Rabbit Polyclonal HERC1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC1 aa 250-400.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q15751
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Cow | Predicted |
| Horse | Predicted |
| Pig | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Cow, Pig | Dilution info - | Notes - |
Target data
Function
Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Alternative names
Probable E3 ubiquitin-protein ligase HERC1, HECT domain and RCC1-like domain-containing protein 1, HECT-type E3 ubiquitin transferase HERC1, p532, p619, HERC1
Recommended products
Rabbit Polyclonal HERC1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC1 aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC1 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q15751
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HERC1 also called HECT and RLD domain containing E3 ubiquitin protein ligase 1 possesses a significant molecular weight of approximately 532 kDa. The protein functions mechanically as an E3 ubiquitin ligase marking specific proteins for degradation by attaching ubiquitin molecules. HERC1 is expressed across various tissues including the brain indicating its widespread role in cellular regulation. The presence of HECT and RLD domains enables HERC1 to interact with other proteins and facilitate ubiquitination processes effectively.
- Biological function summary
HERC1 enhances cellular protein homeostasis by regulating protein turnover through ubiquitination. This protein participates in maintaining cellular equilibrium by tagging unwanted proteins for degradation preventing the accumulation of defective proteins. HERC1 often operates as part of multi-protein complexes ensuring precise control over cell signaling and various cellular activities. The strategic positioning of HERC1 in protein quality control mechanisms emphasizes its dynamic role in cellular health.
- Pathways
HERC1 plays a part in the ubiquitin-proteasome pathway and cell cycle regulation. The ubiquitin-proteasome pathway is essential for protein degradation and HERC1's function as an E3 ligase directly influences this system. Within this pathway HERC1 interacts with proteins such as ubiquitin-conjugating enzymes to facilitate orderly protein breakdown. Additionally HERC1 influences cell cycle regulation by controlling the stability of cycle-regulating proteins contributing to proper cell division and growth.
- Associated diseases and disorders
HERC1 is associated with neurodegenerative conditions and cancers. The role of HERC1 in protein ubiquitination and balance indicates its connection to neurological disorders like Alzheimer's disease where protein accumulation is a hallmark feature. Mutations in HERC1 have been linked to disruptions in ubiquitin pathways associating it with cancer development by impacting the degradation of key regulatory proteins. The interplay between HERC1 and the stability of oncogenic proteins accentuates its importance in understanding cancer pathophysiology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC1 antibody (ab151071)
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling HERC1 with ab151071 at 1/50 dilution.
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