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AB99949

Anti-HERC6 antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal HERC6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC6 aa 100 to C-terminus.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (AB99949)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HERC6 antibody (AB99949)

ab99949 at 1/100 dilution detecting HERC6 expression in formalin-fixed, paraffin-embedded Human fetal testis tissue.
Note cytoplasmic staining.

Western blot - Anti-HERC6 antibody (AB99949)
  • WB

Unknown

Western blot - Anti-HERC6 antibody (AB99949)

All lanes:

Western blot - Anti-HERC6 antibody (ab99949) at 1/1000 dilution

All lanes:

HeLa cell lysate

Predicted band size: 115 kDa

Observed band size: 115 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Probable E3 ubiquitin-protein ligase HERC6 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IVU3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.02% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HERC6 also known as HECT and RLD domain containing E3 ubiquitin protein ligase 6 plays a mechanical role in the ubiquitination process. It is a part of the HERC family of ubiquitin ligases characterized by an E3 ubiquitin-ligase domain. HERC6 has an approximate molecular mass of 119 kDa. This protein is ubiquitously expressed across various tissues with especially high levels found in the spleen lung and testis. Its mechanical role involves tagging proteins for degradation which is an important function in regulating protein levels.
Biological function summary

HERC6 contributes to the cellular antiviral response. It participates in the conjugation of the ubiquitin-like protein ISG15 (interferon-stimulated gene 15) to target proteins a modification known as ISGylation. This function supports its participation in host defense mechanisms against viral infections. As an enzyme HERC6 controls ISGylation events either independently or as part of larger protein complexes influencing various cellular processes.

Pathways

The cellular role of HERC6 intersects with the ISGylation pathway. This process involves several enzymes including the E1-activating ISG15 and E2-conjugating enzymes. Additionally HERC6 has connections to the proteasome degradation pathway where ubiquitination marks proteins for breakdown. This protein works in concert with other components like HERC5 another E3 ligase to facilitate antiviral defense through the modification of viral and host proteins.

HERC6 is associated with conditions involving viral infection and immune response. Its dysfunction can potentially impact the efficiency of the immune defense mechanism making the host more susceptible to viruses like influenza or HIV. As it works closely with ISG15 altered expression or activity of HERC6 or ISG15 could play a role in such viral-related diseases. It may also influence cancer development as disruptions in protein degradation pathways are commonly linked with tumor progression highlighting the need for further research into its pathophysiological roles.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
See full target information Probable E3 ubiquitin-protein ligase HERC6

Alternative Names

Probable E3 ubiquitin-protein ligase HERC6, HECT domain and RCC1-like domain-containing protein 6, HECT-type E3 ubiquitin transferase HERC6, HERC6
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9550 PubMed39500869

2024

Inhibition of GPX4 enhances CDK4/6 inhibitor and endocrine therapy activity in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

M T Herrera-Abreu,J Guan,U Khalid,J Ning,M R Costa,J Chan,Q Li,J-P Fortin,W R Wong,P Perampalam,A Biton,W Sandoval,J Vijay,M Hafner,R Cutts,G Wilson,J Frankum,T I Roumeliotis,J Alexander,O Hickman,R Brough,S Haider,J Choudhary,C J Lord,A Swain,C Metcalfe,N C Turner
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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