AB119776

Anti-Hes1 antibody [OTI2D2]

Name: Anti-Hes1 antibody [OTI2D2] (ab119776) | Abcam
Brand: Abcam Limited
SKU: AB119776
Price: 520 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(19 Publications)

Mouse Monoclonal HES1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate, Human, Transfected cell line samples. Cited in 19 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HES1.

View Alternative Names

BHLHB39, HL, HRY, HES1, Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, bHLHb39, hHL

3 Images

Flow Cytometry (Intracellular) - Anti-Hes1 antibody [OTI2D2] (AB119776)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Hes1 antibody [OTI2D2] (AB119776)

ab119776, at 1/100 dilution, staining Hes1 (red) in Jurkat cells, compared to a nonspecific negative control antibody,(blue) by Flow cytometry (Intracellular).

This image was generated using the ascites version of the product.

Unknown

Western blot - Anti-Hes1 antibody [OTI2D2] (AB119776)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC211709)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Hes1 antibody [OTI2D2] (ab119776) at 1/2000 dilution

Lane 1:

HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK293T cell lysate transfected with pCMV6-ENTRY Hes1 cDNA at 5 µg

Predicted band size: 30 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [OTI2D2] (AB119776)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [OTI2D2] (AB119776)

ab119776, at 1/100 dilution, staining Hes1 in COS7 cells transiently transfected with pCMV6-ENTRY Hes1 cDNA by Immunofluorescence.

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2D2

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HES1.

Q14469

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Dilute in PBS (pH7.3) before use.

This product was changed from ascites to tissue culture supernatant on the 4^th of June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs : 5'-CACNAG-3' with high affinity and on E-box motifs : 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

See full target information HES1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 14:35 PubMed38494478

2024

Deubiquitinase PSMD7 facilitates pancreatic cancer progression through activating Nocth1 pathway via modifying SOX2 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Luo,Yi Yu,Jinfeng Zhu,Leifeng Chen,Dan Li,Xingyu Peng,Zitao Liu,Qing Li,Qing Cao,Kai Huang,Rongfa Yuan

Arteriosclerosis, thrombosis, and vascular biology 44:653-665 PubMed38269590

2024

Single-Cell RNA-Seq Reveals Coronary Heterogeneity and Identifies CD133TRPV4 Endothelial Subpopulation in Regulating Flow-Induced Vascular Tone in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aiqin Mao,Ka Zhang,Hao Kan,Mengru Gao,Zhiwei Wang,Tingting Zhou,Jing Shao,Dongxu He

Open medicine (Warsaw, Poland) 18:20230634 PubMed37082613

2023

F-box and WD repeat domain containing 7 inhibits the activation of hepatic stellate cells by degrading delta-like ligand 1 to block Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Sun,Lili He,Peiran Guo,Fenghua Li,Bo Wang,Yifan Zhang,Kai An,Ming Peng

Nature communications 13:6117 PubMed36253375

2022

Brain milieu induces early microglial maturation through the BAX-Notch axis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangying Zhao,Jiangyong He,Jun Tang,Nianfei Cui,Yanyan Shi,Zhifan Li,Shengnan Liu,Yazhou Wang,Ming Ma,Congjian Zhao,Lingfei Luo,Li Li

Oxidative medicine and cellular longevity 2022:7676872 PubMed36238644

2022

Lead Exposure Induced Neural Stem Cells Death via Notch Signaling Pathway and Gut-Brain Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Sun,Yuankang Zou,Peng Su,Chong Xue,Diya Wang,Fang Zhao,Wenjing Luo,Jianbin Zhang

Cell death & disease 13:306 PubMed35383153

2022

Nogo-B promotes angiogenesis and improves cardiac repair after myocardial infarction via activating Notch1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjun Zheng,Jingrong Lin,Dingsheng Liu,Guoqing Wan,Xuefeng Gu,Jian Ma

Journal of cell science 135: PubMed34982151

2022

High-content image analysis to study phenotypic heterogeneity in endothelial cell monolayers.

Applications

Unspecified application

Species

Unspecified reactive species

Francois Chesnais,Jonas Hue,Errin Roy,Marco Branco,Ruby Stokes,Aize Pellon,Juliette Le Caillec,Eyad Elbahtety,Matteo Battilocchi,Davide Danovi,Lorenzo Veschini

Journal of materials science. Materials in medicine 33:4 PubMed34940936

2021

Hydrogel contained valproic acid accelerates bone-defect repair via activating Notch signaling pathway in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Tian-Lin Li,Hong-Guang Xu,Min Yang

Circulation. Genomic and precision medicine 14:e003142 PubMed33517678

2021

Whole-Transcriptome Profiling of Human Heart Tissues Reveals the Potential Novel Players and Regulatory Networks in Different Cardiomyopathy Subtypes of Heart Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Feng Liu,Ying Ni,Christine S Moravec,Michael Morley,Euan A Ashley,Thomas P Cappola,Kenneth B Margulies,W H Wilson Tang

Cells 9: PubMed32993109

2020

Integrated Transcriptome and Proteome Analyses Reveal the Regulatory Role of miR-146a in Human Limbal Epithelium via Notch Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Adam J Poe,Mangesh Kulkarni,Aleksandra Leszczynska,Jie Tang,Ruchi Shah,Yasaman Jami-Alahmadi,Jason Wang,Andrei A Kramerov,James Wohlschlegel,Vasu Punj,Alexander V Ljubimov,Mehrnoosh Saghizadeh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hes1 antibody [OTI2D2]

Flow Cytometry (Intracellular) - Anti-Hes1 antibody [OTI2D2] (AB119776)

Western blot - Anti-Hes1 antibody [OTI2D2] (AB119776)

Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody [OTI2D2] (AB119776)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (19)

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