Rabbit Polyclonal HESX1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HESX1 aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HESX1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBX0
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target data
Function
Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.
Alternative names
HANF, HESX1, Homeobox expressed in ES cells 1, Homeobox protein ANF, hAnf
Recommended products
Rabbit Polyclonal HESX1 antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HESX1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HESX1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UBX0
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HESX1 also known as Homeobox expressed in ES cells 1 is a homeobox gene encoding a protein with a molecular mass of approximately 22 kDa. This protein acts as a transcriptional repressor and is expressed in the early stages of embryonic development particularly within the anterior pituitary gland and developing forebrain. The HESX1 protein interacts with DNA sequences to regulate the expression of target genes essential for forebrain and pituitary formation.
- Biological function summary
HESX1 functions as a transcriptional repressor influencing the development of head and pituitary embryogenesis. This protein is not part of a larger complex but works by binding directly to promoter regions of DNA affecting transcriptional activity. HESX1 plays a critical role in regulating the expression of genes necessary for early development and cellular differentiation ensuring proper formation of the forebrain and pituitary gland.
- Pathways
HESX1 is involved in important developmental pathways including the Wnt signaling and Sonic Hedgehog pathways. These pathways govern cellular processes such as proliferation differentiation and morphogenesis. HESX1's role in these pathways connects it with proteins like β-catenin in the Wnt signaling and SHH protein in the Sonic Hedgehog pathway which are essential for maintaining the balance of cell growth and organ development.
- Associated diseases and disorders
Mutations in HESX1 are associated with septo-optic dysplasia and combined pituitary hormone deficiency. These conditions affect brain and pituitary development leading to clinical manifestations like optic nerve hypoplasia and hormone deficiencies. The connection of HESX1 with proteins like LHX3 in these disorders highlights its influential role in developmental regulation and disease manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (ab246949)
Paraffin-embedded human testis tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (ab246949)
Paraffin-embedded human skin tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HESX1 antibody (ab246949)
Paraffin-embedded human skeletal muscle tissue stained for HESX1 using ab246949 at 1/50 dilution in immunohistochemical analysis. No nuclear positivity in striated muscle fibers as expected.
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