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AB189865

Anti-HEXA antibody - C-terminal

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(4 Publications)

Rabbit Polyclonal HEXA antibody. C-terminal. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HEXA aa 250 to C-terminus.

View Alternative Names

Beta-hexosaminidase subunit alpha, Beta-N-acetylhexosaminidase subunit alpha, N-acetyl-beta-glucosaminidase subunit alpha, Hexosaminidase subunit A, HEXA

1 Images
Western blot - Anti-HEXA antibody - C-terminal (AB189865)
  • WB

Supplier Data

Western blot - Anti-HEXA antibody - C-terminal (AB189865)

Blocking buffer : 3% nonfat dry milk in TBST.

All lanes:

Western blot - Anti-HEXA antibody - C-terminal (ab189865) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate at 25 µg

Lane 2:

BT-474 cell lysate at 25 µg

Lane 3:

SW620 cell lysate at 25 µg

Lane 4:

HepG2 cell lysate at 25 µg

Lane 5:

HT-29 cell lysate at 25 µg

Lane 6:

Mouse testis tissue lysate at 25 µg

Lane 7:

Mouse brain tissue lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L)

Predicted band size: 61 kDa

true

Exposure time: 5s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human HEXA aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P06865

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HEXA gene codes for the enzyme Hexosaminidase A also known as hexosaminidase alpha or HEXA subunit. This enzyme has several subunits with a molecular mass of approximately 58 kDa. HEXA expresses in various tissues but it shows high expression in the brain and other neural tissues. Mechanically Hexosaminidase A is involved in the hydrolysis of GM2 gangliosides into GM3 by removing the N-acetylgalactosamine residue which plays a role in the degradation of glycolipids within lysosomes.
Biological function summary

Hexosaminidase A functions as part of the lysosomal enzyme complex partnering with the GM2 activator protein and another related enzyme Hexosaminidase B. The complex is critical in the catabolism of GM2 gangliosides a type of lipid found in cell membranes specifically in neuronal cell membranes. Efficient function of Hexosaminidase A prevents accumulation of the lipid ensuring cellular health particularly in neurons.

Pathways

Hexosaminidase A is significant within glycolipid metabolism pathways and lysosomal degradation pathways. It cooperates closely with enzymes like Hexosaminidase B and the GM2 activator protein within these processes. These pathways are important for the breakdown of gangliosides which prevents their accumulation and maintains cellular function in neuronal tissues.

HEXA mutations have a direct link to Tay-Sachs disease a severe genetic disorder affecting nerve cells. This disorder results from HEXA mutations causing deficient enzyme activity leading to GM2 ganglioside accumulation. Additionally Sandhoff disease links through similar pathways though its relation more involves Hexosaminidase B deficiencies. Both disorders highlight the importance of a functioning Hexosaminidase A enzyme for normal neurological function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed : 11707436, PubMed : 8123671, PubMed : 8672428, PubMed : 9694901). The isozyme S is as active as the isozyme A on the anionic bis-sulfated glycans, the chondroitin-6-sulfate trisaccharide (C6S-3), and the dermatan sulfate pentasaccharide, and the sulfated glycosphingolipid SM2 (PubMed : 11707436). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed : 11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed : 8123671, PubMed : 8672428, PubMed : 9694901).
See full target information HEXA
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 25:77 PubMed39901081

2025

HACE1 protects against myocardial ischemia-reperfusion injury via inhibition of mitochondrial fission in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bang-Xia Liu,Juan Zheng,Zhan-Wei Tang,Lei Gao,Meng Wang,Ying Sun,Chen Chen,Heng-Chen Yao

Frontiers in molecular neuroscience 16:1242814 PubMed38098938

2023

Characterization of a phenotypically severe animal model for human AB-Variant GM2 gangliosidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie M Deschenes,Camilyn Cheng,Prem Khanal,Brianna M Quinville,Alex E Ryckman,Melissa Mitchell,Alexey V Pshezhetsky,Jagdeep S Walia

Nature communications 14:3911 PubMed37400440

2023

Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation.

Applications

Unspecified application

Species

Unspecified reactive species

Alessia Calcagni',Leopoldo Staiano,Nicolina Zampelli,Nadia Minopoli,Niculin J Herz,Giuseppe Di Tullio,Tuong Huynh,Jlenia Monfregola,Alessandra Esposito,Carmine Cirillo,Aleksandar Bajic,Mahla Zahabiyon,Rachel Curnock,Elena Polishchuk,Luke Parkitny,Diego Luis Medina,Nunzia Pastore,Peter J Cullen,Giancarlo Parenti,Maria Antonietta De Matteis,Paolo Grumati,Andrea Ballabio

Acta neuropathologica communications 11:70 PubMed37118844

2023

Patients with sporadic FTLD exhibit similar increases in lysosomal proteins and storage material as patients with FTD due to GRN mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Skylar E Davis,Anna K Cook,Justin A Hall,Yuliya Voskobiynyk,Nancy V Carullo,Nicholas R Boyle,Ahmad R Hakim,Kristian M Anderson,Kierra P Hobdy,Derian A Pugh,Charles F Murchison,Laura J McMeekin,Micah Simmons,Katherine A Margolies,Rita M Cowell,Alissa L Nana,Salvatore Spina,Lea T Grinberg,Bruce L Miller,William W Seeley,Andrew E Arrant
View all publications
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Product promise

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