Anti-HEXB antibody [EPR7978] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (AB248929)

This data was developed using ab140649, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer tissue sections labeling HEXB with Purified ab140649 at 1 : 500 dilution (4.84 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HEXB antibody [EPR7978] - BSA and Azide free (AB248929)

This data was developed using ab140649, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling HEXB with ab140649 at a 1/25 dilution.

This image was generated using the unpurified version of the product.

• WB

Lab

Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (AB248929)

This data was developed using ab140649, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)

Lane 2 : HEXB knockout HAP1 whole cell lysate (20 μg)

Lane 3 : Jurkat whole cell lysate (20 μg)

Lane 4 : HeLa whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab140649 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab140649 was shown to specifically react with HEXB when HEXB knockout samples were used. Wild-type and HEXB knockout samples were subjected to SDS-PAGE. ab140649 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) ab216773 and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (<a href='/en-us/products/primary-antibodies/hexb-antibody-epr7978-ab140649'>ab140649</a>)

Predicted band size: 63 kDa

false

• WB

Unknown

Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (AB248929)

This data was developed using ab140649, the same antibody clone in a different buffer formulation.

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (<a href='/en-us/products/primary-antibodies/hexb-antibody-epr7978-ab140649'>ab140649</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

Caco-2 cell lysate at 10 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

false

• WB

Lab

Western blot - Anti-HEXB antibody [EPR7978] - BSA and Azide free (AB248929)

This data was developed using ab140649, the same antibody clone in a different buffer formulation.

The double bands caused by proteolytic processing are consistent with what has been described in PMID : 2139028.

All lanes:

Western blot - Anti-HEXB antibody [EPR7978] (<a href='/en-us/products/primary-antibodies/hexb-antibody-epr7978-ab140649'>ab140649</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 29 kDa,63 kDa

false