Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Hexokinase II in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img109937.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25308.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819)")
![Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunoprecipitation-img2315.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819)")
![Western blot - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--western-blot-img2850.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819)")
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img113235.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (AB228819)")
Publications
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- JCI insight 8:2023T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid RabbPubMed 37166984
- Cell reports 34:1088632021Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Thompson,Katherine Cascino,Alvaro A Ordonez,Weiqiang Zhou,Ajay Vaghasia,Anne Hamacher-Brady,Nathan R Brady,Im-Hong Sun,Rulin Wang,Avi Z Rosenberg,Michael Delannoy,Richard Rothman,Katherine Fenstermacher,Lauren Sauer,Kathyrn Shaw-Saliba,Evan M Bloch,Andrew D Redd,Aaron A R Tobian,Maureen Horton,Kellie Smith,Andrew Pekosz,Franco R D'Alessio,Srinivasan Yegnasubramanian,Hongkai Ji,Andrea L Cox,Jonathan D PowellPubMed 33691089
- medRxiv : the preprint server for health sciences :2020Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Thompson,Katherine Cascino,Alvaro A Ordonez,Weiqiang Zhou,Ajay Vaghasia,Anne Hamacher-Brady,Nathan R Brady,Im-Hong Sun,Rulin Wang,Avi Z Rosenberg,Michael Delannoy,Richard Rothman,Katherine Fenstermacher,Lauren Sauer,Kathyrn Shaw-Saliba,Evan M Bloch,Andrew D Redd,Aaron Ar Tobian,Maureen Horton,Kellie Smith,Andrew Pekosz,Franco R D'Alessio,Srinivasan Yegnasubramanian,Hongkai Ji,Andrea L Cox,Jonathan D PowellPubMed 32935120
- Experimental and therapeutic medicine 20:572-5802020MicroRNA-513a-3p regulates colorectal cancer cell metabolism via targeting hexokinase 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Li et. al.PubMed 32537015
- Cell 179:1483-1498.e222019Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Cheih Chao,Cristina Gutiérrez-Vázquez,Veit Rothhammer,Lior Mayo,Michael A Wheeler,Emily C Tjon,Stephanie E J Zandee,Manon Blain,Kalil Alves de Lima,Maisa C Takenaka,Julian Avila-Pacheco,Patrick Hewson,Lei Liu,Liliana M Sanmarco,Davis M Borucki,Gabriel Z Lipof,Sunia A Trauger,Clary B Clish,Jack P Antel,Alexandre Prat,Francisco J QuintanaPubMed 31813625
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
| Recombinant full length protein - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Not recommended for rat and mouse tissues. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
2 products for Alternative Version
Target data
Function
Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:23185017, PubMed:26985301, PubMed:29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed:18350175).
Alternative names
Hexokinase-2, Hexokinase type II, Hexokinase-B, Muscle form hexokinase, HK II, HK2
Recommended products
Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Hexokinase II in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- KO validated for confirmed specificity
- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20839
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
What is this antibody validated in?
Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of Hexokinase II?
Anti-Hexokinase II [EPR20839] - BSA and Azide free (ab228819) specifically detects a band for Hexokinase II (UniProt: P52789) at a molecular weight of 102kDa.
Specificity confirmed
The specificity of Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) has been confirmed by Western blot testing in HK2 Knockout HEK-293 cell line.
Other related products
We have a range of other formats of antibody clone [EPR20839] also available for your convenience: Anti-Hexokinase II antibody [EPR20839] ab209847, Carrier free - ab228819, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] ab237314, HRP - HRP Anti-Hexokinase II antibody [EPR20839] ab237315, Anti-Hexokinase II antibody [EPR20839] – Mouse IgG1 (Chimeric) ab323242, Carrier free - Anti-Hexokinase II antibody [EPR20839] – Mouse IgG1 (Chimeric) – BSA and Azide Free ab323251
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img109937.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Hexokinase II with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img25308.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Immunohistochemical analysis of paraffin-embedded human pancreatic adenocarcinoma tissue labeling Hexokinase II with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Strong cytoplasmic staining in human pancreatic adenocarcinoma (PMID: 26137268) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunoprecipitation-img2315.jpg "Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Hexokinase II was immunoprecipitated from 0.35 mg HeLa (humanepithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: Anti-Hexokinase II antibody [EPR20839] ab209847 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Hexokinase II antibody [EPR20839] ab209847 in HeLa whole cell lysate.Exposure time: 30 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
All lanes: Immunoprecipitation - Anti-Hexokinase II antibody [EPR20839] (Anti-Hexokinase II antibody [EPR20839] ab209847)
Predicted band size: 102 kDa
Observed band size: 102 kDa
![Western blot - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--western-blot-img2850.jpg "Western blot - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Western blot - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Hexokinase II antibody [EPR20839] ab209847).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Hexokinase II antibody [EPR20839] ab209847 observed at 110 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-Hexokinase II antibody [EPR20839] ab209847 was shown to react with Hexokinase II in wild-type HEK-293 cells in western blot with loss of signal observed in HK2 knockout sample. Wild-type and HK2 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-Hexokinase II antibody [EPR20839] ab209847 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Hexokinase II antibody [EPR20839] (Anti-Hexokinase II antibody [EPR20839] ab209847) at 1/1000 dilution
Lane 1: Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: HK2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2: Western blot - Human HK2 (Hexokinase II) knockout HEK-293 cell line (Human HK2 (Hexokinase II) knockout HEK-293 cell line ab269485)
Lane 3: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 4: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 110 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img113235.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Hexokinase II with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in NIH/3T3 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img113131.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Clone EPR20839 (ab228819) has been successfully conjugated by Abcam. This image was generated using Anti-Hexokinase II antibody [EPR20839] (Alexa Fluor® 647). Please refer to Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] ab237314 for protocol details.
Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] ab237314 staining Hexokinase II in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 647 Anti-Hexokinase II antibody [EPR20839] ab237314 at 1/1000 dilution (shown in red) and Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Immunohistochemical analysis of paraffin-embedded human heart tissue labeling Hexokinase II with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granularly cytoplasmic staining in human heart (PMID: 4058069; PMID: 26722360) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--flow-cytometry-intracellular-img413915.jpg "Flow Cytometry (Intracellular) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
Flow Cytometry (Intracellular) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Hexokinase II with Anti-Hexokinase II antibody [EPR20839] ab209847 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Hexokinase II antibody [EPR20839] ab209847).
![Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunocytochemistry-immunofluorescence-img113131.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819), expandable thumbnail](https://content.abcam.com/products/images/hexokinase-ii-antibody-epr20839-bsa-and-azide-free-ab228819--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819)")
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