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AB67728

Anti-HESX1 antibody

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(1 Publication)

Mouse Polyclonal HESX1 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HESX1.

View Alternative Names

HANF, HESX1, Homeobox expressed in ES cells 1, Homeobox protein ANF, hAnf

1 Images
Western blot - Anti-HESX1 antibody (AB67728)
  • WB

Unknown

Western blot - Anti-HESX1 antibody (AB67728)

All lanes:

Western blot - Anti-HESX1 antibody (ab67728) at 1/500 dilution

Lane 1:

HESX1 transfected lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Secondary

All lanes:

Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

Predicted band size: 21 kDa

Observed band size: 24 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HESX1.

Q9UBX0

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HESX1 also known as Homeobox expressed in ES cells 1 is a homeobox gene encoding a protein with a molecular mass of approximately 22 kDa. This protein acts as a transcriptional repressor and is expressed in the early stages of embryonic development particularly within the anterior pituitary gland and developing forebrain. The HESX1 protein interacts with DNA sequences to regulate the expression of target genes essential for forebrain and pituitary formation.
Biological function summary

HESX1 functions as a transcriptional repressor influencing the development of head and pituitary embryogenesis. This protein is not part of a larger complex but works by binding directly to promoter regions of DNA affecting transcriptional activity. HESX1 plays a critical role in regulating the expression of genes necessary for early development and cellular differentiation ensuring proper formation of the forebrain and pituitary gland.

Pathways

HESX1 is involved in important developmental pathways including the Wnt signaling and Sonic Hedgehog pathways. These pathways govern cellular processes such as proliferation differentiation and morphogenesis. HESX1's role in these pathways connects it with proteins like β-catenin in the Wnt signaling and SHH protein in the Sonic Hedgehog pathway which are essential for maintaining the balance of cell growth and organ development.

Mutations in HESX1 are associated with septo-optic dysplasia and combined pituitary hormone deficiency. These conditions affect brain and pituitary development leading to clinical manifestations like optic nerve hypoplasia and hormone deficiencies. The connection of HESX1 with proteins like LHX3 in these disorders highlights its influential role in developmental regulation and disease manifestation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.
See full target information HESX1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Endocrine journal 63:405-10 PubMed26781211

2016

A novel mutation in HESX1 causes combined pituitary hormone deficiency without septo optic dysplasia phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Masaki Takagi,Mai Takahashi,Yoshiaki Ohtsu,Takeshi Sato,Satoshi Narumi,Hirokazu Arakawa,Tomonobu Hasegawa
View all publications
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