Rabbit Recombinant Monoclonal Hexokinase 1 antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img7089.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)")
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img2394.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)")
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img68908.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
Alternative names
Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1
Recommended products
Rabbit Recombinant Monoclonal Hexokinase 1 antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR10135(B)
- Purity
Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img7089.jpg "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)")
Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)
Lanes 1-4: Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HK1 (Hexokinase 1) knockout HEK-293T cell line ab267279 (knockout cell lysate Human HK1 (Hexokinase 1) knockout HEK-293T cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HK1 knockout HeLa cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img2394.jpg "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)")
Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)
Lanes 1-4: Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human HK1 (Hexokinase 1) knockout HEK-293T cell line ab267279 (knockout cell lysate Human HK1 (Hexokinase 1) knockout HEK-293T cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution
Lane 1: Wild-type HEK293T cell lysate at 20 µg
Lane 2: HK1 knockout HEK293T cell lysate at 20 µg
Lane 3: MCF7 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
![Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-1-antibody-epr10135b-ab154839--western-blot-img68908.jpg "Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)")
Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839)
All lanes: Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: MCF7 cell lysate at 10 µg
Lane 3: U-87 MG cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Predicted band size: 102 kDa
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