Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric) (AB317788)

This image was produced using ab317798, the same antibody clone but in a different formulation.

ab317798 staining Hexokinase-1 in MCF7 (positive) and HepG2 (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab317798 at 1µg/ml and ab185031, Alexa Fluor® 488 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker. Cells were then incubated with ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 4% paraformaldehyde (10 min).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric) (AB317788)

Immunofluorescence staining of hexokinase 1 in sections of formalin-fixed paraffin-embedded human stomach (positive) and human pancreas (negative). This image was produced using ab317798, the same antibody clone but in a different formulation. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317798 at 1/100 dilution, and then incubated for 1 hour with ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.