AB65069

Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker

Name: Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) | Abcam
Brand: Abcam Limited
SKU: AB65069
Rating: 5 (5 reviews)

(5 Reviews)

(7 Publications)

Rabbit Polyclonal Hexokinase 1 antibody. Mitochondrial Outer Membrane marker. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human HK1.

View Alternative Names

Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1

2 Images

Unknown

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (AB65069)

All lanes:

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution

Lane 1:

extracts from HeLa cells at 5 µg

Lane 2:

extracts from HeLa cells at 5 µg with immunizing peptide

Predicted band size: 102 kDa

Observed band size: 102 kDa,60 kDa

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Unknown

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (AB65069)

All lanes:

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution

All lanes:

Western blot - Recombinant human Hexokinase 1 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-hexokinase-1-protein-ab85918'>ab85918</a>) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution

Predicted band size: 102 kDa

true

Exposure time: 4min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human HK1. The exact immunogen used to generate this antibody is proprietary information.

P19367

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed : 1637300, PubMed : 25316723, PubMed : 27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed : 27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed : 27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed : 27374331).

See full target information HK1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International heart journal 64:274-282 PubMed37005321

2023

Silencing of Hsa_circ_0055440 Alleviates Hypoxia-Induced Cardiomyocyte Injury by Regulating the MiR-499b-5p/ACSL1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lianhua Yan,Haijun Qi,Wei Zhou

Journal of assisted reproduction and genetics 40:343-359 PubMed36593322

2023

Granulosa cells from immature follicles exhibit restricted glycolysis and reduced energy production: a dominant problem in polycystic ovary syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Sahar Mazloomi,Marzieh Sanoee Farimani,Heidar Tavilani,Jamshid Karimi,Iraj Amiri,Ebrahim Abbasi,Iraj Khodadadi

Journal of neuroinflammation 17:153 PubMed32386505

2020

Immuno-metabolic impact of the multiple sclerosis patients' sera on endothelial cells of the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

M H Sheikh,S M Henson,R A Loiola,S Mercurio,A Colamatteo,G T Maniscalco,V De Rosa,S McArthur,E Solito

JCI insight 5: PubMed32134397

2020

Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.

Applications

Unspecified application

Species

Unspecified reactive species

Jinpeng Li,Haijie Liu,Susumu Takagi,Kyoko Nitta,Munehiro Kitada,Swayam Prakash Srivastava,Yuta Takagaki,Keizo Kanasaki,Daisuke Koya

Immunity 47:875-889.e10 PubMed29166588

2017

Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Madhav Kishore,Kenneth C P Cheung,Hongmei Fu,Fabrizia Bonacina,Guosu Wang,David Coe,Eleanor J Ward,Alessandra Colamatteo,Maryam Jangani,Andrea Baragetti,Giuseppe Matarese,David M Smith,Robert Haas,Claudio Mauro,David C Wraith,Klaus Okkenhaug,Alberico L Catapano,Veronica De Rosa,Giuseppe D Norata,Federica M Marelli-Berg

Journal of experimental & clinical cancer research 36:110 PubMed28810896

2017

Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Yifan Sun,Jingjing Li,Yan Wang,Yuxing Zhu,Yong Shi,Xiaojun Fan,Jianda Zhou,Ying Bao,Jie Xiao,Ke Cao,Peiguo Cao

EJNMMI research 4:34 PubMed26116108

2014

Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches.

Applications

IHC

Species

Unspecified reactive species

Tetyana Tegnebratt,Elisabeth Ruge,Sabine Bader,Nobuya Ishii,Satoshi Aida,Yasushi Yoshimura,Chia-Huey Ooi,Li Lu,Nicholas Mitsios,Valerie Meresse,Jan Mulder,Michael Pawlak,Miro Venturi,Jean Tessier,Sharon Stone-Elander

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (AB65069)

Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (AB65069)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

Product promise