AB230488

Anti-HEXA antibody

Name: Anti-HEXA antibody (ab230488) | Abcam
Brand: Abcam Limited
SKU: AB230488

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(2 Publications)

Rabbit Polyclonal HEXA antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human HEXA aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, N-acetyl-beta-glucosaminidase subunit alpha, Hexosaminidase subunit A, HEXA

4 Images

Flow Cytometry (Intracellular) - Anti-HEXA antibody (AB230488)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HEXA antibody (AB230488)

Intracellular flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling HEXA with ab230488 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Supplier Data

Western blot - Anti-HEXA antibody (AB230488)

All lanes:

Western blot - Anti-HEXA antibody (ab230488) at 1/1000 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Predicted band size: 61 kDa

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Supplier Data

Western blot - Anti-HEXA antibody (AB230488)

All lanes:

Western blot - Anti-HEXA antibody (ab230488) at 1/1000 dilution

All lanes:

Mouse liver lysate at 35 µg

Predicted band size: 61 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody (AB230488)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody (AB230488)

Formalin-fixed, paraffin-embedded human brain cortex tissue stained for HEXA using ab230488 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human HEXA aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P06865

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HEXA gene codes for the enzyme Hexosaminidase A also known as hexosaminidase alpha or HEXA subunit. This enzyme has several subunits with a molecular mass of approximately 58 kDa. HEXA expresses in various tissues but it shows high expression in the brain and other neural tissues. Mechanically Hexosaminidase A is involved in the hydrolysis of GM2 gangliosides into GM3 by removing the N-acetylgalactosamine residue which plays a role in the degradation of glycolipids within lysosomes.

Biological function summary

Hexosaminidase A functions as part of the lysosomal enzyme complex partnering with the GM2 activator protein and another related enzyme Hexosaminidase B. The complex is critical in the catabolism of GM2 gangliosides a type of lipid found in cell membranes specifically in neuronal cell membranes. Efficient function of Hexosaminidase A prevents accumulation of the lipid ensuring cellular health particularly in neurons.

Pathways

Hexosaminidase A is significant within glycolipid metabolism pathways and lysosomal degradation pathways. It cooperates closely with enzymes like Hexosaminidase B and the GM2 activator protein within these processes. These pathways are important for the breakdown of gangliosides which prevents their accumulation and maintains cellular function in neuronal tissues.

HEXA mutations have a direct link to Tay-Sachs disease a severe genetic disorder affecting nerve cells. This disorder results from HEXA mutations causing deficient enzyme activity leading to GM2 ganglioside accumulation. Additionally Sandhoff disease links through similar pathways though its relation more involves Hexosaminidase B deficiencies. Both disorders highlight the importance of a functioning Hexosaminidase A enzyme for normal neurological function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed : 11707436, PubMed : 8123671, PubMed : 8672428, PubMed : 9694901). The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2 (PubMed : 11707436). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed : 11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed : 8123671, PubMed : 8672428, PubMed : 9694901).

See full target information HEXA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 54: PubMed39219277

2024

TREM2, a critical activator of pyroptosis, mediates the anti‑tumor effects of piceatannol in uveal melanoma cells via caspase 3/GSDME pathway

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Jiu,Wenjie Li,Yang Liu,Lin Liu,Hong Lu

Nature communications 12:1453 PubMed33674603

2021

Identification of CD318, TSPAN8 and CD66c as target candidates for CAR T cell based immunotherapy of pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Schäfer,Stefan Tomiuk,Laura N Küster,Wa'el Al Rawashdeh,Janina Henze,German Tischler-Höhle,David J Agorku,Janina Brauner,Cathrin Linnartz,Dominik Lock,Andrew Kaiser,Christoph Herbel,Dominik Eckardt,Melina Lamorte,Dorothee Lenhard,Julia Schüler,Philipp Ströbel,Jeannine Missbach-Guentner,Diana Pinkert-Leetsch,Frauke Alves,Andreas Bosio,Olaf Hardt

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HEXA antibody

Flow Cytometry (Intracellular) - Anti-HEXA antibody (AB230488)

Western blot - Anti-HEXA antibody (AB230488)

Western blot - Anti-HEXA antibody (AB230488)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXA antibody (AB230488)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise