AB227198

Anti-Hexokinase II antibody

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(1 Review)

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(60 Publications)

Rabbit Polyclonal Hexokinase II antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 60 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HK2.

View Alternative Names

Hexokinase-2, Hexokinase type II, Hexokinase-B, Muscle form hexokinase, HK II, HK2

7 Images

Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody (AB227198)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody (AB227198)

Ice cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hexokinase II (green) using ab227198 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

Paraffin-embedded U87 (human) xenograft tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

Paraffin-embedded rat heart tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

Paraffin-embedded rat kidney tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

Paraffin-embedded mouse muscle tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (AB227198)

Paraffin-embedded rat stomach tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Hexokinase II antibody (AB227198)

WB

Supplier Data

Western blot - Anti-Hexokinase II antibody (AB227198)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 102 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

ICC/IF, IHC-P, WB

Immunogen

Recombinant Fragment Protein within Human HK2. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed : 23185017, PubMed : 26985301, PubMed : 29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed : 29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed : 18350175).

See full target information HK2

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Publications (60)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1712 PubMed41003853

2025

Hsa_circ_0075451 promotes NSCLC proliferation, metastasis, and glycolysis through interaction with the RNA-binding protein RBM4.

Applications

Unspecified application

Species

Unspecified reactive species

ChongYu Su,YuXuan Wang,Kang Shi,ZheXin Bai,DaPing Yu,ZhiDong Liu

CNS neuroscience & therapeutics 31:e70569 PubMed40927974

2025

Deer Antler S-Adenosylmethionine Ameliorates Depression-Like Behaviors and Neuroinflammation in CUMS Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjian Liu,Chenyang Han,Li Guo,Wenyan Li,Shasha Wu,Jian Sheng,Liping Zhai,Heping Shen,Genghuan Wang

Journal of translational medicine 23:923 PubMed40826409

2025

Metabolic interplay between endometrial cancer and tumor-associated macrophages: lactate-induced M2 polarization enhances tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodie Liu,Heming Sun,Jing Liang,Huan Yu,Ming Xue,Yuebo Li,Wenhui Li,Ying Xu,Bin Ling,Dingqing Feng

Journal of cell communication and signaling 19:e70034 PubMed40692714

2025

UBD-mediated glycolytic reprogramming promotes M2 macrophage polarization in ovarian cancer immune evasion.

Applications

Unspecified application

Species

Unspecified reactive species

Nana Zhang,Fengming Zhao,Hailong Chen,Juli Wang,Haiyan Li

Nature 644:759-768 PubMed40500435

2025

Early life high fructose impairs microglial phagocytosis and neurodevelopment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoquan Wang,Allie Lipshutz,Celia Martínez de la Torre,Alissa J Trzeciak,Zong-Lin Liu,Isabella C Miranda,Tomi Lazarov,Ana C Codo,Jesús E Romero-Pichardo,Achuth Nair,Tanya Schild,Waleska Saitz Rojas,Pedro H V Saavedra,Ann K Baako,Kelvin Fadojutimi,Michael S Downey,Frederic Geissmann,Giuseppe Faraco,Li Gan,Jon Iker Etchegaray,Christopher D Lucas,Marina Tanasova,Christopher N Parkhurst,Melody Y Zeng,Kayvan R Keshari,Justin S A Perry

Molecular carcinogenesis 64:552-564 PubMed39763297

2025

A-to-I-Edited miR-1304-3p Inhibits Glycolysis and Tumor Growth of Esophageal Squamous Cell Carcinoma by Inactivating Wnt5a/ROR2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Chen,Hang Zhou,Xian Yang,Yuzhen Zheng,Yujie Chen,Peiyuan Wang,Hao He,Shuoyan Liu,Feng Wang

Histology and histopathology 40:1275-1285 PubMed39679449

2024

Tumor necrosis factor receptor-associated protein 1 promotes aerobic glycolysis and cisplatin resistance by regulating the Wnt/β-catenin signaling pathway in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruijie Li,Mengguo Lv,Juan Liu,Qian Sun

Cancer & metabolism 12:32 PubMed39462429

2024

RHOF promotes Snail1 lactylation by enhancing PKM2-mediated glycolysis to induce pancreatic cancer cell endothelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Yanmin Yi,Han Liu,Jianwei Xu,Shuhai Chen,Dong Wu,Lei Wang,Feng Li

Biomolecules & biomedicine 25:82-93 PubMed39097833

2024

Salidroside exerts anti-tumor effects in ovarian cancer by inhibiting STAT3/c-Myc pathway-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Yu,Xiaoling Feng

Phytotherapy research : PTR 38:1799-1814 PubMed38330236

2024

Futoquinol improves Aβ-induced memory impairment in mice by inhibiting the activation of p38MAPK through the glycolysis pathway and regulating the composition of the gut microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhan Zhang,Hui Chen,Mengnan Zeng,Pengli Guo,Meng Liu,Bing Cao,Ru Wang,Fengxiao Hao,Xiaoke Zheng,Weisheng Feng

View all publications

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Product promise

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