepcidin is produced predominantly in the liver and is secreted into the bloodstream where it directly binds to the iron export protein ferroportin. This binding induces ferroportin's internalization and degradation effectively restricting iron release into the circulation and controlling systemic iron levels.
Biological function summary

This hormone plays a critical role in maintaining iron balance within the body by regulating both the absorption of dietary iron in the intestine and the release of stored iron from macrophages and hepatocytes. Hepcidin operates independently and is not part of any larger complex. Its levels fluctuate in response to various stimuli including iron levels inflammation and erythropoietic demand impacting iron transport and storage.

Pathways

Hepcidin is integral to the iron metabolism pathway. It interacts intricately with proteins such as transferrin and ferritin which are involved in iron transport and storage. Hepcidin's function is modulated by the BMP/SMAD signaling pathway as well where its expression is promoted by these signaling proteins. This regulation ensures that iron levels are properly maintained for critical processes such as hemoglobin synthesis and cellular respiration.

Hepcidin is connected to conditions such as anemia of chronic disease and hereditary hemochromatosis. In anemia of chronic disease increased hepcidin levels lead to iron sequestration and restricted availability which contributes to the anemic state. In hereditary hemochromatosis mutations can result in decreased hepcidin activity causing excessive iron accumulation in the body. Through these mechanisms hepcidin's interactions with proteins such as ferroportin and transferrin receptor 2 influence the development and progression of these iron-related disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma : absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.. Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
See full target information Hamp
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Product promise

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For full details, please see our Terms & Conditions

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