Mouse Monoclonal Hexokinase II antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 71 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HK2.
Images
![Western blot - Anti-Hexokinase II antibody [3D3] (AB104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--western-blot-img101246.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase II antibody [3D3] (AB104836)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (AB104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19048.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (AB104836)")
![Western blot - Anti-Hexokinase II antibody [3D3] (AB104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--western-blot-img116780.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase II antibody [3D3] (AB104836)")
Publications
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- BMC biology 21:982023Osteosarcoma tumors maintain intra-tumoral transcriptional heterogeneity during bone and lung colonization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanjana Rajan,Emily M Franz,Camille A McAloney,Tatyana A Vetter,Maren Cam,Amy C Gross,Cenny Taslim,Meng Wang,Matthew V Cannon,Alexander Oles,Ryan D RobertsPubMed 37106386
- BioFactors (Oxford, England) 49:600-6112023Redox-metabolic reprogramming of skin in mice lacking functional Nrf2 under basal conditions and cold acclimation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamara Zakic,Sara Stojanovic,Aleksandra Jankovic,Aleksandra Korac,Vanja Pekovic-Vaughan,Bato KoracPubMed 36585756
- International journal of molecular sciences 23:20223-Bromopyruvate Suppresses the Malignant Phenotype of Vemurafenib-Resistant Melanoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrik da Silva Vital,Murilo Bonatelli,Marina Pereira Dias,Larissa Vedovato Vilela de Salis,Mariana Tomazini Pinto,Fátima Baltazar,Silvya Stuchi Maria-Engler,Céline PinheiroPubMed 36555289
- Scientific reports 12:62612022The lactate sensor GPR81 regulates glycolysis and tumor growth of breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShota Ishihara,Kenji Hata,Katsutoshi Hirose,Tatsuo Okui,Satoru Toyosawa,Narikazu Uzawa,Riko Nishimura,Toshiyuki YonedaPubMed 35428832
- Bioengineered 13:9301-93112022HLA complex P5 upregulation is correlated with poor prognosis and tumor progression in esophageal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaqin Wang,Zhijun Yu,Weidong Shi,Jian Shen,Yun Guan,Feng NiPubMed 35389828
- Cell biology international 46:819-8282022CCL2 activates AKT signaling to promote glycolysis and chemoresistance in glioma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYafang Qian,Peng Ding,Jie Xu,Xiaohu Nie,Bin LuPubMed 35178826
- Cancers 14:2022Impact of Exogenous Treatment with Histidine on Hepatocellular Carcinoma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYusun Park,Yeonju Han,Dongwoo Kim,Sua Cho,WonJin Kim,Hyemin Hwang,Hye Won Lee,Dai Hoon Han,Kyung Sik Kim,Mijin Yun,Misu LeePubMed 35267513
- Cellular & molecular biology letters 27:172022Exosomal lncRNA TUG1 from cancer-associated fibroblasts promotes liver cancer cell migration, invasion, and glycolysis by regulating the miR-524-5p/SIX1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLe Lu,Jingjing Huang,Jiantao Mo,Xuanbo Da,Qiaoxin Li,Meng Fan,Hongwei LuPubMed 35193488
- Frontiers in cell and developmental biology 9:7791692022SIPA1 Enhances Aerobic Glycolysis Through HIF-2α Pathway to Promote Breast Cancer Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenguang Yao,Jun Weng,Lingyun Feng,Wanjun Zhang,Yan Xu,Peijing Zhang,Yoshimasa Tanaka,Li SuPubMed 35096814
- Biomedicines 9:2021Normalizing HIF-1α Signaling Improves Cellular Glucose Metabolism and Blocks the Pathological Pathways of Hyperglycemic Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarla Iacobini,Martina Vitale,Giuseppe Pugliese,Stefano MeniniPubMed 34572324
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.03% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- Recombinant Fragment Protein within Human HK2. The exact immunogen used to generate this antibody is proprietary information. Database link P52789
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
Alternative names
Hexokinase-2, Hexokinase type II, Hexokinase-B, Muscle form hexokinase, HK II, HK2
Recommended products
Mouse Monoclonal Hexokinase II antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 71 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HK2.
Key facts
- Isotype
IgG1
- Host species
Mouse
- Storage buffer
Preservative: 0.03% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human HK2. The exact immunogen used to generate this antibody is proprietary information. Database link P52789
- Clone number
3D3
- Purification technique
Affinity purification Protein G
- Concentration
- Purification notes
Purified from tissue culture supernatant.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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6 product images
![Western blot - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--western-blot-img101246.jpg "Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)")
Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
Lanes 1 - 4: Merged signal (red and green). Green - ab104836 observed at 102 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab104836 was shown to react with Hexokinase II in wild-type HEK-293T cells in western blot. The band observed in HK2 CRISPR/Cas9 edited sample lane below 102 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and HK2 CRISPR/Cas9 edited HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab104836 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1: Wild-type HEK-293T cell lysate at 20 µg
Lane 2: HK2 CRISPR/Cas9 edited HEK-293T cell lysate at 20 µg
Lane 3: HeLa cell lysate at 20 µg
Lane 4: A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19048.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)")
This image is courtesy of a customer review submitted by Ms Hadas PahimaImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
Immunohistochemical analysis of human skin tissue, staining Hexokinase II with ab104836.
Tissue was fixed with paraformaldehyde and blocked with 10% BSA for 2 hours at room temperature; antigen retrieval was by heat mediation in citrate buffer. Samples were incubated with primary antibody (1/250 in 5% NGS + 10% BSA in PBS) for 16 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/250) was used as the secondary antibody.![Western blot - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--western-blot-img116780.jpg "Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)")
Western blot - Anti-Hexokinase II antibody [3D3] (ab104836)
All lanes: Western blot - Anti-Hexokinase II antibody [3D3] (ab104836) at 1/500 dilution
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK293 cell lysate
Predicted band size: 102 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131714.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
ab104836 at 1/200 dilution staining Hexokinase II in human esophagus cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117781.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [3D3] (ab104836)
ab104836 at 1/200 dilution staining Hexokinase II in human lung cancer by Immunohistochemistry, Paraffin-embedded tissue with DAB staining.
![Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--flow-cytometry-img414943.jpg "Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)")
Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)
ab104836 staining Hexokinase II in K562 cells by Immunofluorescence (green) and negative control (purple).
![Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-3d3-ab104836--flow-cytometry-img414943.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Hexokinase II antibody [3D3] (ab104836)")
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