Rabbit Polyclonal Hexokinase 1 antibody. Mitochondrial Outer Membrane marker. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human HK1.
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- Journal of neuroinflammation 17:1532020Immuno-metabolic impact of the multiple sclerosis patients' sera on endothelial cells of the blood-brain barrier.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM H Sheikh,S M Henson,R A Loiola,S Mercurio,A Colamatteo,G T Maniscalco,V De Rosa,S McArthur,E SolitoPubMed 32386505
- JCI insight 5:2020Renal protective effects of empagliflozin via inhibition of EMT and aberrant glycolysis in proximal tubules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinpeng Li et. al.PubMed 32134397
- Immunity 47:875-889.e102017Regulatory T Cell Migration Is Dependent on Glucokinase-Mediated Glycolysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadhav Kishore,Kenneth C P Cheung,Hongmei Fu,Fabrizia Bonacina,Guosu Wang,David Coe,Eleanor J Ward,Alessandra Colamatteo,Maryam Jangani,Andrea Baragetti,Giuseppe Matarese,David M Smith,Robert Haas,Claudio Mauro,David C Wraith,Klaus Okkenhaug,Alberico L Catapano,Veronica De Rosa,Giuseppe D Norata,Federica M Marelli-BergPubMed 29166588
- Journal of experimental & clinical cancer research 36:1102017Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Yu et. al.PubMed 28810896
- EJNMMI research 4:342014Evaluation of efficacy of a new MEK inhibitor, RO4987655, in human tumor xenografts by [(18)F] FDG-PET imaging combined with proteomic approaches.Applications:IHCReactive species:Unspecified reactive speciesTetyana Tegnebratt et. al.PubMed 26116108
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human HK1. The exact immunogen used to generate this antibody is proprietary information. Database link P19367
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
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Target data
Function
Catalyzes the phosphorylation of various hexoses, such as D-glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6-phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
Alternative names
Hexokinase-1, Brain form hexokinase, Hexokinase type I, Hexokinase-A, HK I, HK1
Recommended products
Rabbit Polyclonal Hexokinase 1 antibody. Mitochondrial Outer Membrane marker. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human HK1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human HK1. The exact immunogen used to generate this antibody is proprietary information. Database link P19367
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Stable for 12 months at -20°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069)
All lanes: Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution
All lanes: extracts from HeLa cells at 5 µg
Predicted band size: 102 kDa
Observed band size: 102 kDa, 60 kDa
Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069)
All lanes: Western blot - Anti-Hexokinase 1 antibody - Mitochondrial Outer Membrane Marker (ab65069) at 1/500 dilution
All lanes: Western blot - Recombinant human Hexokinase 1 protein (Recombinant human Hexokinase 1 protein ab85918) at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Exposure time: 4min
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