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Mouse Monoclonal Hexokinase 1 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317798), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (AB317798), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

13 products for Alternative Version

    Target data

    Function

    Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).

    Alternative names

    Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

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    Mouse Monoclonal Hexokinase 1 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human samples.

    Alternative names

    Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

    Key facts

    Isotype

    IgG1

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR10134(B)

    Purification technique

    Affinity purification Protein A

    Concentration
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    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Notes

    ab317798 is the carrier-free version of Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial OMM – Mouse IgG1 (Chimeric) ab317788.

    This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-Hexokinase 1 antibody [EPR10134(B)] - Mitochondrial Outer Membrane Marker ab150423). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    2 product images

    • Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)

      ab317798 staining Hexokinase-1 in MCF7 (positive) and HepG2 (negative) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab317798 at 1µg/ml and Alexa Fluor® 488 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab185031, Alexa Fluor® 488 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase 1 antibody [EPR10134(B)] – Mouse IgG1 (Chimeric) – BSA and Azide Free (ab317798)

      Immunofluorescence staining of hexokinase 1 in sections of formalin-fixed paraffin-embedded human stomach (positive) and human pancreas (negative).

      Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317798 at 1/100 dilution, and then incubated for 1 hour with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com