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AB85832

Anti-HERC2 antibody

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(6 Publications)

Rabbit Polyclonal HERC2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human E3 ubiquitin-protein ligase HERC2 aa 4750 to C-terminus.

View Alternative Names

E3 ubiquitin-protein ligase HERC2, HECT domain and RCC1-like domain-containing protein 2, HECT-type E3 ubiquitin transferase HERC2, HERC2

2 Images
Western blot - Anti-HERC2 antibody (AB85832)
  • WB

Unknown

Western blot - Anti-HERC2 antibody (AB85832)

All lanes:

Western blot - Anti-HERC2 antibody (ab85832) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Predicted band size: 527 kDa

Observed band size: 160 kDa,260 kDa,50 kDa,527 kDa

true

Exposure time: 2min

Immunoprecipitation - Anti-HERC2 antibody (AB85832)
  • IP

Unknown

Immunoprecipitation - Anti-HERC2 antibody (AB85832)

Immunoprecipitation/ Western Blot
For IP :
Lane 1 : ab85832 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab85832 at 1μg/ml for WB
Whole cell lysate from HeLa cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-HERC2 antibody (ab85832)

Predicted band size: 527 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human E3 ubiquitin-protein ligase HERC2 aa 4750 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O95714

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HERC2 also known as E3 ubiquitin-protein ligase HERC2 acts mechanically as an enzyme involved in the ubiquitination process. It has a mass of approximately 527 kDa. HERC2 localizes in the nucleus and cytoplasm with a wide expression in many tissues including the brain and liver. It functions as an important regulator in protein homeostasis by tagging specific proteins for degradation via the proteasome.
Biological function summary

HERC2 plays a role in various cellular processes including DNA repair and cell cycle regulation. It forms complexes with other proteins such as USP7 and regulates p53 stability impacting processes like apoptosis and cell proliferation. Additionally HERC2 is important in maintaining genomic integrity by participating in DNA damage response pathways.

Pathways

Scientists observe HERC2 involvement in the DNA damage repair pathway and ubiquitin-proteasome system. HERC2 interacts with proteins such as BRCA1 which is important for homologous recombination repair and E3 ubiquitin ligases which coordinate protein degradation. Its function ensures proper regulation of cellular processes by controlling the turnover of key regulatory proteins.

Mutations or dysregulation of HERC2 associate with cancer and neurodevelopmental disorders. In cancer HERC2's interaction with p53 highlights its role in tumor suppression; aberrations in this pathway can lead to tumor development. In neurodevelopmental disorders its connection with the ATRX protein suggests implications in disorders such as autism spectrum disorders due to its role in chromatin remodeling and gene expression regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that regulates ubiquitin-dependent retention of repair proteins on damaged chromosomes. Recruited to sites of DNA damage in response to ionizing radiation (IR) and facilitates the assembly of UBE2N and RNF8 promoting DNA damage-induced formation of 'Lys-63'-linked ubiquitin chains. Acts as a mediator of binding specificity between UBE2N and RNF8. Involved in the maintenance of RNF168 levels. E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of XPA which influences the circadian oscillation of DNA excision repair activity. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed : 26692333). Modulates also iron metabolism by regulating the basal turnover of FBXL5 (PubMed : 24778179).
See full target information E3 ubiquitin-protein ligase HERC2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

iScience 24:102534 PubMed34142045

2021

ASPM promotes homologous recombination-mediated DNA repair by safeguarding BRCA1 stability.

Applications

Unspecified application

Species

Unspecified reactive species

Shibin Xu,Xingxuan Wu,Peipei Wang,Sheng-Li Cao,Bin Peng,Xingzhi Xu

Molecular medicine (Cambridge, Mass.) 27:39 PubMed33858325

2021

LncRNA-Fendrr protects against the ubiquitination and degradation of NLRC4 protein through HERC2 to regulate the pyroptosis of microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Qing Wang,Yue-Ying Zheng,Heng-Jun Zhou,Xiong-Xin Zhang,Pin Wu,Sheng-Mei Zhu

The Journal of biological chemistry 296:100246 PubMed33853758

2021

The deubiquitinase TRABID stabilizes the K29/K48-specific E3 ubiquitin ligase HECTD1.

Applications

Unspecified application

Species

Unspecified reactive species

Lee D Harris,Janic Le Pen,Nico Scholz,Juliusz Mieszczanek,Natalie Vaughan,Simon Davis,Georgina Berridge,Benedikt M Kessler,Mariann Bienz,Julien D F Licchesi

STAR protocols 1:100155 PubMed33377049

2020

Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuyang Liu,Zhaoyu Xue,Kendra R Vann,Xiaobing Shi,Tatiana G Kutateladze

Scientific reports 8:4092 PubMed29511261

2018

Insights into the ubiquitin-proteasome system of human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Saez,Seda Koyuncu,Ricardo Gutierrez-Garcia,Christoph Dieterich,David Vilchez

The Journal of biological chemistry 289:16430-41 PubMed24778179

2014

HERC2 targets the iron regulator FBXL5 for degradation and modulates iron metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiro Moroishi,Takayoshi Yamauchi,Masaaki Nishiyama,Keiichi I Nakayama
View all publications
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