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AB85732

Anti-HERC4 antibody

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(1 Publication)

Rabbit Polyclonal HERC4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human HERC4 aa 1000 to C-terminus.

View Alternative Names

KIAA1593, HERC4, Probable E3 ubiquitin-protein ligase HERC4, HECT domain and RCC1-like domain-containing protein 4, HECT-type E3 ubiquitin transferase HERC4

2 Images
Western blot - Anti-HERC4 antibody (AB85732)
  • WB

Unknown

Western blot - Anti-HERC4 antibody (AB85732)

All lanes:

Western blot - Anti-HERC4 antibody (ab85732) at 0.1 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 119 kDa

Observed band size: 119 kDa

true

Exposure time: 30s

Immunoprecipitation - Anti-HERC4 antibody (AB85732)
  • IP

Unknown

Immunoprecipitation - Anti-HERC4 antibody (AB85732)

Detection of Human HERC4 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85732 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-HERC4 antibody (ab85732)

Predicted band size: 119 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human HERC4 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q5GLZ8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-5.00000 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HERC4 also known as HECT and RLD domain-containing E3 ubiquitin protein ligase 4 is a protein that mechanically functions as an E3 ubiquitin ligase. Weighing approximately 120 kDa HERC4 facilitates the transfer of ubiquitin from E2 ubiquitin-conjugating enzymes to substrate proteins tagging them for degradation by the proteasome. The protein is expressed in various tissues including the brain heart and lung indicating its broad functional significance across different biological systems.
Biological function summary

HERC4 plays an important role in regulating protein turnover and cellular processes. As part of the ubiquitin-proteasome system it ensures protein quality control and homeostasis by targeting misfolded or damaged proteins. While HERC4 does not traditionally form complexes its activity influences the stability and function of other proteins within the cell. This implicates HERC4 in not only cellular maintenance but also processes such as cell cycle regulation and signal transduction.

Pathways

The ubiquitin-proteasome pathway involves HERC4 contributing to the degradation of regulatory proteins that control various cellular activities. The protein interacts with pathways like cell cycle progression and stress response. It maintains a functional relationship with proteins such as p53 a known tumor suppressor and other E3 ligases that manage the proteolytic balance in the cell demonstrating its integration into critical regulatory networks.

HERC4 has connections to cancer and neurodegenerative diseases. In cancer aberrant ubiquitination activities involving HERC4 can disrupt normal protein regulation leading to uncontrolled cell growth. It may interact with oncogenic proteins that escape degradation promoting tumor progression. In neurodegenerative disorders such as Parkinson's disease dysregulation of the ubiquitin-proteasome system potentially involving HERC4 connects with proteins like Parkin impacting neuronal survival and function. Understanding HERC4's role in these pathways provides insights into potential therapeutic targets for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.
See full target information HERC4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Protein & cell 10:595-605 PubMed30710319

2019

A miRNA-HERC4 pathway promotes breast tumorigenesis by inactivating tumor suppressor LATS1.

Applications

Unspecified application

Species

Unspecified reactive species

Youqin Xu,Kaiyuan Ji,Meng Wu,Bingtao Hao,Kai-Tai Yao,Yang Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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AB221757

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herc4-antibody-ab221757

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