JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB140649

Anti-HEXB antibody [EPR7978]

Be the first to review this product! Submit a review

|

(8 Publications)

Rabbit Recombinant Monoclonal HEXB antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.

View Alternative Names

HCC7, HEXB, Beta-hexosaminidase subunit beta, Beta-N-acetylhexosaminidase subunit beta, Cervical cancer proto-oncogene 7 protein, N-acetyl-beta-glucosaminidase subunit beta, Hexosaminidase subunit B, HCC-7

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (AB140649)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (AB140649)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HEXB with Purified ab140649 at 1 : 500 dilution (4.84 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (AB140649)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] (AB140649)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HEXB with ab140649 at a 1/25 dilution.

This image was generated using the unpurified version of the product.

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)
  • WB

Lab

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : HEXB knockout HAP1 whole cell lysate (20 μg)
Lane 3 : Jurkat whole cell lysate (20 μg)
Lane 4 : HeLa whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab140649 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab140649 was shown to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. ab140649 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (ab140649)

Predicted band size: 63 kDa

false

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)
  • WB

Unknown

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)
  • WB

Lab

Western blot - Anti-HEXB antibody [EPR7978] (AB140649)

The double bands caused by proteolytic processing are consistent with what has been described in PMID 2139028

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (ab140649) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 29 kDa,63 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7978

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/500", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target known as HEXB also called Hexosaminidase subunit beta consists of protein subunit that helps in the formation of the enzyme beta-hexosaminidase. This enzyme is active in lysosomes and it weighs about 63 kDa. Beta-hexosaminidase functions to break down GM2 gangliosides which are molecules in cell membranes. HEXB expresses mainly in the brain and liver tissues signaling its role in neurological and hepatic processes.
Biological function summary

The beta-hexosaminidase enzyme formed by HEXB participates in the lysosomal degradation pathway. This enzyme requires formation of a complex with its counterpart HEXA to become biologically active. Together they catalyze the hydrolysis of GM2 gangliosides into GM3 gangliosides which is an essential step for cell membrane metabolism. This process is important for maintaining cellular homeostasis and preventing accumulation of toxic substances within cells.

Pathways

The HEXB encoded enzyme participates in sphingolipid metabolism an integral component of lipid metabolic pathways. Sphingolipids are part of many cellular functions including signal transmission and cell recognition. HEXB through the lysosomal degradation of gangliosides works closely with HEXA and NEU1 which further processes GM3 gangliosides into simpler molecules. Such interactions underline HEXB’s significant role in maintaining the balance of lipid metabolism within cells.

Mutations or deficiencies in HEXB lead to serious genetic conditions such as Sandhoff disease. This disorder results from the improper breakdown of gangliosides due to the lack of functional HEXB leading to their accumulation which severely damages nerve cells and results in neurological impairments. The partnership between HEXB and HEXA emphasizes the importance of both subunits as malfunction in either can contribute to disease progression by disrupting normal ganglioside metabolism.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides (PubMed : 11707436, PubMed : 8123671, PubMed : 8672428, PubMed : 9694901). The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide (PubMed : 11707436). Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A (PubMed : 8123671, PubMed : 8672428, PubMed : 9694901). During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida (By similarity).
See full target information HEXB
style
left-column

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Medical sciences (Basel, Switzerland) 13: PubMed40981193

2025

Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23.

Applications

Unspecified application

Species

Unspecified reactive species

Andrey Kostin,Alexei Lyundup,Alexander Alekhnovich,Aleksandra Prikhodko,Olga Patsap,Sofia Gronskaia,Zhanna Belaya,Olga Lesnyak,Galina Melnichenko,Natalia Mokrysheva,Igor Buchwalow,Markus Tiemann,Dmitrii Atiakshin

iScience 26:107919 PubMed37822503

2023

A quinolin-8-ol sub-millimolar inhibitor of UGGT, the ER glycoprotein folding quality control checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin P Guay,Roberta Ibba,J L Kiappes,Snežana Vasiljević,Francesco Bonì,Maria De Benedictis,Ilaria Zeni,James D Le Cornu,Mario Hensen,Anu V Chandran,Anastassia L Kantsadi,Alessandro T Caputo,Juan I Blanco Capurro,Yusupha Bayo,Johan C Hill,Kieran Hudson,Andrea Lia,Juliane Brun,Stephen G Withers,Marcelo Martí,Emiliano Biasini,Angelo Santino,Matteo De Rosa,Mario Milani,Carlos P Modenutti,Daniel N Hebert,Nicole Zitzmann,Pietro Roversi

Nature medicine 28:251-259 PubMed35145305

2022

AAV gene therapy for Tay-Sachs disease.

Applications

Unspecified application

Species

Unspecified reactive species

Terence R Flotte,Oguz Cataltepe,Ajit Puri,Ana Rita Batista,Richard Moser,Diane McKenna-Yasek,Catherine Douthwright,Gwladys Gernoux,Meghan Blackwood,Christian Mueller,Phillip W L Tai,Xuntian Jiang,Scot Bateman,Spiro G Spanakis,Julia Parzych,Allison M Keeler,Aly Abayazeed,Saurabh Rohatgi,Laura Gibson,Robert Finberg,Bruce A Barton,Zeynep Vardar,Mohammed Salman Shazeeb,Matthew Gounis,Cynthia J Tifft,Florian S Eichler,Robert H Brown,Douglas R Martin,Heather L Gray-Edwards,Miguel Sena-Esteves

BBA advances 2:100032 PubMed37082581

2021

Increased phosphorylation of HexM improves lysosomal uptake and potential for managing GM2 gangliosidoses.

Applications

Unspecified application

Species

Unspecified reactive species

Graeme Benzie,Kristen Bouma,Taylor Battellino,Steven Cooper,Rick Hemming,Wafa Kammouni,Lin Liu,Cuong Do,Mazdak Khajehpour,Helene Perreault,Stuart Kornfeld,Barbara Triggs-Raine,Brian L Mark

eLife 9: PubMed33320095

2020

Quantitative glycoproteomics reveals cellular substrate selectivity of the ER protein quality control sensors UGGT1 and UGGT2.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin M Adams,Nathan P Canniff,Kevin P Guay,Ida Signe Bohse Larsen,Daniel N Hebert

The Journal of cell biology 218:615-631 PubMed30559172

2018

Retromer has a selective function in cargo sorting via endosome transport carriers.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Cui,Julian M Carosi,Zhe Yang,Nicholas Ariotti,Markus C Kerr,Robert G Parton,Timothy J Sargeant,Rohan D Teasdale

Oncotarget 8:91199-91208 PubMed29207636

2017

Characterization of urinary extracellular vesicle proteins in muscle-invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher R Silvers,Hiroshi Miyamoto,Edward M Messing,George J Netto,Yi-Fen Lee

Journal of human genetics 58:611-7 PubMed23759947

2013

Molecular pathology of Sandhoff disease with p.Arg505Gln in HEXB: application of simulation analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Naoko Yasui,Yutaka Takaoka,Hisahide Nishio,Dian K Nurputra,Kenji Sekiguchi,Hirotoshi Hamaguchi,Hisatomo Kowa,Eiichi Maeda,Aki Sugano,Kenji Miura,Toshiyuki Sakaeda,Fumio Kanda,Tatsushi Toda
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB75852

Anti-Cathepsin D antibody [EPR3057Y]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-Cathepsin D antibody [EPR3057Y] (AB75852)

  • 5
  • 11
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB18528

Anti-LAMP2A antibody - Lysosome Marker

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2A antibody - Lysosome Marker (AB18528)

  • 4
  • 15
Primary Antibodies

AB190684

Anti-SorLA/SORL1 antibody [EPR14670]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SorLA/SORL1 antibody [EPR14670] (AB190684)

  • 1
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com