Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (AB249143)

This data was developed using the same antibody clone in a different buffer formulation (ab154839).

Lanes 1-4 : Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267279 (knockout cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] (<a href='/en-us/products/primary-antibodies/hexokinase-1-antibody-epr10135b-ab154839'>ab154839</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

HK1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human HK1 (Hexokinase 1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hk1-hexokinase-1-knockout-hek-293t-cell-line-ab267279'>ab267279</a>)

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 102 kDa

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