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AB319031

Anti-HERV-K-env antibody [EPR27172-7]

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Rabbit Recombinant Monoclonal HERV-K-env antibody. Suitable for WB and reacts with Transfected cell lysate - Human, Human samples.
3 Images
Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)
  • WB

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)

The expression of HERV-K-env is upregulated in response to estradiol treatment (PMID : 12629516).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (ab319031) at 1/1000 dilution

Lane 1:

Untreated MCF7 (human breast adenocarcinoma epithelial cell) starved for 24 hours, whole cell lysate at 20 µg

Lane 2:

MCF7 starved for 24 hours, then treated with 10nM 17beta-Estradiol for 72 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 26s

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)
  • WB

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)

Low expression : 22Rv1(PMID : 22247020), HEK-293.

The identity of the band beneath 75 kDa is unknown.

The expression profile is consistent with what has been described in the literature (PMID : 22247020).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (ab319031) at 1/1000 dilution

Lane 1:

DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 114s

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)
  • WB

Supplier Data

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (AB319031)

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-HERV-K-env antibody [EPR27172-7] (ab319031) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human HERV-K-env expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,124 kDa

false

Exposure time: 15s

  • Carrier free

    Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27172-7

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HERV-K-env also known as Human Endogenous Retrovirus K envelope protein has a mass of approximately 72 kDa. Mechanically HERV-K-env functions as a fusogenic protein facilitating the fusion of viral membranes with host cellular membranes. It is expressed in a variety of tissues with notable expression in germ cell tumor tissues and placental tissues. As a member of the envelope proteins in the HERV family it comprises part of the retroviral sequences integrated into the human genome.
Biological function summary

The envelope protein of the HERV-K family plays a role in cell-cell fusion and possibly in immune evasion. It is not part of a larger complex but interacts with cell surface receptors to mediate entry into cells. This function allows it to participate in the remodeling of cell membranes which relates to processes involved in early embryonic development and differentiation. Understanding its activity helps expand the knowledge on cellular transformation and tumorigenesis.

Pathways

HERV-K-env is involved in fusion and integration pathways. It engages in pathways associated with viral entry mechanisms akin to contemporary retroviruses. Notably it interrelates with pathways that include the interaction of viral proteins with host cell receptors sharing some mechanistic similarities with proteins such as Env from HIV. Through these pathways HERV-K-env influences the cellular environment in a manner that could redirect cellular signaling.

There is a connection between HERV-K-env expression and certain cancers including germ cell tumors and melanoma. Expression of HERV-K-env in these malignancies may contribute to oncogenic processes and immune evasion. Additionally it has links to autoimmune disorders potentially affecting proteins such as Toll-like receptors which are involved in immune system activation. Understanding its role in these diseases aids in developing potential therapeutic strategies targeting endogenous retroviral elements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HERV-K-env
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com