Rabbit Polyclonal Hexokinase II antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Hexokinase-2.
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- Cell death & disease 14:8272023Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Zhang,Yong Zhong,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Bo Wu,Zhongwei LvPubMed 38092752
- Open medicine (Warsaw, Poland) 18:202306322023Hsa_circ_0028007 regulates the progression of nasopharyngeal carcinoma through the miR-1179/SQLE axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenya Li,Xiuwen Jiang,Lina ZhaoPubMed 37554147
- Cell death discovery 9:1332023PARP1 mediated PARylation contributes to myogenic progression and glucocorticoid transcriptional response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArnold Tan,Awais Z Younis,Alexander Evans,Jade V Creighton,Clare Coveny,David J Boocock,Craig Sale,Gareth G Lavery,Amanda S Coutts,Craig L DoigPubMed 37087471
- EMBO reports 24:e563252023METTL14 modulates glycolysis to inhibit colorectal tumorigenesis in p53-wild-type cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichao Hou,Xintian Zhang,Han Yao,Lidan Hou,Qingwei Zhang,Enwei Tao,Xiaoqiang Zhu,Shanshan Jiang,Yimeng Ren,Xialu Hong,Shiyuan Lu,Xiaoxu Leng,Yile Xie,Yaqi Gao,Yu Liang,Ting Zhong,Bohan Long,Jing-Yuan Fang,Xiangjun MengPubMed 36794620
- Proceedings of the National Academy of Sciences of the United States of America 120:e22091771202023Mitochondrial control of microglial phagocytosis by the translocator protein and hexokinase 2 in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren H Fairley,Kei Onn Lai,Jia Hui Wong,Wei Jing Chong,Anselm Salvatore Vincent,Giuseppe D'Agostino,Xiaoting Wu,Roshan R Naik,Anusha Jayaraman,Sarah R Langley,Christiane Ruedl,Anna M BarronPubMed 36787364
- Cell & bioscience 12:1832022mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo TangPubMed 36371321
- The journal of gene medicine 24:e34182022Circ_0008717 promotes renal cell carcinoma progression by upregulating FBXO17 via targeting miR-217.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyao Shen,Ming Jiang,Wen Deng,Xiaoqiang Liu,Junhui Xiong,Zhigang Zeng,Hong Zhao,Xiaochun Zeng,Bin FuPubMed 35357059
- Journal of orthopaedic surgery and research 17:4212022Circ_0136666 aggravates osteosarcoma development through mediating miR-1244/CEP55 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Gao,Nanwei Xu,Kaisong Miao,Gao Huang,Yong HuangPubMed 36109749
- Journal of experimental & clinical cancer research : CR 41:2532022Tumor associated macrophages-derived exosomes facilitate hepatocellular carcinoma malignance by transferring lncMMPA to tumor cells and activating glycolysis pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinghao Xu,Chenhao Zhou,Jialei Weng,Zhaoshuo Chen,Qiang Zhou,Jian Gao,Guoming Shi,Aiwu Ke,Ning Ren,Huichuan Sun,Yinghao ShenPubMed 35986343
- World journal of surgical oncology 20:1622022Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Ding,Yi Lu,Xinjie Xie,Lei Cao,Shiying ZhengPubMed 35598017
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Hexokinase-2. The exact immunogen used to generate this antibody is proprietary information. Database link P52789
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 - 1/20000 | Notes - |
Species Human | Dilution info 1/5000 - 1/20000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
Alternative names
Hexokinase-2, Hexokinase type II, Hexokinase-B, Muscle form hexokinase, HK II, HK2
Recommended products
Rabbit Polyclonal Hexokinase II antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Hexokinase-2.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Hexokinase-2. The exact immunogen used to generate this antibody is proprietary information. Database link P52789
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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8 product images
Western blot - Anti-Hexokinase II antibody (ab227198)
7.5% SDS PAGE
All lanes: Western blot - Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution
All lanes: Mouse brain tissue lysate at 20 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (ab227198)
Paraffin-embedded rat kidney tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody (ab227198)
Ice cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Hexokinase II (green) using ab227198 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Western blot - Anti-Hexokinase II antibody (ab227198)
7.5% SDS-PAGE
All lanes: Western blot - Anti-Hexokinase II antibody (ab227198) at 1/10000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg
Lane 3: IMR32 whole cell extract at 30 µg
Lane 4: SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg
Developed using the ECL technique.
Predicted band size: 102 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (ab227198)
Paraffin-embedded U87 (human) xenograft tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (ab227198)
Paraffin-embedded mouse muscle tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (ab227198)
Paraffin-embedded rat heart tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody (ab227198)
Paraffin-embedded rat stomach tissue stained for Hexokinase II with ab227198 at 1/500 dilution in immunohistochemical analysis.
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