AB58624

Anti-HEXIM1 antibody [2416C2a]

Name: Anti-HEXIM1 antibody [2416C2a] (ab58624) | Abcam
Brand: Abcam Limited
SKU: AB58624
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal HEXIM1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HEXIM1.

View Alternative Names

CLP1, EDG1, HIS1, MAQ1, HEXIM1, Protein HEXIM1, Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein, Hexamethylene bis-acetamide-inducible protein 1, Menage a quatre protein 1

1 Images

Unknown

Western blot - Anti-HEXIM1 antibody [2416C2a] (AB58624)

The MW is low because the recombinant fragment used as immunogen, rather than the full length protein, was used as positive control

All lanes:

Western blot - Anti-HEXIM1 antibody [2416C2a] (ab58624)

All lanes:

immunogen (recombinant pfragment)

Predicted band size: 41 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2416C2a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human HEXIM1. The exact immunogen used to generate this antibody is proprietary information.

O94992

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEXIM1 also known as hexamethylene bis-acetamide inducible protein 1 is a protein with an approximate molecular mass of 49 kDa. It predominantly resides in the nucleus and is ubiquitously expressed across various tissues. This protein functions primarily in the regulation of transcription by modulating the activity of P-TEFb (Positive Transcription Elongation Factor b). HEXIM1 acts as an inhibitor by binding to the cyclin T1 and CDK9 components of P-TEFb preventing its role in promoting RNA polymerase II activity during transcription elongation.

Biological function summary

HEXIM1 serves a critical regulatory role within the transcription machinery in cells. It acts as an essential component of the HEXIM1/P-TEFb/7SK snRNP complex which helps maintain transcriptional homeostasis. This complex involves 7SK small nuclear RNA which makes the transcription process more efficient by sequestering P-TEFb in an inactive state. By doing this HEXIM1 controls the release and activation of P-TEFb ensuring accurate transcription regulation and preventing aberrant gene expression.

Pathways

HEXIM1 is an integral element in managing transcription elongation processes. It is known to be part of significant signaling pathways such as the P-TEFb dependent transcription pathway. HEXIM1 interacts with proteins like CDK9 and cyclin T1 that are fundamental to its regulatory function. By controlling these interactions HEXIM1 influences RNA polymerase II's transition into productive elongation mode essential for proper gene expression.

Scientists have associated dysregulation of HEXIM1 activity with several conditions including cancer and cardiac hypertrophy. Aberrant HEXIM1 function or expression can lead to unchecked cellular proliferation in cancer due in part to misregulated P-TEFb activity. Moreover disruptions in this protein's regulatory capability have links to cardiac disorders where related proteins like CDK9 play roles in pathological cardiac growth. Therefore HEXIM1 serves as a potential target for therapeutic interventions concerning transcriptional dysregulation-related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor (PubMed : 14580347, PubMed : 15201869, PubMed : 15713661). Core component of the 7SK RNP complex : in cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed : 12832472, PubMed : 14580347, PubMed : 15201869, PubMed : 15713661). May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity (PubMed : 15940264, PubMed : 15941832, PubMed : 17088550). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed : 28712728).

See full target information HEXIM1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Orphanet journal of rare diseases 20:453 PubMed40849475

2025

Alpha-lipoic acid supplementation improves pathological alterations in cellular models of Friedreich ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Talaverón-Rey,Diana Reche-López,Suleva Povea-Cabello,Mónica Álvarez-Córdoba,David Gómez-Fernández,Ana Romero-González,Paula Cilleros-Holgado,José Manuel Romero-Domínguez,Alejandra López-Cabrera,Rocío Piñero-Pérez,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HEXIM1 antibody [2416C2a]

Western blot - Anti-HEXIM1 antibody [2416C2a] (AB58624)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise