AB71559

Anti-Hes1 antibody

Name: Anti-Hes1 antibody (ab71559) | Abcam
Brand: Abcam Limited
SKU: AB71559
Price: 525 USD
Availability: InStock
Rating: 4 (26 reviews)

(26 Reviews)

(158 Publications)

Anti-Hes1 antibody (ab71559) is a rabbit polyclonal antibody detecting Hes1 in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse.

- Over 130 publications
- Trusted since 2008

View Alternative Names

BHLHB39, HL, HRY, HES1, Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, bHLHb39, hHL

2 Images

AbReview50107****

Western blot - Anti-Hes1 antibody (AB71559)

Blocked with 5% milk at RT

All lanes:

Western blot - Anti-Hes1 antibody (ab71559) at 1/2000 dilution

All lanes:

MCF-7 at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/20000 dilution

Predicted band size: 30 kDa

true

Exposure time: 15s

This image is courtesy of an anonymous Abreview

Unknown

Western blot - Anti-Hes1 antibody (AB71559)

Actin blot given as control.

All lanes:

Western blot - Anti-Hes1 antibody (ab71559)

Lane 1:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)

Lane 2:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 1)

Lane 3:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)

Lane 4:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 2)

Lane 5:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)

Lane 6:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 3)

Lane 7:

Normal kidney tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

Lane 8:

Tumor tissue taken from a patient of sporadic angiomyolipoma. (Patient 4)

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-Fr, ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human HES1. The exact immunogen used to generate this antibody is proprietary information.

Q14469

Specificity

This antibody reacts specifically with mouse 30 kDa Hes1 protein.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-Hes1 antibody (ab71559) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Hes1?
Anti-Hes1 (ab71559) specifically detects a band for Hes1 (UniProt: Q14469) at a molecular weight of 30kDa.

Trusted by the scientific community
Anti-Hes1 (ab71559) was first used in a scientific publication in 2008 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists
Anti-Hes1 (ab71559) has over 25 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs : 5'-CACNAG-3' with high affinity and on E-box motifs : 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

See full target information HES1

style

left-column

Publications (158)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reports 20:102612 PubMed40882640

2025

Pen-2 regulates glial homeostasis by coordinating self-renewal and transdifferentiation programs in oligodendrocyte precursor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huiru Bi,Jinxing Hou,Wenkai Shao,Chenyi Ge,Yang Liu,Runmin Wang,Guiquan Chen,Yun Xu,Zhiye Wang

Cell biology and toxicology 41:46 PubMed39976779

2025

Impaired olfactory bulb neurogenesis mediated by Notch1 contributes to olfactory dysfunction in mice chronically exposed to methamphetamine.

Applications

Unspecified application

Species

Unspecified reactive species

Cihang Gu,Zhuo Wang,Wenyu Luo,Haosen Ling,Xilie Cui,Tongtong Deng,Kuan Li,Wei Huang,Qiqian Xie,Bowen Tao,Xiaolan Qi,Xiaojia Peng,Jiuyang Ding,Pingming Qiu

Journal of tissue engineering 16:20417314251315185 PubMed39882545

2025

Therapeutic potential and mechanisms of umbilical cord mesenchymal stem cells differentiating into tendon cells and promotion of rotator cuff tendon-bone healing.

Applications

Unspecified application

Species

Unspecified reactive species

Youliang Shen,Yuelei Wang,Yidan Xu,Jie Wang,Chuqiang Yin,Zengshuai Han,Feng Shen,Ting Wang

Journal of biochemical and molecular toxicology 39:e70129 PubMed39878097

2025

Estrogen Promotes the Proliferation and Migration of Endometrial Cancer Through the GPER-Mediated NOTCH Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Qi,Yuxi Jin,Lulu Si,Hanlin Fu,Xiaojing Shi,Yana Liu,Yifan Wang,Ruixia Guo

American journal of physiology. Cell physiology 327:C929-C945 PubMed39099421

2024

Modulating endothelial cell dynamics in fat grafting: the impact of DLL4 siRNA via adipose stem cell extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Sen-Lin Deng,Qiang Fu,Qing Liu,Fu-Jun Huang,Miao Zhang,Xun Zhou

BMC complementary medicine and therapies 24:293 PubMed39090706

2024

The effect of salidroside in promoting endogenous neural regeneration after cerebral ischemia/reperfusion involves notch signaling pathway and neurotrophic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Jiabing Zheng,Jizhou Zhang,Jing Han,Zhichang Zhao,Kan Lin

Experimental and therapeutic medicine 28:373 PubMed39091630

2024

Interference of FZD2 suppresses proliferation, vasculogenic mimicry and stemness in glioma cells via blocking the Notch/NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuge Ran,Shuai Han,Dongxue Gao,Xiaobo Chen,Chan Liu

International journal of biological sciences 20:3317-3333 PubMed38993555

2024

Ursolic Acid Alleviates Mitotic Catastrophe in Podocyte by Inhibiting Autophagic P62 Accumulation in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hang Mei,Tienan Jing,Haojun Liu,Yue Liu,Xinwang Zhu,Jiao Wang,Li Xu

Cell reports 43:113680 PubMed38241148

2024

Notch receptor-ligand binding facilitates extracellular vesicle-mediated neuron-to-neuron communication.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Zhi Wang,Charlotte C M Castillon,Kamil K Gebis,Elizabeth T Bartom,Alessandra d'Azzo,Anis Contractor,Jeffrey N Savas

Allergologia et immunopathologia 52:71-78 PubMed38186196

2024

Studies on the effect and mechanism of CD147 on melanoma stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Jiang,Renyi Liang,Liqun Li,Jian Guan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hes1 antibody

Western blot - Anti-Hes1 antibody (AB71559)

Western blot - Anti-Hes1 antibody (AB71559)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (158)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise