Skip to main content

Rabbit Recombinant Monoclonal HEPC2 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Recombinant fragment - Mouse, Mouse samples. Cited in 4 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB190775), expandable thumbnail
  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB190775), expandable thumbnail
  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB190775), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB190775), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (AB190775), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Mouse
Tested
Tested
Tested
Recombinant fragment - Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species
Recombinant fragment - Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Recombinant fragment - Mouse
Dilution info
-
Notes

-

Target data

Function

Seems to act as a signaling molecule involved in the maintenance of iron homeostasis.

Additional Targets

Hamp

Alternative names

Hepc2, Hamp2, Hepcidin-2

Recommended products

Rabbit Recombinant Monoclonal HEPC2 antibody. Suitable for WB, IHC-Fr, IHC-P and reacts with Recombinant fragment - Mouse, Mouse samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18937
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Hepcidin also known as LEAP-1 and Hepcidin-2 are small peptides with Hepcidin having a mass of approximately 2.8 kDa. These peptides synthesize in the liver and release into the circulatory system. Hepcidins regulate iron homeostasis by binding to the iron exporter ferroportin which is present on the surface of enterocytes macrophages and hepatocytes. This binding causes ferroportin internalization and degradation reducing iron release into the bloodstream.

Biological function summary

The iron-regulating proteins are key regulators of systemic iron metabolism. Hepcidin acts as a hormone controlling the distribution of iron a vital element for many physiological processes. Hepcidin-2 serves a similar role but its specific role is less defined compared to Hepcidin. Though they don't form complexes with other proteins their activity is critical for maintaining controlled levels of iron throughout the body preventing both deficiency and overload.

Pathways

Hepcidin and Hepcidin-2 are integral within the iron and hepcidin regulatory pathway which coordinates body iron levels. They play roles in the bone morphogenetic protein (BMP) signaling pathway. The pathway involves several other proteins like BMP6 and SMAD4 which influence hepcidin expression in response to iron levels and the body's erythropoietic activity.

Associated diseases and disorders

Hepcidin regulation has implications in conditions like hereditary hemochromatosis and anemia of inflammation. Hereditary hemochromatosis results from insufficient Hepcidin activity leading to iron overload. Conversely anemia of inflammation shows increased Hepcidin levels reducing iron availability and contributing to anemia. Hepcidin dysregulation connects to other proteins such as HFE and transferrin receptor 2 which play roles in iron overload disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Cytoplasmic staining on hepatocytes of Mouse liver was observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Highly expressed in the liver and to a much lesser extent in the heart (PMID:11113132 and PMID:12729891)

    All lanes: Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/200 dilution

    Lane 1: Mouse liver lysate at 10 µg

    Lane 2: Mouse brain lysate at 10 µg

    Lane 3: Mouse heart lysate at 10 µg

    Lane 4: Mouse kidney lysate at 10 µg

    Lane 5: Mouse spleen lysate at 10 µg

    Lane 6: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 7: NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 9 kDa

    Observed band size: 9 kDa

    Exposure time: 20s

  • Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time:

    Lane 1: 1 second.
    Lane 2: 2 seconds.

    The mouse recombinant fragment proteins contain aa24-83 with a GST-Tag and were made in-house.

    All lanes: Western blot - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775) at 1/5000 dilution

    Lane 1: Mouse Hepcidin recombinant fragment protein at 0.01 µg

    Lane 2: Mouse Hepcidin-2 recombinant fragment protein at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 9 kDa

    Observed band size: 32 kDa

  • Immunohistochemistry (Frozen sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized frozen section of Mouse liver labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077)secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on hepatocytes of Mouse liver.

    Counter stained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Mouse kidney. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Mouse spleen. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hepcidin + Hepcidin-2 antibody [EPR18937] (ab190775)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hepcidin + Hepcidin-2 with ab190775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Negative staining on Human colon cancer. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com