AB314070

Anti-HER2/ErbB2 antibody [BLR241L] - BSA free

Recombinant
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Rabbit Recombinant Monoclonal ErbB2 / HER2 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus.

View Alternative Names

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19

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Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Detection of human HER2/ErbB2 (shaded) in FaDu cells by flow cytometry. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Sample : FFPE section of human breast carcinoma. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L) (A700-241CF). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Sample : FFPE section of LNCaP cells. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L) (A700-241CF). Secondary : HRP-conjugated goat anti-rabbit IgG .

Supplier Data

Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241-1) used for IP at 20 µl/mg lysate. HER2/ErbB2 was also immunoprecipitated by a second antibody against a different epitope of HER2/ErbB2 (A301-621A). For blotting immunoprecipitated HER2/ErbB2, A700-241 was used at 1 : 1000. Detection : Chemiluminescence with an exposure time of 1 second.

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Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070)

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Flow Cyt

Supplier Data

Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Detection of mouse HER2/ErbB2 (shaded) in NIH3T3 cells by flow cytometry. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241CF) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Samples : Whole cell lysate (10 µg) from LNCaP, HeLa, HT-29, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070) at 1/1000 dilution

Lane 1:

LNCaP Whole cell lysate at 10 µg

Lane 2:

HeLa Whole cell lysate at 10 µg

Lane 3:

HT-29 Whole cell lysate at 10 µg

Lane 4:

HEK293T Whole cell lysate at 10 µg

Lane 5:

MCF-7 Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Samples : Whole cell lysate (10 µg) from NIH 3T3, CT26, and TCMK-1 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-HER2/ErbB2 recombinant monoclonal antibody [BLR241L] (A700-241) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : Chemiluminescence with an exposure time of 30 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (ab314070) at 1/1000 dilution

Lane 1:

NIH 3T3 Whole cell lysate at 10 µg

Lane 2:

CT26 Whole cell lysate at 10 µg

Lane 3:

TCMK-1 Whole cell lysate at 10 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR241L

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC, Flow Cyt, IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human ERBB2 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P04626

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.

Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.

See full target information ERBB2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HER2/ErbB2 antibody [BLR241L] - BSA free

Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Immunocytochemistry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Immunoprecipitation - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Flow Cytometry - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Western blot - Anti-HER2/ErbB2 antibody [BLR241L] - BSA free (AB314070)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise