Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free
- Recombinant
- RabMAb
- What is this?
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![Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)](https://content.abcam.com/products/images/herv-k-env-antibody-epr27172-7-bsa-and-azide-free-ab319032--western-blot-img433446.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)
This data was developed using ab319031, the same antibody clone in a different buffer formulation.
The expression of HERV-K-env is upregulated in response to estradiol treatment (PMID : 12629516).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/en-us/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution
Lane 1:
Untreated MCF7 (human breast adenocarcinoma epithelial cell) starved for 24 hours, whole cell lysate at 20 µg
Lane 2:
MCF7 starved for 24 hours, then treated with 10nM 17beta-Estradiol for 72 hours, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75 kDa,36 kDa
false
Exposure time: 26s
![Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)](https://content.abcam.com/products/images/herv-k-env-antibody-epr27172-7-bsa-and-azide-free-ab319032--western-blot-img433445.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)
This data was developed using ab319031, the same antibody clone in a different buffer formulation.
Low expression : 22Rv1(PMID : 22247020), HEK-293.
The identity of the band beneath 75 kDa is unknown.
The expression profile is consistent with what has been described in the literature (PMID : 22247020).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/en-us/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution
Lane 1:
DU 145 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3:
22Rv1 (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75 kDa,36 kDa
false
Exposure time: 114s
![Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)](https://content.abcam.com/products/images/herv-k-env-antibody-epr27172-7-bsa-and-azide-free-ab319032--western-blot-img433447.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Supplier Data
Western blot - Anti-HERV-K-env antibody [EPR27172-7] - BSA and Azide free (AB319032)
This data was developed using ab319031, the same antibody clone in a different buffer formulation.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
All lanes:
Western blot - Anti-HERV-K-env antibody [EPR27172-7] (<a href='/en-us/products/primary-antibodies/herv-k-env-antibody-epr27172-7-ab319031'>ab319031</a>) at 1/1000 dilution
Lane 1:
293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg
Lane 2:
293T cells transfected with a human HERV-K-env expression vector containing a myc-His-tag®, whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 75 kDa,124 kDa
false
Exposure time: 15s
Related conjugates and formulations (1)
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Anti-HERV-K-env antibody [EPR27172-7]
Reactivity data
Product details
ab319032 is the carrier-free version of ab319031.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The envelope protein of the HERV-K family plays a role in cell-cell fusion and possibly in immune evasion. It is not part of a larger complex but interacts with cell surface receptors to mediate entry into cells. This function allows it to participate in the remodeling of cell membranes which relates to processes involved in early embryonic development and differentiation. Understanding its activity helps expand the knowledge on cellular transformation and tumorigenesis.
Pathways
HERV-K-env is involved in fusion and integration pathways. It engages in pathways associated with viral entry mechanisms akin to contemporary retroviruses. Notably it interrelates with pathways that include the interaction of viral proteins with host cell receptors sharing some mechanistic similarities with proteins such as Env from HIV. Through these pathways HERV-K-env influences the cellular environment in a manner that could redirect cellular signaling.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com