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AB248983

Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HERPUD1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HERP, KIAA0025, MIF1, HERPUD1, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, Methyl methanesulfonate (MMF)-inducible fragment protein 1

4 Images
Flow Cytometry (Intracellular) - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)

This data was developed using ab150424, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HepG2 cells labelling HERPUD1 with ab150424 at 1/10 dilution (red) or a rabbit IgG (negative).

Immunocytochemistry/ Immunofluorescence - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)

This data was developed using ab150424, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC-3 cells labelling HERPUD1 with ab150424 at 1/100 dilution.

Western blot - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)
  • WB

Lab

Western blot - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)

This data was developed using ab150424, the same antibody clone in a different buffer formulation.

Western blot : Anti-HERPUD1 antibody [EPR9649] ab150424 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 49 kDa in Wild-type A549 cell lysates with no signal observed at this size in HERPUD1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HERPUD1 antibody [EPR9649] (<a href='/en-us/products/primary-antibodies/herpud1-antibody-epr9649-ab150424'>ab150424</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

HERPUD1 knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 49 kDa,76 kDa

false

Western blot - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)
  • WB

Unknown

Western blot - Anti-HERPUD1 antibody [EPR9649] - BSA and Azide free (AB248983)

This data was developed using ab150424, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HERPUD1 antibody [EPR9649] (<a href='/en-us/products/primary-antibodies/herpud1-antibody-epr9649-ab150424'>ab150424</a>) at 1/1000 dilution

Lane 1:

BxPC-3 cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9649

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248983 is the carrier-free version of ab150424.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HERPUD1 also known as Herp is a protein involved in the cellular response to stress especially related to the endoplasmic reticulum (ER). It has a molecular mass of approximately 42 kDa. This protein is expressed in various tissues with high expression levels found in the liver heart and muscle. HERPUD1 becomes particularly upregulated during conditions that cause ER stress helping cells manage misfolded proteins.
Biological function summary

HERPUD1 helps maintain cellular homeostasis during periods of ER stress by facilitating the degradation of misfolded proteins. It forms part of a larger complex in the ER-associated degradation (ERAD) pathway which targets these proteins for retrotranslocation and subsequent destruction by the proteasome. HERPUD1 therefore plays a vital role in mitigating the buildup of potentially harmful proteins within the cell.

Pathways

HERPUD1 is integral to the unfolded protein response (UPR) and the ERAD pathway. In the UPR HERPUD1 is involved in signaling mechanisms that help restore normal ER function. It interacts with other proteins such as SEL1L and DERLIN-1 within the ERAD pathway to ensure the proper disposal of misfolded proteins preventing cellular damage.

HERPUD1 is connected with neurodegenerative diseases and cardiac disorders. Its dysfunction or dysregulation can lead to the accumulation of misfolded proteins contributing to conditions like Alzheimer's disease. Moreover HERPUD1's relationship with proteins such as ubiquitin suggests its involvement in cardiac stress responses which can be significant in heart failure. HERPUD1's functions and interactions underline its importance in cellular stress responses and associated disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed : 16289116, PubMed : 28827405). Could enhance presenilin-mediated amyloid-beta protein 40 generation. Binds to ubiquilins and this interaction is required for efficient degradation of CD3D via the ERAD pathway (PubMed : 18307982).
See full target information HERPUD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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