AB154839

Anti-Hexokinase 1 antibody [EPR10135(B)]

RabMAb
Recombinant
KO Validated
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(6 Publications)

Rabbit Recombinant Monoclonal Hexokinase 1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

3 Images

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Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

Lanes 1-4 : Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267279 (knockout cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HK1 knockout HEK293T cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 102 kDa

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Lab

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

Lanes 1-4 : Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.

All lanes:

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

HK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human HK1 (Hexokinase 1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hk1-hexokinase-1-knockout-hek-293t-cell-line-ab267279'>ab267279</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

Unknown

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

All lanes:

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

U-87 MG cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 102 kDa

false

Carrier free

Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10135(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed : 1637300, PubMed : 25316723, PubMed : 27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed : 27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed : 27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed : 27374331).

See full target information HK1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular neuroscience 19:1603657 PubMed40958776

2025

APOE genotype-dependent differences in human astrocytic energy metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Budny,Chantal Bodenmann,Kathrin J Zürcher,Maik Krüger,Sherida M de Leeuw,Rebecca Z Weber,Ruslan Rust,Luca Ravotto,Iván Ruminot,L Felipe Barros,Bruno Weber,Christian Tackenberg

Cancer biology & therapy 26:2546219 PubMed40808274

2025

Hypoxia-induced PYCR1 regulates glycolysis and histone lactylation to promote bladder cancer progression and metastasis via SLC6A14/Glutamine metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Qinghua Jiang,Quan Yang,Yujie Zhou,Jiansong Wang

Heliyon 10:e37063 PubMed39296221

2024

Deciphering the role of PLCD3 in lung cancer: A gateway to glycolytic reprogramming via PKC-Rap1 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Mingjiang Li,Xiaoping Li,Ting Xiao,Honggang Zhou,Weidong Zhang,Ping Wang

Translational cancer research 13:4205-4218 PubMed39262496

2024

The role of chromatin modulator DPY30 in glucose metabolism of colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Mao,Shiqin Yang,Ye Zhang,Huajun Yang,Danhong Yan,Lingzhi Zhang

International journal of oncology 63: PubMed37293856

2023

Exosomes from PYCR1 knockdown bone marrow mesenchymal stem inhibits aerobic glycolysis and the growth of bladder cancer cells via regulation of the EGFR/PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Ying Jiang,Jian Liu,Huifeng Fu,Quan Yang,Wei Song,Yuanwei Li

The Journal of neuroscience : the official journal : PubMed33478991

2021

Tuba activates Cdc42 during neuronal polarization downstream of the small GTPase Rab8a.

Applications

Unspecified application

Species

Unspecified reactive species

Pamela J Urrutia,Felipe Bodaleo,Daniel A Bórquez,Yuta Homma,Victoria Rozes-Salvador,Cristopher Villablanca,Cecilia Conde,Mitsunori Fukuda,Christian González-Billault

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hexokinase 1 antibody [EPR10135(B)]

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (AB154839)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (6)

Product promise