Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)

Rabbit Recombinant Monoclonal Hexokinase Type III/HK3 antibody. Suitable for Dot, IHC-P, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

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Images

Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Dot	IHC-P	WB	ICC/IF
Human	Expected	Tested	Tested	Not recommended
Mouse	Expected	Not recommended	Tested	Not recommended
Recombinant fragment - Human	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1/1000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Recombinant fragment - Human	Dilution info -	Notes -

Target data

See full target information HK3

Function

Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed:8717435). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed:8717435).

Alternative names

Hexokinase-3, Hexokinase type III, Hexokinase-C, HK III, HK3

Rabbit Recombinant Monoclonal Hexokinase Type III/HK3 antibody. Suitable for Dot, IHC-P, WB and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR29196-29
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for human ICC and Ms IHC-P.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Dot blot analysis of Hexokinase Type III/HK3 using ab321996 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane1: His-tagged human HK3 fragment

Lane2: His-tagged human HK2 fragment

Lane3: His-tagged human HK1 fragment

Lane4: His-tagged human HKDC1 fragment
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human HK1, HK2 and HKDC1.
All lanes: Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution
Lane 1: His-tagged human HK3 fragment at 5 ng
Lane 2: His-tagged human HK2 fragment at 5 ng
Lane 3: His-tagged human HK1 fragment at 5 ng
Lane 4: His-tagged human HKDC1 fragment at 5 ng
Secondary
All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: brain, skeletal muscle (PMID: 8812439).
The bands beneath the target band (99 kDa) are likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution
Lane 1: Human liver cancer tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Mouse brain tissue lysate at 20 µg
Lane 6: Mouse skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 99 kDa, 36 kDa
Exposure time: 3s
Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: HEK-293 (PMID: 21072205).
Low expression: cerebellum, skeletal muscle (PMID: 8812439).
The bands beneath the target band (99 kDa) are likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution
Lane 1: Human lung tissue lysate at 20 µg
Lane 2: Human cerebellum tissue lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Lane 4: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 5: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 6: Human colon tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 99 kDa, 36 kDa
Exposure time: 3s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining observed in immune cells within human lung tissue.

The section was incubated with ab321996 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human cerebrum.

The section was incubated with ab321996 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining in human spleen.

The section was incubated with ab321996 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining observed in immune cells within human lung cancer tissue.

The section was incubated with ab321996 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996)
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: No staining in human skeletal muscle.

The section was incubated with ab321996 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins