Anti-Hes1 antibody

• WB

AbReview50107****

Western blot - Anti-Hes1 antibody (AB71559)

Blocked with 5% milk at RT

All lanes:

Western blot - Anti-Hes1 antibody (ab71559) at 1/2000 dilution

All lanes:

MCF-7 at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/20000 dilution

Predicted band size: 30 kDa

true

Exposure time: 15s

This image is courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Anti-Hes1 antibody (AB71559)

Actin blot given as control.

All lanes:

Western blot - Anti-Hes1 antibody (ab71559)

Lane 1:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)

Lane 2:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)

Lane 3:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)

Lane 4:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)

Lane 5:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)

Lane 6:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)

Lane 7:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

Lane 8:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

Predicted band size: 30 kDa

false

• ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody (AB71559)

Immunocytochemistry-immunofluorescence using Anti-Hes1 antibody, ab71559. Publication image from Rubinsztein, D. C. et al., 2016, Nat Commun, 26837467. Legend direct from paper.

Atg16L1 hypomorphs show developmental retention in the gut.(a) Effect of decreased Atg16L1 levels on growth of gut villi length. Representative gut section from wild-type and Atg16L1 hypomorph (Atg16hyp) 10–15 weeks old mice. (b) Quantification of villi length from wild-type and Atg16L1 hypomorph (Atg16hyp) 10–15 weeks old mice. **P<0.01 by unpaired t-test. n=4. Error bars=s.e.m. (c) Hes1 immunostaining in gut from wild-type and Atg16L1 hypomorph (Atg16hyp) mice. (d) Grem1 immunostaining in gut from wild-type and Atg16L1 hypomorph (Atg16hyp) mice. (e) Quantification of Grem1-positive cells per crypt in wild-type and Atg16L1 hypomorph (Atg16hyp) mice. **P<0.01 by paired t-test. n=3. Error bars=s.e.m. Scale bar, 50 µm.