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Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus.

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Images

Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070), expandable thumbnail
  • Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070), expandable thumbnail
  • Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070), expandable thumbnail
  • Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04626

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Tested

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

The protein expressed by the ERBB2 gene is a protein tyrosine kinase involved in several cell surface receptor complexes, requiring a coreceptor for ligand binding. It is an essential component of a neuregulin-receptor complex, although neuregulins do not bind to it directly. GP30 is a potential ligand for this receptor. ERBB2 regulates the outgrowth and stabilization of peripheral microtubules (MTs) via the MEMO1-RHOA-DIAPH1 signaling pathway, which, upon activation, phosphorylates and inhibits GSK3B at the cell membrane. This prevents APC and CLASP2 phosphorylation, allowing their association with the cell membrane, facilitating MACF1 localization necessary for microtubule capture and stabilization. In the nucleus, ERBB2 is involved in transcriptional regulation, associating with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter to activate transcription. It is implicated in the transcriptional activation of CDKN1A, involving STAT3 and SRC, and participates in the transcription of rRNA genes by RNA Pol I, thereby enhancing protein synthesis and cell growth. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

Recommended products

Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P04626
Clone number
BLR241L
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

Associated diseases and disorders

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

Product promise

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7 product images

  • Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Detection of human HER2/ErbB2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

  • Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Detection of mouse HER2/ErbB2 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241CF) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

  • Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Samples: Whole cell lysate (10 µg) from LNCaP, HeLa, HT-29, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

    All lanes: Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070) at 1/1000 dilution

    Lane 1: LNCaP Whole cell lysate at 10 µg

    Lane 2: HeLa Whole cell lysate at 10 µg

    Lane 3: HT-29 Whole cell lysate at 10 µg

    Lane 4: HEK293T Whole cell lysate at 10 µg

    Lane 5: MCF-7 Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

    All lanes: Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070) at 1/1000 dilution

    Lane 1: NIH 3T3 Whole cell lysate at 10 µg

    Lane 2: CT26 Whole cell lysate at 10 µg

    Lane 3: TCMK-1 Whole cell lysate at 10 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L) (A700-241CF). Secondary: HRP-conjugated goat anti-rabbit IgG.

  • Immunocytochemistry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Immunocytochemistry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Sample: FFPE section of LNCaP cells. Antibody: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L) (A700-241CF). Secondary: HRP-conjugated goat anti-rabbit IgG .

  • Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070), expandable thumbnail

    Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241-1) used for IP at 20 µl/mg lysate. HER2/ErbB2 was also immunoprecipitated by a second antibody against a different epitope of HER2/ErbB2 (A301-621A). For blotting immunoprecipitated HER2/ErbB2, A700-241 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.

    All lanes: Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

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Product protocols

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