Anti-Hexokinase Type III/HK3 antibody [EPR29196-29]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining observed in immune cells within human lung tissue.
The section was incubated with ab321996 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining observed in immune cells within human lung cancer tissue.
The section was incubated with ab321996 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human skeletal muscle.
The section was incubated with ab321996 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human cerebrum.
The section was incubated with ab321996 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Hexokinase Type III/HK3 with ab321996 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human spleen.
The section was incubated with ab321996 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HEK-293 (PMID : 21072205).

Low expression : cerebellum, skeletal muscle (PMID : 8812439).

The bands beneath the target band (99 kDa) are likely to be degraded target fragments.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 5:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 6:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 99 kDa,36 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain, skeletal muscle (PMID : 8812439).

The bands beneath the target band (99 kDa) are likely to be degraded target fragments.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution

Lane 1:

Human liver cancer tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 99 kDa,36 kDa

false

Exposure time: 3s

• Dot

Supplier Data

Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (AB321996)

Dot blot analysis of Hexokinase Type III/HK3 using ab321996 at 1 : 1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human HK3 fragment
Lane2 : His-tagged human HK2 fragment
Lane3 : His-tagged human HK1 fragment
Lane4 : His-tagged human HKDC1 fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human HK1, HK2 and HKDC1.

All lanes:

Dot Blot - Anti-Hexokinase Type III/HK3 antibody [EPR29196-29] (ab321996) at 1/1000 dilution

Lane 1:

His-tagged human HK3 fragment at 5 ng

Lane 2:

His-tagged human HK2 fragment at 5 ng

Lane 3:

His-tagged human HK1 fragment at 5 ng

Lane 4:

His-tagged human HKDC1 fragment at 5 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s