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AB127710

Anti-HEXIM2 antibody

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(1 Publication)

Rabbit Polyclonal HEXIM2 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HEXIM2 aa 1 to C-terminus.

View Alternative Names

L3, HEXIM2, Protein HEXIM2, Hexamethylene bis-acetamide-inducible protein 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-HEXIM2 antibody (AB127710)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HEXIM2 antibody (AB127710)

ab12710, at 1/500 dilution, staining HEXIM2 in Paraformaldehyde-fixed HeLa cells by Immunofluorescence (green). Alpha-tubulin filaments (Red).

Western blot - Anti-HEXIM2 antibody (AB127710)
  • WB

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Western blot - Anti-HEXIM2 antibody (AB127710)

12% SDS PAGE

All lanes:

Western blot - Anti-HEXIM2 antibody (ab127710) at 1/500 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

HepG2 whole cell lysate at 30 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human HEXIM2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96MH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HEXIM2 also known as Hexamethylene Bisacetamide-Inducible Protein 2 is a protein with a molecular mass of approximately 36 kDa. It functions as a regulatory protein with roles in transcription modulation. HEXIM2 is expressed in various human tissues with notable expression levels in the heart and skeletal muscle. This protein acts as a part of the regulation network that interacts with RNA polymerase II during the transcription process.
Biological function summary

HEXIM2 plays a role in the inhibitory regulation of transcription. It forms part of the 7SK small nuclear ribonucleoprotein (snRNP) complex which is known for controlling the activity of the positive transcription elongation factor b (P-TEFb). This complex regulates RNA polymerase II by inhibiting its transcriptional elongation capability therefore controlling gene expression. The presence of HEXIM2 in this complex indicates its involvement in modulating gene expression through RNA interference pathways.

Pathways

HEXIM2 operates within the transcription elongation regulation pathway and the cell cycle control pathway. In the transcription elongation regulation pathway it contributes significantly to the inhibition of P-TEFb which associates with Cyclin T1 and CDK9. These proteins are vital for allowing RNA polymerase II to proceed with transcription. HEXIM2's involvement ensures the proper timing and modulation of transcriptional activities controlling cellular functions and responses.

HEXIM2 has associations with cancer and cardiac-related conditions. Its role in transcription regulation links it to various forms of cancer where aberrant gene expression is a hallmark. Additionally HEXIM2's expression in cardiac tissues suggests implications in cardiac disorders. Interactions with proteins like Cyclin T1 and CDK9 which are also involved in these conditions highlight potential pathways where HEXIM2 might contribute to disease progression or resistance mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor (PubMed : 15713661, PubMed : 15713662). Core component of the 7SK RNP complex : in cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation (PubMed : 15713661, PubMed : 15713662).
See full target information HEXIM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:1106-1114 PubMed40109092

2025

METTL3-mediated m A modification of pri-miRNA-31 promotes hypertrophic scar progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qirui Wang,Jialin Hou,Siyi Zeng,Xue Wang,Yimin Liang,Renpeng Zhou
View all publications
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