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AB246949

Anti-HESX1 antibody

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(1 Publication)

Rabbit Polyclonal HESX1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HESX1 aa 1-150.

View Alternative Names

HANF, HESX1, Homeobox expressed in ES cells 1, Homeobox protein ANF, hAnf

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)

Paraffin-embedded human skeletal muscle tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis. No nuclear positivity in striated muscle fibers as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)

Paraffin-embedded human skin tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (AB246949)

Paraffin-embedded human testis tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HESX1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9UBX0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HESX1 also known as Homeobox expressed in ES cells 1 is a homeobox gene encoding a protein with a molecular mass of approximately 22 kDa. This protein acts as a transcriptional repressor and is expressed in the early stages of embryonic development particularly within the anterior pituitary gland and developing forebrain. The HESX1 protein interacts with DNA sequences to regulate the expression of target genes essential for forebrain and pituitary formation.
Biological function summary

HESX1 functions as a transcriptional repressor influencing the development of head and pituitary embryogenesis. This protein is not part of a larger complex but works by binding directly to promoter regions of DNA affecting transcriptional activity. HESX1 plays a critical role in regulating the expression of genes necessary for early development and cellular differentiation ensuring proper formation of the forebrain and pituitary gland.

Pathways

HESX1 is involved in important developmental pathways including the Wnt signaling and Sonic Hedgehog pathways. These pathways govern cellular processes such as proliferation differentiation and morphogenesis. HESX1's role in these pathways connects it with proteins like β-catenin in the Wnt signaling and SHH protein in the Sonic Hedgehog pathway which are essential for maintaining the balance of cell growth and organ development.

Mutations in HESX1 are associated with septo-optic dysplasia and combined pituitary hormone deficiency. These conditions affect brain and pituitary development leading to clinical manifestations like optic nerve hypoplasia and hormone deficiencies. The connection of HESX1 with proteins like LHX3 in these disorders highlights its influential role in developmental regulation and disease manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.
See full target information HESX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 16:1006-1018 PubMed38577450

2024

Ubiquitin-specific protease 21 promotes tumorigenicity and stemness of colorectal cancer by deubiquitinating and stabilizing ZEB1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jun Lin,Ye-Cai Lu
View all publications
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Product promise

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